DNASU Plasmid | Q7CWL6 (A. fabrum str. C58 )<br>In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol:

Gene Name:

Original Clone ID:

10456l2BCt1p1

Keyword:

full-length cds

Species:

Agrobacterium fabrum str. C58

Type:

cDNA

Vector Name:

pSGX3

Format:

CLOSED

Source:

New York SGX Research Center for Structural Genomics

Description:

None

Comments:

Linker not verified.

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
New York SGX Research Center for Structural Genomics

AtCD00430265

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1587nts

Open reading frame: 49 to 1635

GAGCCACGTCTGGAAGGCCCGCAGAAGCAGGCTGCTTCGGAGATAATTGAAGATGTGCGC
AAGGGTGGCTTTCATGTCTCGCTGATCGACGGCGTGACCGGGTCCGGCAAGACCGAGGTT
TATTTCGAGGCGGTGGCCGAAACGCTGCGACAAGGCAAGCAGGTCCTTATCCTGCTGCCG
GAAATCGCCCTGACGGCGGCGTTTCTTGAGCGTTTTCAGGATCGCTTCGGTGCGAAACCG
GCGGAATGGCATTCGGATCTGTCGCCGCGCATGCGCGAAAAGGTCTGGCGGCAGGCGGTG
ACGGGTGAGATAAAGGTGGTGGCCGGCGCGCGCTCGGCGCTTTTCCTGCCCTTCGACAAT
CTAGGCCTCATCATTGTCGATGAAGAGCACGACCCGGCCTACAAGCAGGAAGACCGCGTC
TTTTATAATGCCCGTGACATGGCTGTCGTGAGGGCGCGGATCGCCGAATTCCCAGTCGTG
CTGGTTTCGGCCACGCCGTCGGTGGAAAGCCAGGTCAATGGCAGTTCCGGGCGTTACAAC
ACCATTCATCTGCATACCCGCTTCGGCGATGCGGCGATGCCGGACCTGCATCTCGTCGAT
ATGCGCCGCCATCCGCCGGAAAGGGGAGGGTTTCTCTCGCCCGTCCTGCTGCGCGGCATC
GGCAAGACCATCGAAAAGGGCGAGCAGTCCCTGCTCTTTCTCAATCGCCGCGGTTATGCG
CCGCTGACGCTCTGCCGGGTTTGCGGTCACCGTTTCCAATGCCCACAATGTTCCAGCTGG
CTGGTGGAACACCGTTTCCGCAATCAATTGCAGTGCCACCAATGTGGCCACAACGAGCCG
ACGCCCGACCATTGCCCGGAATGCGGAACCTTCGATCATCTCGTGGCCTGTGGGCCGGGG
GTGGAGCGGATCGCCGAGGAGGTTGAGAAACATTTCCCCGAGGCCCGCACCATCGTGCTG
TCTTCCGATCTCATGGGCGTCAAGCGGCTGCGTCTGGAGCTTGAGGCGATCGTCAAGGGC
GAGGCGGATATCGTCATCGGCACGCAGCTCGTCGCCAAGGGACATAACTTCCCGCTGATG
ACGCTCGTCGGCATCGTCGATGCCGATCTCGGCCTTGCCAATGGCGATCCGCGCGCGGCG
GAGCGGACGTTCCAGTTGCTTTCGCAGGTGACCGGCCGTGCCGGACGAACGGGATTGAAG
AGTCACGGCCTGCTGCAGACCTACCAGCCGCAGCACCCGGTGATGCAAGCGATCGTCTCC
GGTGATGCCTCGGCCTTTTATGAAAGGGAGATCGTCGAGCGGGAAAAGGCCGTTCTGCCG
CCCTTCGGCCGCCTCGCTTCGATCATCGTCTCCGCCGATACGCGCGCGGAGGCGGAAACC
CATGCGCGTGGATTGCGAGCCGCGGCTCCGCAGGTCACCGGCATCATGCTTCTCGGCCCG
GCGGAAGCGCCGCTGGCACTGATCCGTGGCCGTCACCGTTTTCGTCTGCTGGTGCACGGG
CGGCGCAATTCCGACATGCAGTCTTTTCTTCGCACGCTTCTTGCGAGTGGCCCGAAGGAG
CGTGGCAGCGTGCATGTGCAGCTCGATATCGATCCGCAAAGTTTCCTTGAAGGGCATCAT
CACCATCACCACTGATCCGAATTCGAGCTCCGTCGAC

Protein sequence predicted based on nucleotide sequence

EDVRKGGFHVSLIDGVTGSGKTEVYFEAVAETLRQGKQVLILLPEIALTAAFLERFQDRF
GAKPAEWHSDLSPRMREKVWRQAVTGEIKVVAGARSALFLPFDNLGLIIVDEEHDPAYKQ
EDRVFYNARDMAVVRARIAEFPVVLVSATPSVESQVNGSSGRYNTIHLHTRFGDAAMPDL
HLVDMRRHPPERGGFLSPVLLRGIGKTIEKGEQSLLFLNRRGYAPLTLCRVCGHRFQCPQ
CSSWLVEHRFRNQLQCHQCGHNEPTPDHCPECGTFDHLVACGPGVERIAEEVEKHFPEAR
TIVLSSDLMGVKRLRLELEAIVKGEADIVIGTQLVAKGHNFPLMTLVGIVDADLGLANGD
PRAAERTFQLLSQVTGRAGRTGLKSHGLLQTYQPQHPVMQAIVSGDASAFYEREIVEREK
AVLPPFGRLASIIVSADTRAEAETHARGLRAAAPQVTGIMLLGPAEAPLALIRGRHRFRL
LVHGRRNSDMQSFLRTLLASGPKERGSVHVQLDIDPQSFLEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in NYSGXRC-10456l

Recommended expression in:

Not Applicable

Vector Name:

pSGX3

Synonyms:

pSGX-3, pSGX3_BC

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.

Comments:

Modified from pET26b from Novagen

Size (bp):

5339

Parent Vector:

None

Properties:

TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker

Author Name:

New York SGX Research Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCTTAAGGGCATCATCACCATCACCACTGATCCCACCACCACCACCACCACTGAGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGAT

Vector Features:

Type

Name

Description

Start

End

ATG start

start

initiation start (Met, Ser, Leu)

5072

5079

TOPO recognition site

TOPO

TOPO cloning site

5076

5085

bacterial origin

ori

pBR322-type bacterial origin of replication

1421

2103

gene fragment

ROP

2527

2703

promoter

T7 promoter

4983

5003

repressor protein gene

LacI

LacI CDS

3515

4531

selectable marker

KanR

kanamycin resistance gene

563

1375

tag

His

6xHis tag

5086

5103

trxn regulatory element

lac operator

5003

5025

trxn termination sequence

T7 term

T7 terminator

5231

5307

Gene

GenBank Accession :

Q7CWL6_AGRT5

Target GenBank:

None

Protein

SMART domain :

HELICc : helicase superfamily c-terminal domain

SMART domain :

DEXDc : DEAD-like helicases superfamily

Clone

Cloning Information :

495419

HIP Master Clone ID :

409099

Original Clone ID :

10456l2BCt1p1

Experimental

TargetTrack - Experimental data :

NYSGXRC-10456l

Q7CWL6 (A. fabrum str. C58 )In pSGX3 (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Protein

Clone

Experimental

Q7CWL6 (A. fabrum str. C58 )
In pSGX3 (His-tagged bacterial expression vector)