Gmet_1302 (G. metallireducens GS-15 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Gmet_1302
Gene Name:  PhoH-related ATPase
Original Clone ID:
62140
Keyword:
full-length cds
Species:
Geobacter metallireducens GS-15
Type:
cDNA
Vector Name:
pSpeedET
Format: 
FUSION
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
Linker not verified. Low quality silent and/or missense mutations found in CDS.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
GmCD00424130
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1317nts
Open reading frame: 51 to 1367

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGAAAAACT
TCGTACTCGATACCAACGTCCTCCTCTACGATCCCCAGGCCATTTTCAAGTTTGAGGAAA
ACAACATCATTATCCCCATTACGGTCATCGAAGAGGTGGACCGCTTCAAAAAGGACATGA
ACGAGACGGGACGCAACGCACGCCAGGTCTCCCGTTATCTGGACGACATGCGCAAGATTG
GGTCCCTCTCCTCGGGCATTACCCTCGAGACCGGCGGGATGCTCCGGGTGGAGATGTACG
AGGAGAAGATCATGAAGCGGCTTCCTCCCGAGTTGCGCGAGGAGCGGGGGGACAACCGGA
TACTGGCGGTGGCCGTCAAGATTCTGGAAGAAAACGGTGGCGCGCCCGTCGTGTTCGTCA
CCAAGGATACGAACTTGCGGATAAAGGCCGACGCCATCGGCCTCAAGGCCGAGGATTACG
AATCGGATAAGGTCGACATCGAAGAGCTCTACCCCGGTTTCACCGAGATCGACGTGGCGG
CCGATACCGTTGACCGGTTCCATGGCCAGGGGTGGGTGGAGTTGCCCGGACAGACCTTCT
TCCCGAACCAGTGCGTCACCCTGCGGGATGAAGCGAACCCTTCCCATACGGCCCTCGGCA
AGTACCGGGTGGGGGATGGGAAAATCACTCCCCTCATCAAGACCGGCAAGGAGGGGGTCT
GGAGCATTTTCCCCCGCAACCGCGAGCAGGCCTTTGCCTTCGATCTTCTACTCGACGACT
CCATCAAGCTCGTGACCCTCGTGGGGAAGGCCGGTACAGGCAAGACGCTGCTGGCCATTG
CTGCGGGCCTCCACAAGACCGCCGAGGAGAACGTCTTCAACCGCCTTCTCGTCTCCCGGC
CCGTCTTTCCCATGGGGCGCGACCTGGGGTTCCTTCCCGGAGATATCGAGGAGAAGCTCG
CCCCCTGGATGCAGCCCATCTTCGACAACGTGGAACTTCTCCTGTCGGGGCATGAGGCCG
AGAAGCGCCACAGCAAGGGGTATAAGGAGCTCATGGCCATGGGGATCATGGAAATTGAGC
CCCTCACCTACATCCGGGGTCGCTCCATACCGAACCAGTTCATGATCGTCGACGAGGCCC
AGAACCTCACGCCTCACGAGATCAAGACCATCATTACCCGGGCTGGGGAGGGGACCAAGA
TCGTCCTCACCGGCGACCCCTACCAGATTGACAACCCCTATGTGGATGCCTCGTCCAACG
GCCTTACCTACGTGGTGGAGCGCTTCAAGGAGCAGGCAATCGCCGGCCATGTCACCATGA
CCAAGGGCGAGCGCTCCGACCTGGCGGAACTGGCGGCGAATCTGCTGTA

Protein sequence predicted based on nucleotide sequence

MKNFVLDTNVLLYDPQAIFKFEENNIIIPITVIEEVDRFKKDMNETGRNARQVSRYLDDM
RKIGSLSSGITLETGGMLRVEMYEEKIMKRLPPELREERGDNRILAVAVKILEENGGAPV
VFVTKDTNLRIKADAIGLKAEDYESDKVDIEELYPGFTEIDVAADTVDRFHGQGWVELPG
QTFFPNQCVTLRDEANPSHTALGKYRVGDGKITPLIKTGKEGVWSIFPRNREQAFAFDLL
LDDSIKLVTLVGKAGTGKTLLAIAAGLHKTAEENVFNRLLVSRPVFPMGRDLGFLPGDIE
EKLAPWMQPIFDNVELLLSGHEAEKRHSKGYKELMAMGIMEIEPLTYIRGRSIPNQFMIV
DEAQNLTPHEIKTIITRAGEGTKIVLTGDPYQIDNPYVDASSNGLTYVVERFKEQAIAGH
VTMTKGERSDLAELAANLL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 401476
Recommended expression in:
Not Applicable