RNF8 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  RNF8
Gene Name:  ring finger protein 8, E3 ubiquitin protein ligase
Original Clone ID:
FLH165572.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00042754
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1455nts
Open reading frame: 1 to 1455

ATGGGGGAGCCCGGCTTCTTCGTCACAGGAGACCGCGCCGGTGGCCGGAGCTGGTGCCTG
CGGCGGGTGGGGATGAGCGCCGGGTGGCTGCTGCTGGAAGATGGGTGCGAGGTGACTGTA
GGACGAGGATTTGGTGTCACATACCAACTGGTATCAAAAATCTGCCCCCTGATGATTTCT
CGAAACCACTGTGTTTTGAAGCAGAATCCTGAGGGCCAATGGACAATTATGGACAACAAG
AGTCTAAATGGTGTTTGGCTGAACAGAGCGCGTCTGGAACCTTTAAGGGTCTATTCCATT
CATCAGGGAGACTACATCCAACTTGGAGTGCCTCTGGAAAATAAGGAGAATGCGGAGTAT
GAATATGAAGTTACTGAAGAAGACTGGGAGACAATATATCCTTGTCTTTCCCCAAAGAAT
GACCAAATGATAGAAAAAAATAAGGAATTGAGAACTAAAAGGAAATTCAGTTTGGATGAA
TTAGCAGGTCCTGGAGCTGAAGGCCCCTCAAATTTGAAATCCAAAATAAATAAAGTGTCT
TGTGAATCTGGTCAGCCAGTGAAATCACAGGGGAAAGGTGAAGTGGCCAGTACACCCTCT
GACAATTTGGATCCTAAGTTGACTGCCCTTGAGCCAAGTAAGACCACAGGGGCTCCCATT
TACCCTGGCTTCCCCAAAGTCACAGAGGTTCATCATGAGCAGAAAGCCTCAAACTCTTCA
GCATCTCAGAGAAGCTTACAGATGTTTAAGGTGACCATGTCCAGGATTCTGAGGCTCAAA
ATACAGATGCAGGAAAAACATGAAGCCGTTATGAATGTGAAAAAGCAGACCCAAAAGGGG
AACTCAAAGAAAGTTGTGCAAATGGAGCAGGAACTTCAGGACTTACAGTCCCAGCTGTGT
GCAGAGCAGGCTCAGCAGCAGGCAAGAGTGGAGCAACTAGAGAAGACTTTCCAGGAAGAG
GAACAGCATCTTCAGGGTTTGGAGATAGCCCAAGGAGAAAAGGACCTGAAGCAACAGCTG
GCCCAGGCTCTGCAGGAGCATTGGGCTCTAATGGAAGAGCTAAATCGCAGCAAGAAGGAC
TTTGAAGCAATCATTCAAGCCAAGAACAAAGAATTAGAGCAGACCAAGGAAGAGAAGGAG
AAGATGCAAGCACAGAAGGAAGAAGTTCTTAGCCACATGAATGATGTGCTAGAGAATGAG
CTCCAATGTATTATTTGTTCAGAATACTTCATTGAGGCTGTCACCTTGAACTGTGCCCAC
AGTTTCTGCTCCTACTGTATCAATGAATGGATGAAGCGGAAGATAGAATGCCCCATTTGT
CGGAAGGACATTAAGTCCAAAACATACTCTTTGGTTCTGGACAATTGCATTAATAAGATG
GTAAATAATCTGAGCTCAGAAGTGAAAGAACGACGAATTGTTCTCATTAGGGAACGAAAA
GCAAAGAGATTGTTC

Protein sequence predicted based on nucleotide sequence

MGEPGFFVTGDRAGGRSWCLRRVGMSAGWLLLEDGCEVTVGRGFGVTYQLVSKICPLMIS
RNHCVLKQNPEGQWTIMDNKSLNGVWLNRARLEPLRVYSIHQGDYIQLGVPLENKENAEY
EYEVTEEDWETIYPCLSPKNDQMIEKNKELRTKRKFSLDELAGPGAEGPSNLKSKINKVS
CESGQPVKSQGKGEVASTPSDNLDPKLTALEPSKTTGAPIYPGFPKVTEVHHEQKASNSS
ASQRSLQMFKVTMSRILRLKIQMQEKHEAVMNVKKQTQKGNSKKVVQMEQELQDLQSQLC
AEQAQQQARVEQLEKTFQEEEQHLQGLEIAQGEKDLKQQLAQALQEHWALMEELNRSKKD
FEAIIQAKNKELEQTKEEKEKMQAQKEEVLSHMNDVLENELQCIICSEYFIEAVTLNCAH
SFCSYCINEWMKRKIECPICRKDIKSKTYSLVLDNCINKMVNNLSSEVKERRIVLIRERK
AKRLF

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 132
End on reference sequence 1589
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable