BH3553 (H. halodurans )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BH3553
Gene Name:  ribonuclease R
Original Clone ID:
6231g1BCt29b1
Keyword:
full-length cds
Species:
Halalkalibacterium halodurans
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
BhCD00433010
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 2337nts
Open reading frame: 51 to 2387

CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCTTAATGAACAAA
AAGCGCAGCAACTGTTGATGTTCTTTCGTGAACAAGCTGAGAAGCCGCTATCTGTCAACG
AGCTAGAAGAAGCCTTAAACTTAGAAGACGCTCAAGAGTTTAAAGAGTTAGTACAGGTTT
TAAATGAAATGGAGGAAAAAGGTCTCCTCGTTCGAACGAGGAGCAATCGCTACGGTGTCC
CTGAAAAGATGAATTTACTTAGAGGAGTCGTCCAAGGGCATGCTAAAGGATTTGCGTTCG
TCATCCCAGAAGAAGAAGGACACTCCGATGTTTACGTCTCGCATGTGGATTTAGAAAGTG
CCATGAATGGGGACCGAGTGATCGTGCGCCTTCATCCTCATTCAACGGGAACCCGCCCTG
AGGGAAAGGTGATCCGCATTTTAGAGCGGGGCGTAAGCGAAGTGATCGGTACGTATGTTG
ACCACCAAACGTACGGCCTTGTCGTAGCCGACGACAAACGGATTCCGAACGACATTTTAA
TTGTAAACCCTGAAGCGCAAGGCCTTGTGGACGGTCACAAAGTCATTGTGAAGATTACGA
AATATCCAGAGGGGCGCATGAGTGCCGAAGGGGAAGTTATCAGCATTCTCGGTCATAAAA
ATGACCCTGGCATGGATATTTTATCGATCATTTATAAATATGGCATCCCGCAAGCATTCC
CGGAAGAGGTGCTACAACAAGCGCAAAACATCCCTGACGAAATTGATGCAGGCGAGATTA
AAAACCGCCGCGATTTACGTGACGAGCCGCTTGTGACGATCGATGGTGCTGATGCGAAAG
ATTTGGATGATGCTGTTCATGTGAGCCGGTTACCGAATGGCAATTTTAAGCTAGGTGTTC
ACATTGCCGATGTGAGCTATTACGTGAAAGAAAAGTCTCCGATCGATCTAGAAGCGGCTG
AGCGAGGGACGAGTGTCTATCTTGTGGACCGTGTCATTCCGATGATTCCGCACCGATTGT
CTAACGGGATTTGTAGTTTAAATCCTCAGGTCGATCGTCTCACCTTGTCATGCGAGATGG
AAATCACCCGTGATGGGCAAGTCGTCAATCACGAAATTTTTCAAAGCGTGATTCGCACAA
ATGAACGAATGACGTACCATGATGTAAACAAGATTCTCGTTGAAAAAGAGGAGGAGCTTC
GCAACCGCTACGAGCTGCTCGTCCCTATGTTTGAGGACATGGAGGAGCTTGCCGCGATCT
TACGTAAAAAGCGGTTCGGCCGTGGGGCGATCGATTTTGACTTTAAAGAAGCAAAAGTGC
TAGTGGATGAAGAAGGAAAACCATCAGATGTCGTCATTAGAGAGCGCGGTGTAGCGGAAA
AGCTCATTGAAGAGTTCATGCTTGCAGCAAACGAGACGGTTGCTGAACATTTCCATTGGC
TCAAGCTGCCGTTTATGTATCGAATTCATGAAGATCCAGACAGCGAAAAGCTAGGGCGCT
TCCTTGAGTTCATTACGAATTTCGGATATGTTGTCAGAGGAACGGCAAATACGGTTCACC
CTCGGGCACTGCAAAAGCTGTTAGAAGAAGTTCGCGGCGAACCAGAGGAGCAAGTCATTA
GCACAGTCATGCTCCGCTCAATGCAGCAAGCAAAATACGATCCGACAAGCCTTGGCCATT
TTGGACTTTCAACCGAGTTTTATACTCACTTTACGTCGCCTATTCGCCGTTACCCTGACT
TGATCGTCCATCGCCTCATCCGAGAATATTTAATCAAAGGGAATGTGGACGAAGAAACGC
AAGAGAAATGGCGTGAAAGGCTTCCTGAGATTGCCCGTCATTGTTCAGAAATGGAACGAC
GAGCCGTCGATGCTGAGCGAGAAACAGACGATGTGAAAAAGGCGCAATTCATGGAAGATA
AAATCGGTGAAACCTTTACGGGTATTATTAGCGGAGTGACGAATTTCGGAATTTTCGTGG
AGCTGGAAAATACGATTGAAGGGCTCGTTCACGTAAGCTATTTGACCGATGATTATTACC
GTTACGATGAAAAGCATTATGCGATGATCGGTGAACGTTCCGGCAATGTGTTTCGGATCG
GGGATGAAATCGAGGTCCGTGTCATTAATGTCAACGTTGAGGAAGCCTCGATTGATTTTG
AAATCGTCGGCATGAAGCCGCGCAAACAGCGTCCTGACAAAAGTCGTCCAAAAGTCATCG
AAGGCGGGAAGCGTAAGAAAAAAGAGAACCGAGGAGCCTTGGCTCTCGATGGCAGCAAGC
CGAAAAAGAAAAAGAAGAAAAAAGCGTTTTATGAAAATGCTCCGAGCGCCAAGCGGAAAA
AAGGGAAAAAGCGCAAGCGCGAAGGGCATCATCACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

NEQKAQQLLMFFREQAEKPLSVNELEEALNLEDAQEFKELVQVLNEMEEKGLLVRTRSNR
YGVPEKMNLLRGVVQGHAKGFAFVIPEEEGHSDVYVSHVDLESAMNGDRVIVRLHPHSTG
TRPEGKVIRILERGVSEVIGTYVDHQTYGLVVADDKRIPNDILIVNPEAQGLVDGHKVIV
KITKYPEGRMSAEGEVISILGHKNDPGMDILSIIYKYGIPQAFPEEVLQQAQNIPDEIDA
GEIKNRRDLRDEPLVTIDGADAKDLDDAVHVSRLPNGNFKLGVHIADVSYYVKEKSPIDL
EAAERGTSVYLVDRVIPMIPHRLSNGICSLNPQVDRLTLSCEMEITRDGQVVNHEIFQSV
IRTNERMTYHDVNKILVEKEEELRNRYELLVPMFEDMEELAAILRKKRFGRGAIDFDFKE
AKVLVDEEGKPSDVVIRERGVAEKLIEEFMLAANETVAEHFHWLKLPFMYRIHEDPDSEK
LGRFLEFITNFGYVVRGTANTVHPRALQKLLEEVRGEPEEQVISTVMLRSMQQAKYDPTS
LGHFGLSTEFYTHFTSPIRRYPDLIVHRLIREYLIKGNVDEETQEKWRERLPEIARHCSE
MERRAVDAERETDDVKKAQFMEDKIGETFTGIISGVTNFGIFVELENTIEGLVHVSYLTD
DYYRYDEKHYAMIGERSGNVFRIGDEIEVRVINVNVEEASIDFEIVGMKPRKQRPDKSRP
KVIEGGKRKKKENRGALALDGSKPKKKKKKKAFYENAPSAKRKKGKKRKREGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in NYSGXRC-6231g
Recommended expression in:
Not Applicable