Gene:
Gene Symbol:
Pput_0379
Gene Name:
DNA polymerase III subunit epsilon
Original Clone ID:
11088d4BCt1p1
Keyword:
None
Species:
Pseudomonas putida F1
Type:
cDNA
Vector Name:
pSGX3
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
PpCD00423785
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 639nts
Open reading frame: 47 to 685
AAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCTTGGGGTATGTACCCT
GCGTGAGCAGCGCTGGGTGGTGCTGGACCTGGAAACCAGCGGGCTCAACCCCAACCGTGA
TCAGGTACTGTCGATCGGGGCGGTGGCCATCGAAGACGGTGCCATCGACTTTGCCCAGCA
GTTCGAACGCACACTGCACCGGCCTTTGCAGAAGACCAACGCCAGCGTACTGATCCACGG
TTTGGGCCCCAGCGCCCTGGCTGCCGGTTGCGACCCGGCCGAGGCCCTGCTGGATTTGCT
CGATTTCATTGGCAACAGCCCGGTGCTGGCGTTTCACGCGCCATTCGACCAGCGCATGCT
GGCGCGTGCGCTGAAAGAGAGCCTGGGTTACCGCTTGCAGTCGCCATTCCTCGATATCGC
CGAGCTGGCGCCGATGCTCAACCCCGACACCGTGCTGCGCGAGGCCGGGCTGGATGACTG
GGTAGCGCGGTTTGGCTTGCAGGTTGAAGAACGCCATCATGCCAGTGCCGATGCCCAGGT
GACCGCAGAACTGGCGCTGATCCTGTTCAGCCAGGCCCGGCGCCAGCAGCTGGACAGCCC
GCTGCAGCTGGAGCAACGGTTGCGCGGGTGGCGACGGCGCAAGATGCAGAGTCATGGTGA
AGGGCATCATCACCATCACCACTGATCCGAATTCGAGCTCCGTCGAC
Protein sequence predicted based on nucleotide sequence
GVCTLREQRWVVLDLETSGLNPNRDQVLSIGAVAIEDGAIDFAQQFERTLHRPLQKTNAS VLIHGLGPSALAAGCDPAEALLDLLDFIGNSPVLAFHAPFDQRMLARALKESLGYRLQSP FLDIAELAPMLNPDTVLREAGLDDWVARFGLQVEERHHASADAQVTAELALILFSQARRQ QLDSPLQLEQRLRGWRRRKMQSHGEGHHHHHH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t90c,I30I;t148c,L50L;
c195t,G65G;a210c,A70A;
t211c,L71L;t241c,L81L;
c318g,A106A;c335g,T112S;
t339g,L113L;g342t,G114G;
c343t,t345c,H115Y;a357g,S119S;
a358c,t359c,g360a,M120P;a366c,L122L;
c369t,D123D;g370a,t372c,V124I;
a378g,E126E;a384g,A128A;
a399c,P133P;g405c,T135T;
a408g,V136V;g438a,V146V;
a441g,A147A;t498c,T166T;
t564g,L188L
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-11088d
Recommended expression in:
Not Applicable
Vector Name:
pSGX3
Synonyms:
pSGX-3, pSGX3_BC
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
Modified from pET26b from Novagen
Size (bp):
5339
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
initiation start (Met, Ser, Leu)
5072
5079
TOPO recognition site
TOPO
TOPO cloning site
5076
5085
bacterial origin
ori
pBR322-type bacterial origin of replication
1421
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4983
5003
repressor protein gene
LacI
LacI CDS
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
6xHis tag
5086
5103
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 terminator
5231
5307
