tyrA (H. influenzae ) in pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  tyrA
Gene Name:  bifunctional chorismate mutase/prephenate dehydrogenase
Original Clone ID:
23561
Keyword:
full-length cds
Species:
Haemophilus influenzae
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
HiCD00432951
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1134nts
Open reading frame: 51 to 1184

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGAGTTTTA
TGGAAGCCCTTAAAGATTTACGTTCTGAAATTGATTCGCTTGATCGCGAACTTATCCAAC
TTTTTGCTAAACGTCTTGAGTTGGTTTCTCAAGTCGGTAAAGTGAAACATCAACATGGAT
TACCTATTTATGCGCCAGAACGTGAAATAGCAATGCTCCAAGCACGTCGTTTAGAAGCTG
AAAAAGCAGGCATTTCAGCCGATTTAATTGAAGATGTTCTACGCCGTTTTATGCGTGAAT
CCTATGCCAATGAAAACCAATTTGGTTTTAAAACTATCAATTCTGATATTCACAAAATTG
TTATTGTGGGCGGTTATGGTAAATTAGGCGGCTTATTTGCCCGTTATTTACGTGCATCTG
GCTATCCAATTTCTATTTTAGATCGCGAAGATTGGGCGGTGGCTGAAAGTATTTTAGCGA
ATGCTGATGTCGTGATCGTTTCCGTCCCTATTAATCTCACCTTAGAAACAATTGAACGCT
TAAAACCTTATTTAACGGAAAACATGCTACTTGCAGATTTAACCTCTGTTAAGCGTGAAC
CGCTAGCGAAAATGCTTGAAGTTCATACTGGTGCTGTTTTAGGTTTACATCCAATGTTTG
GTGCAGATATTGCAAGTATGGCAAAACAAGTGGTTGTGCGTTGTGATGGACGTTTTCCTG
AACGTTATGAATGGTTACTTGAGCAAATTCAAATTTGGGGTGCAAAAATTTATCAAACCA
ATGCCACAGAACACGATCATAATATGACTTATATACAAGCCTTGCGCCATTTTTCGACTT
TTGCGAATGGTTTACACCTTTCCAAACAGCCCATTAATCTCGCTAATTTATTGGCACTTT
CTTCCCCTATTTATCGGTTAGAACTTGCGATGATAGGTCGTTTATTTGCGCAAGATGCAG
AGCTTTACGCAGATATTATTATGGATAAGTCAGAAAATTTAGCGGTAATTGAAACGCTAA
AACAAACTTACGACGAAGCCTTAACTTTCTTTGAAAATAATGATCGTCAAGGTTTTATTG
ATGCTTTCCACAAGGTGCGTGACTGGTTTGGCGATTATTCCGAACAATTTTTAAAAGAAA
GCCGTCAGCTATTGCAACAGGCAAATGATTTGAAACAAGGGTAACGCGACTTAATTAACT
CGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGG

Protein sequence predicted based on nucleotide sequence

MSFMEALKDLRSEIDSLDRELIQLFAKRLELVSQVGKVKHQHGLPIYAPEREIAMLQARR
LEAEKAGISADLIEDVLRRFMRESYANENQFGFKTINSDIHKIVIVGGYGKLGGLFARYL
RASGYPISILDREDWAVAESILANADVVIVSVPINLTLETIERLKPYLTENMLLADLTSV
KREPLAKMLEVHTGAVLGLHPMFGADIASMAKQVVVRCDGRFPERYEWLLEQIQIWGAKI
YQTNATEHDHNMTYIQALRHFSTFANGLHLSKQPINLANLLALSSPIYRLELAMIGRLFA
QDAELYADIIMDKSENLAVIETLKQTYDEALTFFENNDRQGFIDAFHKVRDWFGDYSEQF
LKESRQLLQQANDLKQG*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g1a,V1M
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 361237
Recommended expression in:
Not Applicable