Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
5541.1.74_GO.34675
Keyword:
full-length cds (mutant)
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
ScCD00424035
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 2115nts
Open reading frame: 1 to 2115

GAAAACCTGTACTTCCAGTCCGTGGATAAAAATAGGACGTTGCATGAATTATCTTCCAAA
AATTTCAGCAGAACGCCGAATGGACTGATCTTTACCAATGATTTAAAGACAGTATATTCC
ATTTTTCTAATTTGTTTGGATTTGAAAGAGAAAAAACATAGTAGTGACACTAAGTCTTTC
TTATTAACTGCATTCACTAAACATTTCCACTTCACTTTTACATATCAGGAAGCTATTAAA
GCAATGGGACAATTGGAACTAAAAGTGGATATGAACACAACTTGTATTAACGTTTCATAT
AATATTAAACCTAGTTTGGCGCGCCACCTTCTTACATTATTTATGTCTTCTAAGCTACTA
CATACACCGCAGGACAGAACTCGTGGCGAGCCAAAAGAGAAAGTTCTTTTCCAACCAACA
CCTAAGGGAGTTGCTGTTTTACAAAAGTATGTTAGAGATATTGGGTTGAAAACAATGCCT
GATATTCTTTTGTCATCGTTTAACTCCATGAAACTTTTCACTTTTGAGAGAAGCTCTGTC
ACGGATTCAATCATCCATAGTGATTATTTGATCCATATTCTTTTCATTAAAATGATGGGC
GCTAAGCCAAATGTTTGGTCTCCAACAAACGCAGATGACCCATTACCTTGCCTATCTTCG
TTGTTGGAATACACTAATAATGATGATACATTTACTTTTGAGAAATCTAAGCCAGAACAA
GGATGGCAAGCTCAGATTGGAAACATCGATATAAATGACTTAGAAAGGGTATCGCCATTA
GCTCATCGATTCTTTACAAATCCAGATTCTGAGTCGCACACGCAATACTACGTTTCGAAT
GCAGGTATTAGGTTGTTTGAAAATAAGACGTTTGGCACTTCCAAAAAAATCGTGATTAAG
TACACCTTTACAACAAAGGCTATATGGCAGTGGATAATGGACTGTACTGATATTATGCAT
GTTAAAGAAGCGGTCTCGTTGGCAGCGTTATTTTTAAAAACGGGGTTGATTGTACCAGTT
CTTTTACAACCATCACGCACTGACAAGAAAAAGTTTCAAATTAGCAGGTCCTCTTTTTTT
ACCTTGTCCAAGCGTGGTTGGGATTTGGTTTCTTGGACAGGTTGTAAGAGCAATAATATT
AGAGCACCAAATGGTAGCACCATAGACCTAGATTTCACATTAAGGGGTCATATGACTGTT
CGTGATGAAAAAAAGACATTGGATGATAGTGAAGGTTTTTCTCAAGACATGCTTATCTCT
TCGAGTAATTTAAATAAACTGGACTATGTGCTAACTGATCCAGGTATGAGATATTTATTC
AGGCGCCATTTAGAAAAGGAACTTTGTGTAGAAAATTTGGATGTCTTCATTGAGATTAAG
AGGTTTTTGAAGAAAATGACAATTCTGAAAAAATTGATAGATTCTAAGCATTGTGATAAA
AAATCTAATACCAGTACTTCAAAAAACAATATCGTAAAAACGATTGATTCAGCTTTAATG
AAACAGGCCAATGAGTGCCTTGAAATGGCATATCACATTTATTCGTCCTATATCATGATA
GGATCACCATATCAATTAAATATCCATCATAACTTGCGTCAGAATATTTCTGACATCAAG
TTGCACCCTCATTCTCCATTATCGGAACACTTTCCTACTAATCTATACGATCCATCACCT
GCATCCGCGGAATCAGCAGCTTCATCAATTAGCTCAACGGAGGCTGACACGTTAGGTGAA
CCACCTGAAGTGAGTCTAAAACCTTCAAAAAATCTTTCAAATGAAAACTGTTCATTTAAG
AAGCAAGGATTCAAACATCAGTTAAAGGAATATAAACCTGCCCCGTTAACTCTGGCAGAA
ACGCATTCGCCTAATGCATCAGTAGAAAATTCACATACAATTGTTCGTTATGGCATGGAC
AACACCCAAAATGATACCAAATCGGTTGATTCATTTCCAGCAACTTTGAAGGTCCTCAGG
AAATTATATCCCCTCTTCGAGATCGTTAGCAATGAAATGTATAGACTGATGAATAACGAT
TCCTTTCAAAAATTTACCCAGTCAGACGTATACAAAGATGCTAGCGCTTTAATAGAAATC
CAAGAAAAGTGCTAATAG

Protein sequence predicted based on nucleotide sequence

ENLYFQSVDKNRTLHELSSKNFSRTPNGLIFTNDLKTVYSIFLICLDLKEKKHSSDTKSF
LLTAFTKHFHFTFTYQEAIKAMGQLELKVDMNTTCINVSYNIKPSLARHLLTLFMSSKLL
HTPQDRTRGEPKEKVLFQPTPKGVAVLQKYVRDIGLKTMPDILLSSFNSMKLFTFERSSV
TDSIIHSDYLIHILFIKMMGAKPNVWSPTNADDPLPCLSSLLEYTNNDDTFTFEKSKPEQ
GWQAQIGNIDINDLERVSPLAHRFFTNPDSESHTQYYVSNAGIRLFENKTFGTSKKIVIK
YTFTTKAIWQWIMDCTDIMHVKEAVSLAALFLKTGLIVPVLLQPSRTDKKKFQISRSSFF
TLSKRGWDLVSWTGCKSNNIRAPNGSTIDLDFTLRGHMTVRDEKKTLDDSEGFSQDMLIS
SSNLNKLDYVLTDPGMRYLFRRHLEKELCVENLDVFIEIKRFLKKMTILKKLIDSKHCDK
KSNTSTSKNNIVKTIDSALMKQANECLEMAYHIYSSYIMIGSPYQLNIHHNLRQNISDIK
LHPHSPLSEHFPTNLYDPSPASAESAASSISSTEADTLGEPPEVSLKPSKNLSNENCSFK
KQGFKHQLKEYKPAPLTLAETHSPNASVENSHTIVRYGMDNTQNDTKSVDSFPATLKVLR
KLYPLFEIVSNEMYRLMNNDSFQKFTQSDVYKDASALIEIQEKC*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t1387n;a1619t,K540M
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.34675
Recommended expression in:
Not Applicable