44483596 (E. genome shotgun sequencing of the Sargasso Sea )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
9372c1BCt10p1
Keyword:
full-length cds
Species:
Environmental genome shotgun sequencing of the Sargasso Sea
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
EgCD00433048
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1197nts
Open reading frame: 1 to 1197

ACCAAAATTAAGAATATCCAAACCCAAGTGTATCCGTGGAACCGCCAGACCCTTCCCCCC
AAAAACAACTTTTGCACCAACCCCAGCGATATACTGTACCAAAAATCTGACCCCCTGGGT
AGTTTCCGTTTTCCTGAGTGGTTAATCTGTGAGATCGAGACTGACGATGGGGTATTCGGA
ATTGGCAATGCAGCTTTGGCGCCTCAAATTGTAAAGAAAACAATAGATACTTATCTGAAA
CCGCTTGTGGTTGGTGAGGACCCCTTTGATTACGCCTATATTTGGGAAAAGATGTACCGC
CGGACACACGCATGGGGGAGACGAGGAATCGGCATGATTGCTATGTCAGCGATAGATATC
GGTATTTGGGACATCCTCGGCAAAATTACCAATAAACCAGTATTCAAGCTGTTAGGTGGG
CGTACGAAAGAAAAAATACCTGTATATGCCTCCAAGTTGTACTGGCAGCCGCTTAATGAA
CTGCAAAAAGAGGCAGAAACCTATAAAAATCAGGGATTTAAGATGTATAAAATGAGCTTC
GGCTGGGGTCCCAAAGATGGCCCGGAAGGTATGAAGAAAAACATCGACCTCGTGGAGGCT
GTTCGCGAAGTCATTGGGGATGACTCTGATCTGATGTTAGAGAGTTACATGGGATGGAAT
CTGGATTATTCAAAACGTATAATCCCAAAGTTGATTAAATTTAACCCTCGCTGGCTTGAA
GAACCGGTGATTGCGGACGATATCGCCGGCTACTCCGAGCTGAATAAAATGGGTGTAATT
CCCATATCGGGGGGCGAACATGAGTTCAACCTCTTTGGTTTTAAGCAGTTACTGGACTTG
AAAGCAGTTAGCTATATCCAATACGATACTAATCGGGTGGGAGGCATTACAGCTGCGCAG
AAAATCAACGCCCTTGCAGAATCTTTCCAAGTCCCGGTTGTACCACACGCGGGTCAGATG
CATAATTATCACCTGACGATGGCTAACTTTAATTGCCCTATTAGTGAATACTTCCCGGTG
CATGATGTCGAGGTTGGGAACGAATTATTTTATTATATATTCGAAGGCGACCCAGAGCCG
AAGAATGGTAACATCAATCTGGATGACAACATTCCCGGACTCGGCCTGAAAATATCAGAT
AAATACAAGAGCGACTTTAATATCATTGATGAAGGGCATCATCACCATCACCACTGATCC
GAATTCGAGCTCCGTCGACAA

Protein sequence predicted based on nucleotide sequence

TKIKNIQTQVYPWNRQTLPPKNNFCTNPSDILYQKSDPLGSFRFPEWLICEIETDDGVFG
IGNAALAPQIVKKTIDTYLKPLVVGEDPFDYAYIWEKMYRRTHAWGRRGIGMIAMSAIDI
GIWDILGKITNKPVFKLLGGRTKEKIPVYASKLYWQPLNELQKEAETYKNQGFKMYKMSF
GWGPKDGPEGMKKNIDLVEAVREVIGDDSDLMLESYMGWNLDYSKRIIPKLIKFNPRWLE
EPVIADDIAGYSELNKMGVIPISGGEHEFNLFGFKQLLDLKAVSYIQYDTNRVGGITAAQ
KINALAESFQVPVVPHAGQMHNYHLTMANFNCPISEYFPVHDVEVGNELFYYIFEGDPEP
KNGNINLDDNIPGLGLKISDKYKSDFNIIDEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a4c,K2Q;a19c,K7Q;
a25c,K9Q;a35c,Q12P;
g43c,G15R;a46c,K16Q;
g52c,V18L;g57c,P19P;
a60c,P20P;g78c,T26T;
g82c,g84c,A28P;g100c,E34Q;
g112c,A38P;a115c,M39L;
a134c,H45P;c174a,V58V;
a258g,E86E;c402a,V134V;
c464g,S155W
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Tested_Not_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-9372c
Recommended expression in:
Not Applicable