NFIB (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  NFIB
Gene Name:  nuclear factor I/B
Original Clone ID:
5563.1.44_GO.36496
Keyword:
full-length cds
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
Linker not verified. Low quality silent and/or missense mutations found in CDS.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
HsCD00424004
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1281nts
Open reading frame: 26 to 1306

AACTTTGTACAAAAAAGCAGGCTTCGAAAACCCGTACTTCCAGTCCACGTATTCTCCCAT
CTGTCTCACTCAGGATGAATTTCACCCATTCATCGAGGCACTTCTTCCACATGTCCGTGC
AATCGCCTATACTTGGTTCAACCTGCAGGCTCGAAAACGCAAGTACTTTAAAAAGCATGA
GAAGCGAATGTCAAAGGATGAAGAAAGAGCAGTCAAAGATGAGCTTCTCAGTGAAAAGCC
TGAAATCAAACAGAAGTGGGCATCCAGGCTCCTTGCCAAACTGCGCAAAGATATTCGCCA
GGAGTATCGAGAGGACTTTGTGCTCACCGTGACTGGCAAGAAGCACCCGTGCTGTGTCTT
ATCCAATCCCGACCAGAAGGGTAAGATTAGGAGAATCGACTGCCTGCGACAGGCAGACAA
AGTCTGGCGTCTGGATCTAGTCATGGTGATCCTGTTCAAAGGCATCCCCTTGGAAAGTAC
CGATGGAGAGCGGCTCATGAAATCCCCACATTGCACAAACCCAGCACTTTGTGTCCAGCC
ACATCATATCACAGTATCAGTTAAGGAGCTTGATTTGTTTTTGGCATACTACGTGCAGGA
GCAAGATTCTGGACAATCAGGAAGTCCAAGCCACAATGATCCTGCCAAGAATCCTCCAGG
TTACCTTGAGGATAGTTTTGTAAAATCTGGAGTCTTCAATGTATCAGAACTTGTAAGAGT
ATCCAGAACGCCCATAACCCAGGGAACTGGAGTCAACTTCCCAATTGGAGAAATCCCAAG
CCAACCATACTATCATGACATGAACTCGGGGGTCAATCTTCAGAGGTCTCTGTCTTCTCC
ACCAAGCAGCAAAAGACCCAAAACTATATCCATAGATGAAAATATGGAACCAAGTCCTAC
AGGAGACTTTTACCCCTCTCCAAGTTCACCAGCTGCTGGAAGTCGAACATGGCACGAAAG
AGATCAAGATATGTCTTCTCCGACTACTATGAAGAAGCCTGAAAAGCCATTGTTCAGCTC
TGCATCTCCACAGGATTCTTCCCCAAGACTGAGCACTTTCCCCCAGCACCACCATCCCGG
AATACCTGGAGTTGCACACAGTGTCATCTCAACTCGAACTCCACCTCCACCTTCACCGTT
GCCATTTCCAACACAAGCTATCCTTCCTCCAGCCCCATCGAGCTACTTTTCTCATCCAAC
AATCAGATATCCTCCCCACCTGAATCCTCAGGATACTCTGAAGAACTATGTACCTTCTTA
TGACCCATCCAGTCCACAAACCAGCCAGTCCTGGTACCTGGGCTAGTAGGACC

Protein sequence predicted based on nucleotide sequence

ENPYFQSTYSPICLTQDEFHPFIEALLPHVRAIAYTWFNLQARKRKYFKKHEKRMSKDEE
RAVKDELLSEKPEIKQKWASRLLAKLRKDIRQEYREDFVLTVTGKKHPCCVLSNPDQKGK
IRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLMKSPHCTNPALCVQPHHITVSVK
ELDLFLAYYVQEQDSGQSGSPSHNDPAKNPPGYLEDSFVKSGVFNVSELVRVSRTPITQG
TGVNFPIGEIPSQPYYHDMNSGVNLQRSLSSPPSSKRPKTISIDENMEPSPTGDFYPSPS
SPAAGSRTWHERDQDMSSPTTMKKPEKPLFSSASPQDSSPRLSTFPQHHHPGIPGVAHSV
ISTRTPPPPSPLPFPTQAILPPAPSSYFSHPTIRYPPHLNPQDTLKNYVPSYDPSSPQTS
QSWYLG*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t8c,L3P;t23c,M8T;t99c,I33I
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.36496
Recommended expression in:
Not Applicable