amgS (P. aeruginosa PAO1 ) in pNYCOMPS-LIC-FH10T+ (N term)

Explanation of Terms

Gene:
Gene Symbol:  amgS
Gene Name:  protein AmgS
Original Clone ID:
GE.544.1.57_GO.11886
Keyword:
None
Species:
Pseudomonas aeruginosa PAO1
Type:
cDNA
Vector Name:
pNYCOMPS-LIC-FH10T+ (N term)
Format: 
CLOSED
Source:
New York Consortium on Membrane Protein Structure
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York Consortium on Membrane Protein Structure
PaCD00424349
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1320nts
Open reading frame: 51 to 1370

ACCATCACCATCACCACCATCACGAAAACCTGTATTTTCAATCCTACGTAATGAAAACGC
CGCTCTGGTTCCCGCAAAGCTTCTTCGCCCGCACCCTCTGGCTGGTGCTGATCGTCGTGC
TGTTCTCCAAGGCGCTGACCCTGGTCTACCTGCTGATGAACGAGGACGTGATCGTCGACC
GCCAGTACAGCCACGGCGCGGCGCTGACCATCCGCGCGTTCTGGGCTGCCGACGAGGAGT
CGCGCGCGGCCATCGCCAAGGCTTCCGGCCTGCGCTGGGTGCCGAGCAGCGCCGACCAGC
CCGGCGAGCAGCACTGGCCGTACACAGAAATCTTCCAGCGGCAGATGCAGATGGAGCTGG
GGCCGGACACCGAGACCCGCCTGCGTATCCACCAGCCGTCGCAGCTCTGGGTGCGCGCAC
CGAGCCTCGGCGAAGGCTGGCTGGCGGTGCCGCTGTATCCGCATCCCTTGCGTGGCCAAC
GGATCTGGAGCGTGCTCGGCTGGTTCCTCGGCATCGGCCTGCTCTCCACCGCCGCCGCCT
GGATCTTCGTCCGCCAGCTCAGCCAGCCGCTCAAGCGCCTGGTGGTCGCCGCCCGCCAGT
TCGGCCAGGGGCGCAGCGTGCGCCTGCCGCTGGGACCGGAGACGCCGAGTGAAATGGCCG
AGGTGTATCGCGCCTTCAACCAGATGGCCGAGGACATCGAGCAGGGCGGGCGCGAACGCG
AATTGATGTTGGCCGGTGTCTCCCACGACCTGCGCACCCCGCTGACGCGCCTGCGCCTGT
CGCTGGAGTTGCTGCCGGAGAGCGAACGGGAGATGGTCGAGGACATGATCCGCGACATCG
AGGACATGGACGCGATCCTCGACCAGTTCCTCGCCTTCATCCGCGACGGCCGCGACGAGC
CGGTGGAGGAGGGCGACCTGACCGACCTGGTGCGCGAGGTCGTGGCGCCGTTCAACCAGA
CTCGCGAGCAGGTGCGCATGGCCTTGCAACCGGTACCGGCGATGCCGTTGCGGCGTGTGT
CGATGAAGCGTCTGCTGGGCAACCTGATCGACAATGCCCTGAACCACGGCGGCGGTTCGG
TGGAGGTGGCCAGCTACGTGGCCGGCGAAAGCGCGGCGCCCTACGTGGTGCTGAGCGTGC
TCGATCGCGGCCAGGGCATCGATCCCGCGGAAGTGGACAGCATCTTCAATCCCTTCATCC
GTGGCGACAAGGCGCGTGGCGGCAAGGGCACCGGCCTCGGCCTGGCGATCGTCAAGCGCA
TCGCCGCCCAGCACGGCGGCAGCGTCGAACTGCGCAACCGCGATGGCGGCGGCCTCGAAG
CGCGGGTCTGCCTGCCGCTGGGGTTGCTGCTGCCGCGCGGCGCGGCCTAG

Protein sequence predicted based on nucleotide sequence

MKTPLWFPQSFFARTLWLVLIVVLFSKALTLVYLLMNEDVIVDRQYSHGAALTIRAFWAA
DEESRAAIAKASGLRWVPSSADQPGEQHWPYTEIFQRQMQMELGPDTETRLRIHQPSQLW
VRAPSLGEGWLAVPLYPHPLRGQRIWSVLGWFLGIGLLSTAAAWIFVRQLSQPLKRLVVA
ARQFGQGRSVRLPLGPETPSEMAEVYRAFNQMAEDIEQGGRERELMLAGVSHDLRTPLTR
LRLSLELLPESEREMVEDMIRDIEDMDAILDQFLAFIRDGRDEPVEEGDLTDLVREVVAP
FNQTREQVRMALQPVPAMPLRRVSMKRLLGNLIDNALNHGGGSVEVASYVAGESAAPYVV
LSVLDRGQGIDPAEVDSIFNPFIRGDKARGGKGTGLGLAIVKRIAAQHGGSVELRNRDGG
GLEARVCLPLGLLLPRGAA*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
Find experimental protocols in GO.11886
Recommended expression in:
Not Applicable