SE0459 (S. epidermidis )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  SE0459
Gene Name:  cobalamin synthesis related protein
Original Clone ID:
38333
Keyword:
full-length cds
Species:
Staphylococcus epidermidis
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
SeCD00429748
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 927nts
Open reading frame: 51 to 977

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGAAAAATA
ATAAAAATGAAAAGTTGAAAATCACCATCGTTAGCGGTTTCTTAGGCAGTGGTAAAACTA
CGTTCCTGACACATTATATTAATGAATTATTAAAAATTGATGAGAAAATAACAGTAATTA
TGAACGAATTTGGATCATTTGATGTAGATGGTAACCATTTGAGTGAATTAGTCAAAGTGT
ATCCATTGTTGAACGGTTGTGTTTGTTGTGATATGAAATCAGATTTAGTTCAACAAATTG
AGATGCTCAGTCACAACCATGAAACGGACCATATTATAATAGAAGCAACTGGTATCGCGC
ATCCTATTGAATTGATTATGGCGTGTCAAGATCCTCGCATAGTTCAGTTTGTTGAACGTC
CCCACGTTGTTAGTATTGTTGATGCACTTCGCTTTTTACAAAGACAACAGTATACTGACT
CCACAAAGCGTCTCATGGAAGAACAACTCGAGGTAAGCGATGTGCTCGTCGTTAATAAAT
TAGATTTAATATCTGTTGAAGACAGAGATGCTTTAAAGAACCAACTTAACGAAGTAAACA
AATATAGTTCAAAAATATATACTACTTATGGACAAGTTAATATCGAGGAGTTGTCACAAT
TTCGTGATCAATTAGTAATGACACACACACATTCTCATCACCATGGTATAAATAGTATGC
AATACACATTTACAACAGCTATTGACCGACAATTATTTTACCAATTTATTTTGCGTATGC
CTGATAATGTTTTAAGATTAAAGGGATATGTACAATTTAAAGATGAACCCAATGTGACAT
ATGAGTTTCAATATTCATTTGGTATGCCGGATTATGAAGTTATTAATGGGGATTATCCGT
TAACAATAGTATTGATTGGTGAACAACTTGATGTTAACAGACTAAAAAATAAACTCGATG
TTTTGCAATTCTCATAACGCGACTTAATTAATTTGGCGGATGAGAGAAGATTTT

Protein sequence predicted based on nucleotide sequence

MKNNKNEKLKITIVSGFLGSGKTTFLTHYINELLKIDEKITVIMNEFGSFDVDGNHLSEL
VKVYPLLNGCVCCDMKSDLVQQIEMLSHNHETDHIIIEATGIAHPIELIMACQDPRIVQF
VERPHVVSIVDALRFLQRQQYTDSTKRLMEEQLEVSDVLVVNKLDLISVEDRDALKNQLN
EVNKYSSKIYTTYGQVNIEELSQFRDQLVMTHTHSHHHGINSMQYTFTTAIDRQLFYQFI
LRMPDNVLRLKGYVQFKDEPNVTYEFQYSFGMPDYEVINGDYPLTIVLIGEQLDVNRLKN
KLDVLQFS*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a36c,T12T
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 382956
Recommended expression in:
Not Applicable