envZ (Idiomarina loihiensis L2TR) in pNYCOMPS-LIC-FH10T+ (N term)

Explanation of Terms

Gene:
Gene Symbol:  envZ
Gene Name:  osmolarity sensor protein
Original Clone ID:
GE.541.1.23_GO.13363
Keyword:
None
Species:
Idiomarina loihiensis L2TR
Type:
cDNA
Vector Name:
pNYCOMPS-LIC-FH10T+ (N term)
Format: 
CLOSED
Source:
New York Consortium on Membrane Protein Structure
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York Consortium on Membrane Protein Structure
IlCD00429930
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1311nts
Open reading frame: 48 to 1358

ATCACCATCACCACCATCACGAAAACCTGTATTTTCAATCCTACGTAATGAAACTTTTTC
CTAAGTCAGCGTTTGCCCGCACGGTTGCGCTGCTGGCTCTGGTGTTGCTTATTAACCAGC
TCGTTTCTTACGTGATGGTAAGCAATTATGTTGTCAGACCCTCTATAGAACAAATTAATG
AGCTGGTTGCTAAACACATCCGCACCATGTTTATCCCCCGTCAGCATTTAGCTGAAACCG
AGCGCGAGCTCATGACCAGTTATACTGAAGCGACCGGCATTGAAGTCTTTAATGATGAAG
CCGCTGAAAAGCAGGGCTTAGGTAACTCAACCTATTATGGTTATTTATCGGATGACATGA
GTGATATGCTGGGTGGTGAAGCGCAAGTACGGGTAGCTCAGGGCAATAAATTCTATTTGT
GGGTAAAACCTCCGCGTGCGCCTGACTTCTGGATACGCGTGCCCCTGGATAGTATGCAGC
AGGGCCGCTTCTCGCCATTGGTGATGTACCTGGTACTCATTGGTTTGCTGAGCGTGTTCG
GCGGTATGCTATTTGCCAACTGGTTAAACCGACCGTTAAAAGCTCTGGAGGACTCCGCCC
GAAGAGTTGGACGGGGAGAGTTCCCTGAGCAGCTGTCTGAAAAAGGGGCGACCGAAATTG
TGGCGGTGACTCGCGCTTTTAATCAAATGTCGCGCGGTATTCGCGAGTTGGAAAATGACC
GCAACCTTTTAATGGCCGGTGTTTCTCACGACTTAAGAACCCCTCTTACTCGCATTCGTC
TGGCTGCGGAAATGATGCCGCAGGGCGAAGACTGCCTGGCTCAGGGCATTATTGAAGATA
TAGACGACATGAACGACATTATTGATCAGTTTATTGATTACGTTCGTCTTGATCAGCAGG
AGTCGAGCAGCAGAGAGTCGCTGAATGAGCTGATAGAGCAAACTGTGGCGAATGTTCCGG
ATAACTGGCAAAAGCAGTTTGAGCTGGATTTAGGCAAGTTGCCCAGAGTCTGGTTACGGC
CCTTGTCTATGAAGCGAGTCATTATTAATTTGCTGGAAAATGCCGAACGTTACGGGAAAC
ATCAAGTGGTGGTCAGCTCCGGGCATGATAAAAAGCAAAAACGTGTTTGGTTTAGAGTAA
CGGATGACGGCCCGGGCATTCCCGAAGAGCAGCAGGCCACATTATTTGAGCCTTTTACTC
AGGGGGATAAAGCGCGTGGTGGCGTTGGGTCCGGTCTGGGGCTGGCTATTATTAAGCGCA
TTGTCGACCGGCACGACGGCAGTATAAAACTGCAAAACCGCCCGGAAGGCGGCTTGTGCG
TGCGGATTGAACTGCCGCTGAATCAGCAACGGCAGTAA

Protein sequence predicted based on nucleotide sequence

MKLFPKSAFARTVALLALVLLINQLVSYVMVSNYVVRPSIEQINELVAKHIRTMFIPRQH
LAETERELMTSYTEATGIEVFNDEAAEKQGLGNSTYYGYLSDDMSDMLGGEAQVRVAQGN
KFYLWVKPPRAPDFWIRVPLDSMQQGRFSPLVMYLVLIGLLSVFGGMLFANWLNRPLKAL
EDSARRVGRGEFPEQLSEKGATEIVAVTRAFNQMSRGIRELENDRNLLMAGVSHDLRTPL
TRIRLAAEMMPQGEDCLAQGIIEDIDDMNDIIDQFIDYVRLDQQESSSRESLNELIEQTV
ANVPDNWQKQFELDLGKLPRVWLRPLSMKRVIINLLENAERYGKHQVVVSSGHDKKQKRV
WFRVTDDGPGIPEEQQATLFEPFTQGDKARGGVGSGLGLAIIKRIVDRHDGSIKLQNRPE
GGLCVRIELPLNQQRQ*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinExpressed: Tested_Not_Found
ProteinPurified: Tested_Not_Purified
Find experimental protocols in GO.13363
Recommended expression in:
Not Applicable