Gene:
Gene Symbol:
DR_A0331
Gene Name:
hypothetical protein
Original Clone ID:
2836c1TNt5b1
Keyword:
full-length cds
Species:
Deinococcus radiodurans
Type:
cDNA
Vector Name:
pSB2
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
DrCD00432989
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1491nts
Open reading frame: 51 to 1541
TATACATAATGGGCCATCATCATCATCATCACGGTGGTCATATGTCCCTTTCAAATGCTC
TAGTAAATCCGCGTGAGGCCCCGGCTCATGGCCGCTTTGTCCCGGCACCTATACTTGCCC
ACAGAAACTGCACGCAAGGAGCGCTGAATATGCTAGAGGCCAAGCTGAAACCCCACCGTG
AATACCTGCTGGCGCAGTCCGCCGGCCAGAAACTCTTTCTGACCCTGACCGTGAAGCCCA
CCGCCGAGGCGCGGCAGGCGCGGCCCGACCTGAGCGTGGTGTTCGTGGTGGACACCTCCG
GCTCCATGCGCGAGGTCGTGACCGAACCCACCGAGCGCACCGGGCGCACGACGCAGGTGG
ACGGTCAGGTCTACGAAGTCGTCAAGGGCGGCAAAAACAAGATGCAGCTGATGATCGAGG
CGCTGCGCGGCATCGTGACCTCCACCCTGATTCGGCCGGGCGACCGCCTCGCGCTGGTCA
AGTTCGACGACACCGCCGAGGTCCTTGTCCCATTCATCACCGCCAGCAATCAGGCGCAGC
TCGCCGCCGCCGTGGACAAGCTCGACTGGTACTCGGGCGGCACCCACATGGGCGCGGGAA
TGCGCGCCGGGGCCGGCCTGCTCACGGGCGAGCGCGGCAGCCGCCGCATGGTGCTGATCA
GCGACGGGCAGACCTTCGACGCGCCGCTGGTGGAAGACCAGATCGGCGCCCTGGCAGGGC
TGCAAGTGCCCGTGACGGTGGTGGCCGTGGGCGACGAGGTGAACGCCGACCTGCTCACCG
CCATCGCCGACCGCACGCAGGGGCAGCCGCTCGACATCGTGCCCGACACGCAAAATCCGC
AGCCGCCCGCCATCCGCGCTTCCGAGTTGCCTGCCGCGCTGCTCGGCGACCTGCAAAAAG
CCGCCGCCGAAGTGGTCACGGGCGTGGCCCTCTCGGTCCGCACCGTCAAAGACGTGGTCC
TCGAGCGCGTCACCCGCGTGCAGCCCACCCAGACCGAGGTGGCGCTGCGGGCCGACGGGC
CTCTCCCGCTCGGCAACGTGGACGCGGGCAGCGGGGCGACCTTCGTGCTGGAGTTCACCC
TGCCTGCCCGCCCGGCGACCAAGATGCGGCTCGCGCAGCTCGGCCTGACCTACGAGGTGC
CCGGCGCGAACTACCGGGGCGAGATTCCCCCGCTCGACGTGGTGGTGGAATTTACCGGCG
AGGAAGCCCTGGCCGCCCGGATAGACCCCGAGGTCATGGGCTGGGTGCAGCAGCGCAACA
TCGAGGGGCTGGTCGCGCAGGCCACCCGCGAGGCGAACCAGAACCCCGAACAGGCGGCCA
AGACGCTTGAGCAGGCCCGCGCCATGACCCAGCGCCTCGGCAACGGCGCCATGACGCAGG
CACTCGACCGCGCCATCGGCGAACTCGGCAGCTCCAAGACCATCAGCCTCGGCACCGCCA
AGACGCTCAAAATCGGCGCCAAGACCCAGACCCTCAGCAGCGACGCCGCCCTGCCGTCCG
ATGAGGACATCCGCCGCATGACCGGAGAAGGGGGATCCTAA
Protein sequence predicted based on nucleotide sequence
SNALVNPREAPAHGRFVPAPILAHRNCTQGALNMLEAKLKPHREYLLAQSAGQKLFLTLT VKPTAEARQARPDLSVVFVVDTSGSMREVVTEPTERTGRTTQVDGQVYEVVKGGKNKMQL MIEALRGIVTSTLIRPGDRLALVKFDDTAEVLVPFITASNQAQLAAAVDKLDWYSGGTHM GAGMRAGAGLLTGERGSRRMVLISDGQTFDAPLVEDQIGALAGLQVPVTVVAVGDEVNAD LLTAIADRTQGQPLDIVPDTQNPQPPAIRASELPAALLGDLQKAAAEVVTGVALSVRTVK DVVLERVTRVQPTQTEVALRADGPLPLGNVDAGSGATFVLEFTLPARPATKMRLAQLGLT YEVPGANYRGEIPPLDVVVEFTGEEALAARIDPEVMGWVQQRNIEGLVAQATREANQNPE QAAKTLEQARAMTQRLGNGAMTQALDRAIGELGSSKTISLGTAKTLKIGAKTQTLSSDAA LPSDEDIRRMTGEGGS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t78c,N26N
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-2836c
Recommended expression in:
Not Applicable
Vector Name:
pSB2
Synonyms:
pSB-2, pSB2_TN
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds N-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
TOPO modified pSB2 vector -by Invitrogen. Stop codon generated by adding an extra G to the reverse primer which puts the insert in-frame with vector-encoded stop when cloned into the TA site.
Size (bp):
5338
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
TOPO recognition site
TOPO
TOPO cloning site
5110
5119
bacterial origin
ori
ColE1 bacterial origin of replication
1420
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4984
5003
repressor protein gene
LacI
LacI cds
3770
4594
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
6xHis tag
5079
5096
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 terminator
5267
5313
