HBLA_BACCE (Bacillus cereus) in pSGX4

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
10120c2BSt5p1
Keyword:
full-length cds
Species:
Bacillus cereus
Type:
cDNA
Vector Name:
pSGX4
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
PMID: 18175317
Title: X-ray crystal structure of the B component of Hemolysin BL from Bacillus cereus
Authors:
PSI
New York SGX Research Center for Structural Genomics
BcCD00430220
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 966nts
Open reading frame: 51 to 1016

ACAATGGAGATACGGCTCTTTCTGCAAATGAAGCGAGAATGAAAGAGACCTTGCAAAAGG
CTGGATTATTTGCAAAATCTATGAATGCCTATTCTTATATGTTAATTAAGAATCCTGATG
TGAATTTTGAGGGAATTACCATTAATGGATATGTAGATTTACCTGGTAGAATCGTACAAG
ATCAAAAGAATGCAAGGGCACATGCTGTTACTTGGGATACGAAAGTAAAAAAACAGCTTT
TAGATACATTGAATGGTATTGTTGAATACGATACAACATTTGACAATTATTATGAAACAA
TGGTAGAAGCGATTAATACAGGGGATGGAGAAACTTTAAAAGAAGGGATTACAGATTTGC
GAGGTGAAATTCAACAAAATCAAAAGTATGCACAACAACTAATAGAAGAATTAACTAAAT
TAAGAGACTCTATTGGACATGATGTTAGAGCTTTTGGAAGTAATAAAGAGCTCTTGCAGT
CAATTTTAAAAAATCAAGGTGCAGATGTTGATGCCGATCAAAAGCGTCTAGAAGAAGTAT
TAGGATCAGTAAACTATTATAAACAATTAGAATCTGATGGGTTTAATGTAATGAAGGGTG
CTATTTTGGGTCTACCAATAATTGGCGGTATTATAGTCGGAGTAGCAAGGGATAATTTAG
GTAAGTTAGAGCCTTTATTAGCAGAATTACGTCAGACCGTGGATTATAAAGTAACCTTAA
ATCGTGTAGTTGGAGTTGCTTACAGTAATATTAATGAAATGCACAAGGCGCTTGATGATG
CTATTAACGCTCTTACTTATATGTCCACGCAGTGGCATGATTTAGATTCTCAATATTCGG
GCGTTCTAGGGCATATTGAGAATGCAGCTCAAAAAGCCGATCAAAATAAATTTAAATTCT
TAAAGCCTAATTTAAATGCAGCGAAAGACAGTTGGAAAACATTACGAACAGATGCTGTTA
CATTAAAAGAAGGAATAGAGGAATTAAAAGTGGAAACTGTTACTCCACAAAAATAA

Protein sequence predicted based on nucleotide sequence

LQKAGLFAKSMNAYSYMLIKNPDVNFEGITINGYVDLPGRIVQDQKNARAHAVTWDTKVK
KQLLDTLNGIVEYDTTFDNYYETMVEAINTGDGETLKEGITDLRGEIQQNQKYAQQLIEE
LTKLRDSIGHDVRAFGSNKELLQSILKNQGADVDADQKRLEEVLGSVNYYKQLESDGFNV
MKGAILGLPIIGGIIVGVARDNLGKLEPLLAELRQTVDYKVTLNRVVGVAYSNINEMHKA
LDDAINALTYMSTQWHDLDSQYSGVLGHIENAAQKADQNKFKFLKPNLNAAKDSWKTLRT
DAVTLKEGIEELKVETVTPQK*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
K50R; T122N; I291M; D292H

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-10120c
Recommended expression in:
Not Applicable