53718365 (B. pseudomallei )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
9282h2BCt12p1
Keyword:
full-length cds
Species:
Burkholderia pseudomallei
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
BpCD00429762
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1122nts
Open reading frame: 42 to 1163

TATTTTGTTTACTTTAAGAAGGAGATATACATATGTCCCTTAAACCGTTTAGCGAGCCGA
TTATCCGTTGCGCGAAGGCCCGCGCCTATCGGATACCTACCGATAGACCCGAGGCTGACG
GCACGTTCGCGTGGACTGATACAACCATTGTGGTTGCCACTCTTGATGCAGGTGATGCTC
ATGGGATCGGATACACTTATACAGACGCGTCTGCCGCAGCTTTAATTGAACGAATGTTGG
CGCCGGCCCTTGATGGCCGTAGTGCATTTGATATCGGTGGGGCTCTCGCGGCCCTGCGCC
GTGCAGTCCGCAATGTAGGACGGGGCGGTGTGGCTGCAACTGCGATTTCAGCCCTGGATG
CGGCTCTGTGGGATGCAAAAGCCAAATTATTGGGCCTTCCACTCGCGAAGCTGTTAGGTG
GGGTTCGTGACGCTGTCCCTGTATACGGATCGGGCGGTTTCACCTCGTATGACGAGCACG
CCCTGCGCGCACAGCTGGAAGGTTGGCGTGCTCAAGGTCTGCATGCATGTAAAATAAAAG
TGGGCAGCGCGCCGAGAGATGACCCCGCTCGTGTTCGTATCGCACGTGATGCTCTGGGTG
GTGGTGTAGCACTGTTTGTCGATGCCAACGGCGCTTTCGCACCGCATGATGCTCTGGCAT
TTGCGCAACGTATTGCCGAATGCGACGTGCGTTGGTTCGAGGAACCAGTTTCTGCGGACG
ATATAGACGGCTTGCGCTTTGTGCGCGAGAGACTTGCCGCGGGTGTAGATGTCGCCGCTG
GTGAATACGGGTATACATTAGACGATTTCCGGGTTTTATTGGCGAGTGGCGCCCTTGACG
TGCTCCAGGCGGATGCAACACGCTGTGGTGGTGTTTCAGGCTTTTTAGAAGCCGCGACCT
TGGCCGATGCGTTTCACCGAGACCTTTCCGCTCACTGCGCACCTGCTCTGCATCGTCATG
TCTGTTGCGCCGCACCGCGTGTACGTCATGTTGAATGGTTCCACGATCACGCGCGTATCG
AGCGTATGTTATTTGACGGAGCCCCCACGCCTCGTGACGGTGCTATTGCGCCAGATATGT
CGCGCCCTGGTCTGGGTATCGAACTGAAAGCACAAGACGCGGCAAGATTTGCTGTTGAAG
GGCATCATCACCATCACCACTGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCA
CTCGAGCACCACCA

Protein sequence predicted based on nucleotide sequence

KPFSEPIIRCAKARAYRIPTDRPEADGTFAWTDTTIVVATLDAGDAHGIGYTYTDASAAA
LIERMLAPALDGRSAFDIGGALAALRRAVRNVGRGGVAATAISALDAALWDAKAKLLGLP
LAKLLGGVRDAVPVYGSGGFTSYDEHALRAQLEGWRAQGLHACKIKVGSAPRDDPARVRI
ARDALGGGVALFVDANGAFAPHDALAFAQRIAECDVRWFEEPVSADDIDGLRFVRERLAA
GVDVAAGEYGYTLDDFRVLLASGALDVLQADATRCGGVSGFLEAATLADAFHRDLSAHCA
PALHRHVCCAAPRVRHVEWFHDHARIERMLFDGAPTPRDGAIAPDMSRPGLGIELKAQDA
ARFAVEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a45c,A15A;g120t,T40T;
g123t,L41L;c126t,D42D;
t408a,G136G;c411g,S137S;
c514a,c516a,R172R;g756a,T252T;
c757t,c759a,L253L;g1011t,P337P;
t1017c,D339D
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-9282h
Recommended expression in:
Not Applicable