VPA0149 (V. parahaemolyticus RIMD 2210633 )
In pNYCOMPS-LIC-FH10T+ (N term)

Explanation of Terms

Gene:
Gene Symbol:  VPA0149
Gene Name:  two-component system sensor kinase
Original Clone ID:
GE.544.1.46_GO.12512
Keyword:
None
Species:
Vibrio parahaemolyticus RIMD 2210633
Type:
cDNA
Vector Name:
pNYCOMPS-LIC-FH10T+ (N term)
Format: 
CLOSED
Source:
New York Consortium on Membrane Protein Structure
Description:
None
Comments:
High number of silent and/or missense mutations in CDS.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York Consortium on Membrane Protein Structure
VpCD00424356
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1377nts
Open reading frame: 51 to 1427

ACCATCACCATCACCACCATCACGAAAACCTGTATTTTCAATCCTACGTAATGATGAAGC
TGGTGTGTCCATCCTACATAGCAAAACGCAAAGACAGCCTTGCGTTTAAGCTTTTCAGTT
ACATGACCGCGATAGTCATCAGCATTTTGCTGGTGCAAGCGTTAGCGGAACAAGCCTTGG
TGAAAGCTATGTTGAAAGTTCCAAATAGCGTCAAAATGGAAATGTTGGATCTTGCTCACC
AAGCCAACGTGCTGATTGAACATGGCGACATGGATGAGTTGGCTGATTGGGCGAACGCGC
AGCAGTTCTATTTGTTTGTACTGAACGAGCAACTTCAACCAGTGACGCATCGTCTCATGC
ATCCACATTTTGAATTTAAGCTCCAATTCAAACGCAGCATTGATGATCAATTGGAAGACA
AAGTGAATCGACCAATCATCAGCATTCCATTGGTTCAGCAGCACAACTTAGTCATTCAAC
TGCCGCATCGCTTACATCCTGCGAATGACTTCATACTGTACTTTTCCATTATCAAAGTGG
TAATTGCCGCCGTGATTTTGGCTTTGTTCTCGCTTTTAATGGCACGCAACTTACAGCAAC
CGTTGGACAGACTCAGAGAGGCGAGCAAGAAGCTTTCTAACGGCGATTTTAATGTCAGTG
TTGTGCAGGAGCTAAAGTCCAACACGCGAGAATTTAATGAACTCGCGCAAGATTTTGACC
ACATGACGAAAGAGATTCACTCGCTAGCGGAGAAGCAACGTCTTTTGATTCGCGATGTGT
CCCATGAACTAAGAACGCCTCTTGCTAGACAGAACTTGGTTTTGCACCTATTGCGTAAAA
GAGTTGGTGAAAGCGAACAAGAACTAGTGACGCGGTTGGAAGACGAAGCCAAAGAGCTGA
ATCATCTAATCAGTGACATTTTGGAATTTAGTCGCCTTGAGAATGCTCGCTACGACAAAG
AGACCAAGCGTAGTGATCTTGAAGTGCTACTAGCCCCACAAGTTGAGCGTTGCAAAATCG
ATTTGAGGGAATCACAGAGCATTGTATTTATTTCTCGTGTGACCGAGCCGAGAGCTTACT
GTGAAGATACTCTCGTGGTGCGTTGTCTGAATAATCTAGTTGGCAACGCTATCAAATACG
CGGGTGAAGATGCTCGGATCGAAGTGACTCTGACTAATCAAGAAATCAATGGGCAGGCTT
ATCTGGTTATGTCTGTCTCTGACGATGGGCCTGGCATCCCAGAGAACAAGTTATCCGATA
TCTTTAACCCGTTTACCCGCTTGGATGCCTCGCGAGACAAAAAGCTTGGTGGATACGGCC
TTGGACTGGCGATCGTTAAGGAATCGATGCGAATGATGGGTGGTTCCGTCAATGCAGAGA
ATCGTTCTCAAGGTGGTTTAACCGTGAGTTTGGTGTTCAGACAATAA

Protein sequence predicted based on nucleotide sequence

MMKLVCPSYIAKRKDSLAFKLFSYMTAIVISILLVQALAEQALVKAMLKVPNSVKMEMLD
LAHQANVLIEHGDMDELADWANAQQFYLFVLNEQLQPVTHRLMHPHFEFKLQFKRSIDDQ
LEDKVNRPIISIPLVQQHNLVIQLPHRLHPANDFILYFSIIKVVIAAVILALFSLLMARN
LQQPLDRLREASKKLSNGDFNVSVVQELKSNTREFNELAQDFDHMTKEIHSLAEKQRLLI
RDVSHELRTPLARQNLVLHLLRKRVGESEQELVTRLEDEAKELNHLISDILEFSRLENAR
YDKETKRSDLEVLLAPQVERCKIDLRESQSIVFISRVTEPRAYCEDTLVVRCLNNLVGNA
IKYAGEDARIEVTLTNQEINGQAYLVMSVSDDGPGIPENKLSDIFNPFTRLDASRDKKLG
GYGLGLAIVKESMRMMGGSVNAENRSQGGLTVSLVFRQ*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c138t,A46A;g282a,Q94Q;
g492a,V164V;t534a,A178A;
t633c,N211N;t780a,L260L;
a783g,L261L;c790a,R264R;
t814c,L272L;c826t,a828g,L276L;
c924t,S308S;a1005t,S335S
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinExpressed: Tested_Not_Found
ProteinPurified: Tested_Not_Purified
Find experimental protocols in GO.12512
Recommended expression in:
Not Applicable