Gene:
Gene Symbol:
fruA
Gene Name:
exo-beta-D-fructosidase
Original Clone ID:
6236c2BCt1b1
Keyword:
full-length cds
Species:
Streptococcus mutans
Type:
cDNA
Vector Name:
pSGX3
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
SmCD00433011
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 4182nts
Open reading frame: 51 to 4232
CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCTTGCTGAAGAAA
TAAGTCAAACAACTGCTGCTGATACTGCTGTTACAGAAGTAAGAACAGAAGACAGCAGCC
AGACGTCATCTCAAGAAACCGCAGTTACAGAAACGACTCAATCTGAGGGAACTGCTTCAA
AACAACTGACAACTCCGGCGGTAGCAGATCAAACTACAGAGCCTACTGATAATGAACCCA
TCAGTTCCTCTGATGGTGCGAGCAGTCCTTATCAAGTGACTGACACGACAGAACCTCAAC
AAACTCTCACTCCTGCTGACTCTGAACCTCAAGCAAAGGCGGATGTTCAACAAGCAGCAG
CTCCTAAGAAAGAAGAGATCAACCCTGTGACCAATTTAGAAGATATGTCACATGATACGA
ATGGGACTTGGGAAGTACGAGAAGATGGTATTCATTCTAATGCCATTGGTAAAGGTGATT
CTTTCTTGTATTCTCAGTCAAGTGGTAAGAATTTTGTCTATGCAACAGATGTTACTTTTA
AACAAAACAGCGGTGCAGCAGCTCTTGTTTTTAGAAGTAACAACGATAGCAATAACAAAA
ATATGTATGCGGTCAATGTGGATATAGGCGGACACAAGGCTAAATTTTGGCGTTGGGTTG
ACAATAAAGATATCCAATTAATTGATGAACGTGATGTGGTACCGACAGCTGATAACCGCT
ATACTCTTAAGGTTGTGGCGGTTAATAACTGGATTTCTTATTATGTCAATGATATTTTGA
TGGCTAGCACAGGTGATTATGTCCTTCAAAAAGCTGATAAAGGACAAAACACTGTCATTC
CTGAGGGACACTTTGGTCTTTTGAATTGGAATGGCGATATGGTTTTCCAAAATACAAAAT
TTGCCCTATTGGATGATACAACAGCTCCGTTGATTGATAATATTACAGTAAGGTCTGATA
GAGGGAATGTCGAAAAACAAGGTCAATTCTTCTCTGAGGAACCCCTTCATATTCAATATG
TTAGCAATGATGCCTCTCAAGTGAGCTTGGATATTGCTAAACATAATCCTGCAGCTACTG
TCACAGTAGAAGATAAAACTGGTCGTGTTTACACAGATCCTTCTCATCTTCCAGTTAATG
TAGGAGCTAATTATTTCACTGTTAAAAGTACAGTTATTGATTCATTTGGTCGTACAGTAA
CCTTGACTTATCGTATTAATGTCCACCGTCGGCAAAATGACGAAGTCTATTATAATGAAC
TTTACCGCGACCAATATCACTATTCTGTAAAAGACGGCTGGGCTAATGATCCTAATGGCT
TGGTTTATTACAATGGTGTTTATCATCTCTTCCATCAATTTTACGATGATACGAAATGGG
GCCCTATGCACTGGGCGCACGCGACTAGTACGGATTTGATTCACTGGAAAGAAGAGCCAA
TTGCTTTTTACCCAGATTCAAACGGCTACATGTTCTCGGGCTGTGTAGTGGTTGACGAAC
ATAACAGTTCAGGCTTATTTAAGACGGCTAAGGGTGGTTTAGTTGCGATTATCACAGCTA
ATGGAAATGGACAGCGCATGGAACTTGCCTATAGTGAAGATGAAGGCAAGACTTGGCAGA
AATATGACAGGATTGTAGCCGATTGGTCAAATGACCCATTGCAAAATCAGGATTTTCGTG
ATCCAAAAGTTTTTCACTGGAATAACCAATGGTTCATGGTACTAGCAGGGGGTCCGCTTC
GCATTTATTCTTCTAACAATTTGAAAGATTGGAAAGTGGAGTCTACTTATCCTGATCTTC
ATACAGAATGTCCAGATATGTATCCAATTGTTGCTAATGATGGTGTGCTTAAATGGGTGT
TGTCTCGCGGCGGTCGTTTTTACAAGGTTGGTGATTTCAAACAAGTAGATGGTAAATGGA
