APE_1865.1 (A. pernix K1 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  APE_1865.1
Gene Name:  PflA-like protein
Original Clone ID:
APC61963_SG173_pMCSG19
Keyword:
full length
Species:
Aeropyrum pernix K1
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Midwest Center for Structural Genomics
ApCD00432620
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1101nts
Open reading frame: 2 to 1102

CATGGGTAGTCTAAGGCTTACGGAGAGACCGCACGTAAGGAAGGCTAGGCTTTGGGTAAA
ACTTAGTGACGGTAAGGAGGGGTGGGTGAGGTGCGATCTCTGCCAGAGGAGGTGTGTTAT
AGCTCCTGGAAAGTATGGGGCTTGTGGTGTTAGGAAGAACATAGGAGGGGAACTCTACAC
TCTGGTCTATGGCCTTCTGAGCGCTAGCAATATAGACCCTATTGAGAAGAAGCCTCTACT
ACACTTTTACCCGGGCTCGACAGTTCTGAGTATAAGCACCGTTGGGTGCAACTTCTTCTG
CCAGTTCTGCCAGAACTTCGAGATAAGCCAGTCCCGGCTTGAGAAGGGTCTTTACGGTGT
CTATAGGACGCCTGAGGAGGTTGTGAGGCTAGCCTTAGTCTATGGAGCCGACGGCATCAC
CTATACCTACAACGAGCCCACAATATTCTTTGAAACTGTTTACGACGTGGCAAAGGAAGC
TAAAAAGCATGGCCTCTTCAACACTATGGTGACCAACGGCTACATGACCCCCGAAGCTGT
TGATGAGCTAGGAAGCCTCATAGACGCTGCTACTGTCGATTTCAAGGGAGGAGGCGACCC
CGAGTTCTACAGGAAGATAATGTTCGTGCCGGACCCCGAACCCATATACGAGGCGATACT
TGCTATGAAGGATAAAGGCTGGTGGATAGAGATAACAAACCTGGTTGTCCCTATCCTCGG
CGATAAGCCTGAGTATGTTAGGCGTATGGCCAGATGGATAGTTGAAAACCTTGGACCGGA
GGTGCCCTTCCACCTGCTGAGATTCCATCCAGACTACAAGCTAAGGCACGTACCCCACAC
TCCAGTTAAGACCCTCGAGAAGCTGGCCAGAATAGCTATGGAAGAGGGCCTCAAACATGT
TTATATAGGCAACGTGTGGGGCCACCCGCTCGAGGACACGTACTGTCCCAAGTGTGGATA
CAAAGTTGTTGACAGGGAGGGTTTCGCAATCGTAGAATGGCGGCTAACAAGCGACTTCAG
ATGCCCACGCTGCGGGTATAAGCTCAATTTCCGCGGCGAGTACACGCCACGTAACATAAA
CTATCCCATGCCAGTGATGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGT
CGACAAGCTTGCG

Protein sequence predicted based on nucleotide sequence

MGSLRLTERPHVRKARLWVKLSDGKEGWVRCDLCQRRCVIAPGKYGACGVRKNIGGELYT
LVYGLLSASNIDPIEKKPLLHFYPGSTVLSISTVGCNFFCQFCQNFEISQSRLEKGLYGV
YRTPEEVVRLALVYGADGITYTYNEPTIFFETVYDVAKEAKKHGLFNTMVTNGYMTPEAV
DELGSLIDAATVDFKGGGDPEFYRKIMFVPDPEPIYEAILAMKDKGWWIEITNLVVPILG
DKPEYVRRMARWIVENLGPEVPFHLLRFHPDYKLRHVPHTPVKTLEKLARIAMEEGLKHV
YIGNVWGHPLEDTYCPKCGYKVVDREGFAIVEWRLTSDFRCPRCGYKLNFRGEYTPRNIN
YPMPVM*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in APC61963
Recommended expression in:
Not Applicable