AF1035 (A. fulgidus DSM 4304 ) in pNYCOMPS-LIC-FH10T+ (N term)

Explanation of Terms

Gene:
Gene Symbol:  AF1035
Gene Name:  chemotaxis histidine kinase
Original Clone ID:
GE.540.1.90_GO.13334
Keyword:
None
Species:
Archaeoglobus fulgidus DSM 4304
Type:
cDNA
Vector Name:
pNYCOMPS-LIC-FH10T+ (N term)
Format: 
CLOSED
Source:
New York Consortium on Membrane Protein Structure
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York Consortium on Membrane Protein Structure
AfCD00424412
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1290nts
Open reading frame: 49 to 1338

CATCACCATCACCACCATCACGAAAACCTGTATTTTCAATCCTACGTAATGCGGTTGCCG
GCAAAACTTAAAAAGAATGCTTTTGGATGGGAAATTGCACCTTTGAAATTATTGAAGATA
ATTACACTTTTGAAGATTAGGTTTATTTGCGCACTTTCCATTATTCTTCAAATGTTCAGA
GATTATGGACTTTTCAAAGCCCTTGCGGAAATGAGCCCTCATCCGATAATTTTACTTGAT
GGAACTGTAATTCGCTACGCAAATCCAAAGGCGTTGCAGCTTATTGGTGAAAACTGTCTG
CAAAGAGATATTCTCGATTTTCTGGATGAAAACAGCGGTAGATGTTTAAGGCAGGCACTT
CGGAAAGATCGCGGGACTTTTGAGGTGAGGTTCAGGGGCAAGGCAGGTGCATTGAAGGCT
GATTTGTTCAAACTTGACTCTAAAGCCGTGCTCACCTTAAGGTTTGACGTTGAGTCAGTT
TACAGGAAAATTTTCGAGACTGCACCTTCCCCCATAGCGGTTGTTGACTTAAATGGTGTT
GTGAGGGATGCGAACTTCTCCATGAAAGGATTTGCGGGATTCAACATCATCGGAAAAAAG
CTCAGTGAGCTTTTCTCTCAGGAAAGAGCGGAAAGGATAATGAGTTATGTTAATTTGGCT
TTGATGGAGAATACCGTGGTTGAGTTTGACGAAGAACTGGGAGACAGAAGGCTTGTAAGC
TACTGCATTCCTGCTGAGTTGGAGGGCGAAAGGCTTTGCTTTATCATTTCCCACGATGTA
ACCGAGTTTTACAGACTGAACAGGCTGCTTGAAAGGATTGTGGAGGCAAGCGAAGCGATG
ATTAAACTCAGAAGCAAGAGCAAGCTCATAGAAAGTGTTGAAAGCATACTCTCAGATTAC
TCTGCAAGGGTCCTCGACAGACCTGAGGGTGTTGCATTTAAAATTGAGTTCGATAGTATT
AGTTACGGCTACCTCTGCGTTAAAAATGCAGATTACGCTGAGAGACGACTACTCAAAACT
CTTGCGAAAAACCTGGCGTTCGCTCTAAAAGCGGTTGAGGATGAGCTGAGGAGGGAGGAG
TTCTTAAAGAGGCTGGTTGAAAACACCAAGGTAATCGCCTATCTCGTTGACAGAATCAGG
AACCCGCTAACGGCAATCAGGGCTTTTGCCGAAGTAATGATATCCGACGATAAAGTGAGG
GAGAGGATAACTCAGCAGGTGGACAGAATCGTGGGCATCATCAAGAACCTTGATTCGTCG
TGGGCGAAGTCCGAGGAAATTGTTGGGAAGGTTGACGTTGCGGACCTTCTCTATTCCGAT
AGCGGATTTCAAAACTGA

Protein sequence predicted based on nucleotide sequence

MRLPAKLKKNAFGWEIAPLKLLKIITLLKIRFICALSIILQMFRDYGLFKALAEMSPHPI
ILLDGTVIRYANPKALQLIGENCLQRDILDFLDENSGRCLRQALRKDRGTFEVRFRGKAG
ALKADLFKLDSKAVLTLRFDVESVYRKIFETAPSPIAVVDLNGVVRDANFSMKGFAGFNI
IGKKLSELFSQERAERIMSYVNLALMENTVVEFDEELGDRRLVSYCIPAELEGERLCFII
SHDVTEFYRLNRLLERIVEASEAMIKLRSKSKLIESVESILSDYSARVLDRPEGVAFKIE
FDSISYGYLCVKNADYAERRLLKTLAKNLAFALKAVEDELRREEFLKRLVENTKVIAYLV
DRIRNPLTAIRAFAEVMISDDKVRERITQQVDRIVGIIKNLDSSWAKSEEIVGKVDVADL
LYSDSGFQN*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinExpressed: Tested_Not_Found
ProteinPurified: Tested_Not_Purified
Find experimental protocols in GO.13334
Recommended expression in:
Not Applicable