CCTTTATAGCAGATGATGCTTATAAAGATAAGGATCAGGTCATGAACTTCGGTAAAGATT
CTTATGCCGCCATGACCTACTATGTACACGATTTTGGTACAGAAACCCGACCAACCATTC
CCAAATTAACAGAAGTCAACTGGATGAACACCTGGGAAGATTATTGTAATCTTGTAGCAG
ATACAGTTGGACAGGACTTTAACGGTACGTTTAATTTAAACCTTGATCTTGGCCTTATCA
ATGAAAACGGTCAGTACATCTTGACGCAAACGCCTGTTAAGGCCTATGATAGTCTTCGTG
ATGTGAATACTGCCTTGCATTTCAAAGATGTGACAGTAGACGCTAATAATACTCTTTTGA
AGGACTTCAAAGGAGACAGTTATGAAATTGTTTCTCATTTCCGACCAGATGAGAAGACCA
CAAAAGTAGGCTTCAATCTTCGTGTTGGCAATGGTCAAGCAACGAAAGTGATTTACGACT
TGCAGACAGAAACCTTGTCTATTGACCGTAGTCAGTCGGGTACTATCTTATCTGCAGCTT
TTGCAAAAGTTAATAGTCAGCATGTGACTAAAAATGCAGATGGCTCCATTGATTTGCATA
TTTATGTTGATCGTGCCAGTGTTGAAGTCTTTTCCAAAAATAATACAGTGGCTGGTGCTA
ATCAAATTTTTCCTAATCCAGAAGCTGTAGGTGCCAGTATTATTGTAGAAGGCGGCAAAG
CTCAAGCAGATATCTCTGTTTATCAAATGAAAACGATTTGGACAGATAAGAAAGATACGG
CAAAACCGGTGGCTATGAACACAACAACTGCGAAAGAGCTAGCCCTTCAAGTTGGTCAAA
GTCAGGATCTGCAAGTCTATCTGGCACCAGCAAGTGTTAGGCAAGATGTGGAATGGACGA
TCAGTGATCCAAGTCTTGTTAGGACAAGTCAAAAAGGTAATGTTCTTCATTTGACCGCTG
TGAAAAAAGGAAAGCTTACCATTACAGCAATTTCTAAAGAAAACCCAAGTCTCAGTAAAA
CCTTTACAATCAGTATCACCTTAAATAATTTCAAGACTAACCTCAAAGGTTTGCAGTCTG
TTACTGGTAAGTGGTATGTTGATGATGAAACGCTCTATGATAGTAATACAAGTTCGAATG
ATTACTATATGGCTTCTCAAAAACCGGGTTTCAAAGAATATGATTACGATATTGATCTCA
AATATCAACGTGGTTTAATTAATCTTTTTGTTGCTTCTGGCAATATTGATCCGAGTCAAG
CATATTCTGTACAATTTGGTGACAGTGAGACTGTTCGGCTTTACCGTTTTGCTGGTGATA
CTATTGCAGAAGCTAATATGGGTAAACGAATCAATGATGATCAATACCACCATATTAAAG
TCACAAAAACAAAAAATAGTATAATCATATCAGTAGATGGTCAAGAAGTGATGAGTCATA
ACTTTGATCAGGTGGACTCATACTTTAATGATGCTTATGTAGGTCTCGGTTTGTGGGACG
GAGCTGTTGAATTCCAAAACTTCTTTGTAACAGATCATGCGACTACTCCCAAACCAGATT
CTGATCCGACACCGCAACCAGATGCACCAGAAGCATTGGCTCAAGAAAGGGAATTGATTG
ACCCTGCAACTGGCGTCCGTGTCATTCTTCAAAAAGGGGAGCTAGCTTCTATCGTTAGAG
TAAAGGTCAGCCATATCGAAACGAATGATGCTCATACACCAGCTGTTCTAAATGCAAAAG
ACTATGATCTCTTCAATATCACACCTATTGATAAAAATGAGAAAGTTGTTGCTATTACGA
AACCAGCGACAGTCTTATTGCCAATTGATGCTGGAAAAGTGGTAGATAAAGTGGTGTATT
TACCAAATACGGACAAAGAAGAAAACCTTCCATTTACGATTGTGAGTTTGACAGATAGCA
ATGGTAAGAAGCAAAGTTATGTTCGCTTTACAGCAGAACACTTTAGTGAATACGGCTTAG
TCTATCAAGCAGAAAATCAAACAAACCTTAAGAGTAAAGAAAAGCAAGACAATGTTGCTA
TTTCTTATCCATTAAATTTAGAACAAGAAGTTAAGGTTTCATCTATTTCACGAAAATATG
CTGCAAATAAGACAGCAGATGTCAATAGTGTTCAACAAACAGAACCTTCAGTAATGAGTT
CTTCACCTAAAGCAACGCTGCCAGATACTGGTGATCATAAGACAGATTTAAGTCAATTAG
GAGTGTTGGCAATGATTGGCTCATTTCTTGTAGAAATAGCTGGCTATTTCAAAAAACGCA
