DP0251 (D. psychrophila )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  DP0251
Gene Name:  N-acylamino acid racemase (MenC)
Original Clone ID:
9393a1BCt1p1
Keyword:
full-length cds
Species:
Desulfotalea psychrophila
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
DpCD00430270
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1128nts
Open reading frame: 1 to 1128

AGCGGCATGGAACTGTCTTATCGTCGCAGTGATTTAATTTTTAAACGTCCGGCGGGTACC
TCACGGGGGGTGTTGACGTCCAAGCCAACTTGGTTCGTTCGCCTTGACATCGATGGACAT
GGCGGTCAGGGGGAGGTCTCGCTCATACCTGGACTGAGCCTGGACCCCGAAGAGCAAATT
GGCAGAGAATTGGACTTATTAGCGCGCAGACTGCGGGCAGAAGAGCCGATCCGTTTAAGA
CAGTTTCTGGCTGAACGCGGTGGCGCGGACTTCTCTGATTACAGAAGTGTATTAACAGAT
ATTGCCGGTATCCTGGACTCATGGCAAGTGTCCACCGATGGGCGATTTCCAGCATTGCGT
TTCGCTCTTGAGATGGCGCTGCTTGATCTGCTGTCGGGAGGCCGCCAGGAATGGTTTGCC
AGCGATTTCACGCGGGGTGAAAAACGTATACCTGTTAATGGCTTGATTTGGATGGGTGAG
GCAGCTTTTATGCAGGAACAAATCGAGGCGAAACTTGCCGAAGGGTATGGATGCCTGAAG
TTAAAAATTGGCGCAATAGACTTCGATAAAGAATGTGCTCTGCTCGCGGGTATCCGCGAG
TCTTTTAGTCCGCAGCAACTGGAAATTCGTGTCGATGCCAACGGGGCATTTTCACCCGCG
AATGCTCCGCAGCGCTTGAAGAGACTTTCCCAGTTCCACCTGCATTCGATCGAGCAACCA
ATTCGACAGCACCAATGGAGCGAAATGGCCGCATTATGCGCGAACTCTCCTCTGGCTATA
GCCCTCGACGAAGAGTTGATCGGCCTGGGTGCAGAACAGCGGAGTGCGATGCTTGATGCT
ATTCGTCCGCAGTACATAATCTTAAAACCCTCACTGCTGGGAGGCTTTCATTATGCCGGT
CAATGGATTGAACTTGCACGCGAGCGTGGGATTGGCTTCTGGATCACTTCCGCGTTGGAA
AGCAACTTGGGTTTAGCCGCAATTGCGCAGTGGACAGCTCTGTACCAACCGACCATGCCA
CAGGGACTGGGCACGGGTCAGCTCTATACCAACAATTTGCCTTCGAATCTCGCCGTAGAT
GGGGGATTACTTGGCGTGTCTGAAGGGCATCATCACCATCACCACTGATCCGAATTCGAG
CTCCGTCGAC

Protein sequence predicted based on nucleotide sequence

SGMELSYRRSDLIFKRPAGTSRGVLTSKPTWFVRLDIDGHGGQGEVSLIPGLSLDPEEQI
GRELDLLARRLRAEEPIRLRQFLAERGGADFSDYRSVLTDIAGILDSWQVSTDGRFPALR
FALEMALLDLLSGGRQEWFASDFTRGEKRIPVNGLIWMGEAAFMQEQIEAKLAEGYGCLK
LKIGAIDFDKECALLAGIRESFSPQQLEIRVDANGAFSPANAPQRLKRLSQFHLHSIEQP
IRQHQWSEMAALCANSPLAIALDEELIGLGAEQRSAMLDAIRPQYIILKPSLLGGFHYAG
QWIELARERGIGFWITSALESNLGLAAIAQWTALYQPTMPQGLGTGQLYTNNLPSNLAVD
GGLLGVSEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
del@1,3;a192g,L64L;
t195c,D65D;g198a,L66L;
c199t,t201a,L67L;c204g,A68A;
a207c,R69R;c208a,t210a,R70R;
c216g,R72R;g234t,R78R;
c235t,c237a,L79L;c238a,t240a,R80R;
c384t,L128L;c387t,D129D;
t388c,a390g,L130L;c915t,L305L;
del@966,1;ins@969,1;
c1068t,N356N;t1069c,a1071c,L357L
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-9393a
Recommended expression in:
Not Applicable