uxaA (Y. enterocolitica subsp. enterocolitica 8081 )
In pMCSG19B (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  uxaA
Gene Name:  altronate hydrolase
Original Clone ID:
APC38775_SG255_pMCSG19B
Keyword:
full length
Species:
Yersinia enterocolitica subsp. enterocolitica 8081
Type:
cDNA
Vector Name:
pMCSG19B
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Brian Feldmann
PSI
Midwest Center for Structural Genomics
YeCD00432845
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1491nts
Open reading frame: 48 to 1538

AAAACTCGGCGGGATTGGGTACGAAACCTGTACTTCAATCCAATGCCATGCAAAGTATTA
TAAAAATTCATCCGTTGGATAATGTGGCTGTCGCACTGCAAGATTTAGCTGCTGATGATG
TTCTCGAAATTGGAGAATTCAGTGTTAAATTGGCTCAACCAGTAGCGCGTGGTCATAAAT
TTGCTTTAACGGCCATTGCACCGGGGCAAATGATCGTTAAGTATGGTTTGCCGATCGGCC
ATGCATTAACCCTGATCCAGCCCGGCGAACATATTCATTCGCAAAATGCTAAAACTAACC
TCAGCGATCTGGATGAGTATCAGTATCAGCCAGAATTCAGCGAGTTACCGCCACAAATGG
CAGATCGCGAAGTGCAGATCTATCGCCGGGCCAATGGTGATGTGGCGGTGCGCAACGAAC
TGTGGATTATTCCAACGGTAGGTTGCGTGAATGGGATCGCCCGTCAGATCCAACAACGTT
TTCTGAAAGAAACACAAGACGCGGAAGGTATTGATGGGGTCTATCTGTTCAGCCATCCAT
TTGGTTGTTCACAATTGGGTCAGGATCACGAAAATACCCGCACCATGCTGCAAAACATGG
TGCGCCACCCCAATGCTGGTGCGGTGCTGGTCATTGGTTTGGGCTGTGAAAACAACCAGG
TAGACGTTTTCCAAACCACTTTGGGGCCGGTTGATGAAGATCGCGTGCACTTTATGGTCT
GTCAGCAGCAGGATGACGAAGTTGAAGCCGGTCTTGAGCATCTGCATGCTTTGTATCAGG
TCATGCGCCATGACCAACGGGTTGCGGGTAAGTTGAGTGAACTCAAGTTTGGTCTGGAAT
GCGGGGGTTCTGATGGGTTATCTGGTATCACCGCTAACCCATTACTGGGCCGCTTCTCCG
ACTATGTGATTGCCAATGGCGGAACCTCTGTTCTGACCGAAGTGCCGGAAATGTTCGGCG
CTGAGCGGATTCTGATGAGCCGCTGCCGTGATGAAGCGACGTTTGAAAAGACTGTCAGTA
TGGTGAATGACTTCAAACAGTACTTTATTGCGCACAACCAGCCGATTTATGAAAACCCAT
CTCCGGGCAATAAAGCAGGCGGTATCACTACGCTGGAAGAGAAATCACTGGGTTGCACCC
AAAAAGCGGGCCAGAGTAAAGTGGTTGATGTGTTGAAGTATGGTGAGCGTTTACAGCACC
CTGGGCTGAATTTACTCAGTGCGCCGGGTAATGATGCAGTAGCAACCAGTGCATTAGCGG
GCGCCGGTTGTCATATGGTGTTGTTCAGTACCGGACGTGGTACACCTTATGGCGGCTTTG
TACCGACAGTTAAGCTGGCAACTAATAGCGAATTGGCCGCTAAAAAACCCCACTGGATTG
ATTTCGATGCCGGTAAACTGATTCACGGCACGTCAATGGATGATTTGCTGACAGAGTTTG
TTGATTTGATTGTCGACATTGCCAACGGCAAACCAGCGCGCAATGAAGTGAATGACTTCC
GTGAACTGGCAATCTTTAAAAGCGGTGTAACCTTGTAACATTGGAAGTGGATAACGGATC
CGAATTCGAGCTCCGTCGAC

Protein sequence predicted based on nucleotide sequence

MQSIIKIHPLDNVAVALQDLAADDVLEIGEFSVKLAQPVARGHKFALTAIAPGQMIVKYG
LPIGHALTLIQPGEHIHSQNAKTNLSDLDEYQYQPEFSELPPQMADREVQIYRRANGDVA
VRNELWIIPTVGCVNGIARQIQQRFLKETQDAEGIDGVYLFSHPFGCSQLGQDHENTRTM
LQNMVRHPNAGAVLVIGLGCENNQVDVFQTTLGPVDEDRVHFMVCQQQDDEVEAGLEHLH
ALYQVMRHDQRVAGKLSELKFGLECGGSDGLSGITANPLLGRFSDYVIANGGTSVLTEVP
EMFGAERILMSRCRDEATFEKTVSMVNDFKQYFIAHNQPIYENPSPGNKAGGITTLEEKS
LGCTQKAGQSKVVDVLKYGERLQHPGLNLLSAPGNDAVATSALAGAGCHMVLFSTGRGTP
YGGFVPTVKLATNSELAAKKPHWIDFDAGKLIHGTSMDDLLTEFVDLIVDIANGKPARNE
VNDFRELAIFKSGVTL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c978g,V326V;g1395t,V465V
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in APC38775
Recommended expression in:
Not Applicable