AGGATGAAGGGCATCATCACCATCACCACTGATCCGAATTCGAGCTCCGTCGACAAGCTT
GCGGCCGCACTCGAGCACCACCA
Protein sequence predicted based on nucleotide sequence
AEEISQTTAADTAVTEVRTEDSSQTSSQETAVTETTQSEGTASKQLTTPAVADQTTEPTD NEPISSSDGASSPYQVTDTTEPQQTLTPADSEPQAKADVQQAAAPKKEEINPVTNLEDMS HDTNGTWEVREDGIHSNAIGKGDSFLYSQSSGKNFVYATDVTFKQNSGAAALVFRSNNDS NNKNMYAVNVDIGGHKAKFWRWVDNKDIQLIDERDVVPTADNRYTLKVVAVNNWISYYVN DILMASTGDYVLQKADKGQNTVIPEGHFGLLNWNGDMVFQNTKFALLDDTTAPLIDNITV RSDRGNVEKQGQFFSEEPLHIQYVSNDASQVSLDIAKHNPAATVTVEDKTGRVYTDPSHL PVNVGANYFTVKSTVIDSFGRTVTLTYRINVHRRQNDEVYYNELYRDQYHYSVKDGWAND PNGLVYYNGVYHLFHQFYDDTKWGPMHWAHATSTDLIHWKEEPIAFYPDSNGYMFSGCVV VDEHNSSGLFKTAKGGLVAIITANGNGQRMELAYSEDEGKTWQKYDRIVADWSNDPLQNQ DFRDPKVFHWNNQWFMVLAGGPLRIYSSNNLKDWKVESTYPDLHTECPDMYPIVANDGVL KWVLSRGGRFYKVGDFKQVDGKWTFIADDAYKDKDQVMNFGKDSYAAMTYYVHDFGTETR PTIPKLTEVNWMNTWEDYCNLVADTVGQDFNGTFNLNLDLGLINENGQYILTQTPVKAYD SLRDVNTALHFKDVTVDANNTLLKDFKGDSYEIVSHFRPDEKTTKVGFNLRVGNGQATKV IYDLQTETLSIDRSQSGTILSAAFAKVNSQHVTKNADGSIDLHIYVDRASVEVFSKNNTV AGANQIFPNPEAVGASIIVEGGKAQADISVYQMKTIWTDKKDTAKPVAMNTTTAKELALQ VGQSQDLQVYLAPASVRQDVEWTISDPSLVRTSQKGNVLHLTAVKKGKLTITAISKENPS LSKTFTISITLNNFKTNLKGLQSVTGKWYVDDETLYDSNTSSNDYYMASQKPGFKEYDYD IDLKYQRGLINLFVASGNIDPSQAYSVQFGDSETVRLYRFAGDTIAEANMGKRINDDQYH HIKVTKTKNSIIISVDGQEVMSHNFDQVDSYFNDAYVGLGLWDGAVEFQNFFVTDHATTP KPDSDPTPQPDAPEALAQERELIDPATGVRVILQKGELASIVRVKVSHIETNDAHTPAVL NAKDYDLFNITPIDKNEKVVAITKPATVLLPIDAGKVVDKVVYLPNTDKEENLPFTIVSL TDSNGKKQSYVRFTAEHFSEYGLVYQAENQTNLKSKEKQDNVAISYPLNLEQEVKVSSIS RKYAANKTADVNSVQQTEPSVMSSSPKATLPDTGDHKTDLSQLGVLAMIGSFLVEIAGYF KKRKDEGHHHHHH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-6236c
Recommended expression in:
Not Applicable
Vector Name:
pSGX3
Synonyms:
pSGX-3, pSGX3_BC
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
Modified from pET26b from Novagen
Size (bp):
5339
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
initiation start (Met, Ser, Leu)
5072
5079
TOPO recognition site
TOPO
TOPO cloning site
5076
5085
bacterial origin
ori
pBR322-type bacterial origin of replication
1421
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4983
5003
repressor protein gene
LacI
LacI CDS
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
6xHis tag
5086
5103
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 terminator
5231
5307
