RASSF9 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  RASSF9
Gene Name:  Ras association (RalGDS/AF-6) domain family (N-terminal) member 9
Original Clone ID:
5563.1.57_GO.36994
Keyword:
full-length cds
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
Linker not verified. Low quality silent and/or missense mutations found in CDS.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
HsCD00424014
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1326nts
Open reading frame: 18 to 1343

ACAAAAAAGCAGGCTCCGAAAACCCGTACTTCCAGTCCGCTCCCTTTGGAAGAAACTCGC
TAAAGACTCGGCATAAAAACAGATCTCCAACTAAAGACATGGATTCAGAAGAGAAGGAAA
TTGTGGTTTGGGTTTGCCAAGAAGAGAAGCTTGTCTGTGGGCTGACTAAACGCACCACCT
CTGCTGATGTCATCCAGGCTTTGCTTGAGGAACATGAGGCTACGTTTGGAGAGAAACGAT
TTCTTCTGGGGAAGCCCAGTGATTACTGCATCATAGAGAAGTGGAGAGGCTCCGAAAGGG
TTCTTCCTCCACTAACTAGAATCCTGAAGCTTTGGAAAGCGTGGGGAGATGAGCAGCCCA
ATATGCAATTTGTTTTGGTTAAAGCAGATGCTTTTCTTCCAGTTCCTTTGTGGCGGACAG
CTGAAGCCAAATTAGTGCAAAACACAGAAAAATTGTGGGAGCTCAGCCCAGCAAACTACA
TGAAGACTTTACCACCAGATAAACAAAAAAGAATAGTCAGGAAAACTTTCCGGAAACTGG
CTAAAATTAAGCAGGACACAGTTTCTCATGATCGAGATAATATGGAGACATTAGTTCATC
TGATCATTTCCCAGGACCATACTATTCATCAGCAAGTCAAGAGAATGAAAGAGCTGGATC
TGGAAATTGAAAAGTGTGAAGCTAAGTTCCATCTTGATCGAGTAGAAAATGATGGAGAAA
ACTATGTTCAGGATGCATATTTAATGCCCAGTTTCAGTGAAGTTGAGCAAAATCTAGACT
TGCAGTATGAGGAAAACCAGACTCTGGAGGACCTGAGCGAAAGTGATGGAATTGAACAGC
TGGAAGAACGACTGAAATATTACCGAATACTCATTGATAAGCTCTCTGCTGAAATAGAAA
AAGAGGTAAAAAGTGTTTGCATTGATATAAATGAAGATGCGGAAGGGGAAGCTGCAAGTG
AACTGGAAAGCTCTAATTTAGAGAGTGTTAAGTGTGATTTGGAGAAAAGCATGAAAGCTG
GTTTGAAAATTCACTCTCATTTGAGTGGCATCCAGAAAGAGATTAAATACAGTGACTCAT
TGCTTCAGATGAAAGCAAAAGAATATGAACTCCTGGCCAAGGAATTCAATTCACTTCACA
TTAGCAACAAAGATGGGTGCCAGTTAAAGGAAAACAGAGCGAAGGAATCTGAGGTTCCCA
GTAGCAATGGGGAGATTCCTCCCTTTACTCAAAGAGTATTTAGCAATTACACAAATGACA
CAGACTCGGACACTGGTATCAGTTCTAACCACAGTCAGGACTCCGAAACAACAGTAGGAG
ATGTGGTGCTGTTGTCAACATAGTAGGACC

Protein sequence predicted based on nucleotide sequence

ENPYFQSAPFGRNSLKTRHKNRSPTKDMDSEEKEIVVWVCQEEKLVCGLTKRTTSADVIQ
ALLEEHEATFGEKRFLLGKPSDYCIIEKWRGSERVLPPLTRILKLWKAWGDEQPNMQFVL
VKADAFLPVPLWRTAEAKLVQNTEKLWELSPANYMKTLPPDKQKRIVRKTFRKLAKIKQD
TVSHDRDNMETLVHLIISQDHTIHQQVKRMKELDLEIEKCEAKFHLDRVENDGENYVQDA
YLMPSFSEVEQNLDLQYEENQTLEDLSESDGIEQLEERLKYYRILIDKLSAEIEKEVKSV
CIDINEDAEGEAASELESSNLESVKCDLEKSMKAGLKIHSHLSGIQKEIKYSDSLLQMKA
KEYELLAKEFNSLHISNKDGCQLKENRAKESEVPSSNGEIPPFTQRVFSNYTNDTDSDTG
ISSNHSQDSETTVGDVVLLST*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t8c,L3P;t41c,L14S
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.36994
Recommended expression in:
Not Applicable