SAV0321 (S. aureus subsp. aureus Mu50 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  SAV0321
Gene Name:  hypothetical protein
Original Clone ID:
APC61011_SG123_pMCSG19
Keyword:
full length
Species:
Staphylococcus aureus subsp. aureus Mu50
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Midwest Center for Structural Genomics
SaCD00432683
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 828nts
Open reading frame: 19 to 846

GTACTTCCATCCAATGCCGTGACTGAAATTAAATATAAAGTAATTACTAAAGATGCATTT
GCATTACCTTACACAATTATTAAAGCAAAAAATCAACCAACAAAAGGCGCTATTGTCTAC
ATCCACGGTGGTGGATTAATGTTCGGTAAAGCTAACGATTTATCCCCACAGTATATCGAT
ATCCTTACGGAACATTACGATTTAATTCAACTAAGTTATCGTTTATTACCAGAAGTAAGT
CTCGATTGCATTATTGAGGATGTCTACGCATCATTCGATGCGATTCAATCACAATATTCT
AATTGCCCTATTTTTACCTTTGGCAGATCATCAGGTGCATATTTAAGTCTATTAATTGCA
CGAGACAGAGATATTGATGGTGTTATTGATTTTTATGGTTATAGTCGCATCAACACTGAA
CCTTTTAAAACAACTAATAGTTATTACGCAAAAATAGCTCAAAGCGTAAACGAAACAATG
ATTGCACAACTCACTTCACCAACACCGGTTGTTCAAGATCAAATCGCACAACGTTTTTTA
ATCTACGTTTATGCACGTGGCACAGGTAAATGGATAAATATGATTAACATTGCTGATTAT
ACTAATTCAAAATATAATATTGCACCAGATGAACTTAAAACACTTCCACCTGTTTTCATT
GCGCATTGTAATGGCGATTATGATGTTCCCGTTGAGGAAAGCGAACATATCATGAATCAT
GTTCCACATTCAACATTTGAACGCGTGAATAAAAACGAGCATGATTTTGATCGTAGACCT
AATGACGAAGCAATCACTATTTATCGTAAAGTTGTTGATTTCTTAAACGCTATAACAATG
GTGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCG

Protein sequence predicted based on nucleotide sequence

VTEIKYKVITKDAFALPYTIIKAKNQPTKGAIVYIHGGGLMFGKANDLSPQYIDILTEHY
DLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFGRSSGAYLSLLIARDRDID
GVIDFYGYSRINTEPFKTTNSYYAKIAQSVNETMIAQLTSPTPVVQDQIAQRFLIYVYAR
GTGKWINMINIADYTNSKYNIAPDELKTLPPVFIAHCNGDYDVPVEESEHIMNHVPHSTF
ERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMV*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t92c,V31A;a448g,I150V;
g586a,D196N
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in APC61011
Recommended expression in:
Not Applicable