nirJ-2 (A. fulgidus DSM 4304 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  nirJ-2
Gene Name:  heme biosynthesis protein (nirJ-2)
Original Clone ID:
APC61994_SG173_pMCSG19
Keyword:
full length
Species:
Archaeoglobus fulgidus DSM 4304
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Midwest Center for Structural Genomics
AfCD00432623
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1407nts
Open reading frame: 32 to 1438

GGGTCCGAGAACTGTACTTCCATCCAATGCCGTGGACATGAACGTGGAAATTTTTGCTGG
CATTGTCAACAACCCTTTAACCAAAAAAGCACTCAGAAATCTTTCAGGTTCATGCAAAAC
CTGTGGAGACAAAAGAATAAAGATTGCCATAGATTACGCTCTCGGCTACCGCAGTGATGC
CTGTTGGAAGTGCAGGGCTGCTGCCAAAGTTGTCAAGAAGGTTATTGAGGCTGGCGGAAG
GGCATTCAATGTTGACATGAATGAAATAAGGGAGAAGTTCAGAGATGCTTACTGGAGAAA
GGGGTTGGCGAGCGTTATCAAAGGAATAGCCCACTTCGGAGTCAGAAAGCCCTTCGTTCC
CGGCGCGCCCTTTCAGGTTGTGTGGGACATCACATATTCCTGCAACCTCCGCTGCAAGCA
CTGCTACGCAACAGCAGGCAAACCACTACAGGATGAGCTAACAACCGATGAAGCTCTCGA
AACAATCGACAAGCTGGACAGGCTTGGCGTCACAATCATCGCCTTTTCCGGAGGAGAACC
GCTCGTAAGAAGGGACATCTTCGAGCTGACGAGATACGCTGCCGAGAAGGGGATTTACGT
TGCCATAGCAACCAACGGAACCCTAATAACAGAAGAAATAGCTAAAAGGATGAAGGAGAA
TGGAGTTGGCTACGTGCAGATCAGCCTTGATGGGATGAAAGAGACTCATGAGGCTTTCAG
GGGGATAAGGGGTTGCTTCGACAAAACAGTTGAGGGGATCAGGAATGCTGTCAAGACGGG
ATTGTTCGTTAACGTTTCCATGACGGTGACCAGATACAACTACCACGAGGTGCCCAAAGT
GGTCGAGCTCTGCGAAAAGCTGGGAGTGAACTGGTTCATGCACTACAACTTCATCCCAAC
AGGAAGAGGTAGAGAGATAGTGGAGGCGGACATAACTCCCGAGCAGAGGGAGGAGCTGCT
GAAATGGCTCTACGAGAAAAACTACAGCTCGAACATTTCCCTGCTCTCAACCGCCCCCCA
GTTTGCCCGCGTTGCACTGCAGTGTCAGGGAGAGATTGTGCCGACGCACTTCTACAATGT
AAACGCTGGTGAGAGGCTGAAGGAGCTGGCGGAGTTCATTGGTGGTTGTGGGGCTGGGAG
GTTCTACTTCGCCATAAAGGCGAACGGCGACATTCAGCCCTGCGTCTTCTTCCCGCTTAA
GGTTGGTAATGTGAGGGAGGATGATCTGGAGGAGCTTTGGCTTCACAACCACGTTTTCGA
GGATTTGAGGGATAAGGATATTCTTGAGGGCTGTGGAAGCTGTGGATACAGGTACGTGTG
TGGAGGCTGCAGAGCTAGGGCCTACAACTACTTTGGAGACTACACTAAGCCCGATCCCGG
GTGTGTGAATAATAGAAGGTGGTGGGAGAGGATTAGTAAAATTGAGGTTAGGGCTTAACA
TTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTC

Protein sequence predicted based on nucleotide sequence

VDMNVEIFAGIVNNPLTKKALRNLSGSCKTCGDKRIKIAIDYALGYRSDACWKCRAAAKV
VKKVIEAGGRAFNVDMNEIREKFRDAYWRKGLASVIKGIAHFGVRKPFVPGAPFQVVWDI
TYSCNLRCKHCYATAGKPLQDELTTDEALETIDKLDRLGVTIIAFSGGEPLVRRDIFELT
RYAAEKGIYVAIATNGTLITEEIAKRMKENGVGYVQISLDGMKETHEAFRGIRGCFDKTV
EGIRNAVKTGLFVNVSMTVTRYNYHEVPKVVELCEKLGVNWFMHYNFIPTGRGREIVEAD
ITPEQREELLKWLYEKNYSSNISLLSTAPQFARVALQCQGEIVPTHFYNVNAGERLKELA
EFIGGCGAGRFYFAIKANGDIQPCVFFPLKVGNVREDDLEELWLHNHVFEDLRDKDILEG
CGSCGYRYVCGGCRARAYNYFGDYTKPDPGCVNNRRWWERISKIEVRA*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in APC61994
Recommended expression in:
Not Applicable