thiH (S. enterica subsp. enterica serovar Typhimurium str. LT2 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  thiH
Gene Name:  thiamine biosynthesis protein ThiH
Original Clone ID:
11100i1BCt18p1
Keyword:
full-length cds 2-377 of 377 aa
Species:
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
StCD00433160
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1155nts
Open reading frame: 51 to 1205

CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCTTAAAACCTTCA
CCGACCGTTGGCGGCAACTGGAGTGGGACGATATTCGCCTGCGCATCAATGGTAAAACCG
CCGCTGACGTGGAACGTGCGCTGAACGCCGCGCATCTTAGCCGGGATGATTTGATGGCAC
TGCTCTCCCCCGCCGCCGCCGATTATCTGGAACCGATAGCGCAGCGGGCGCAAAGGCTGA
CCCGACAACGCTTTGGCAACACCGTCAGTTTCTACGTGCCGCTTTATCTCTCAAACCTCT
GTGCCAACGACTGTACCTACTGCGGTTTTTCGATGAGCAACCGCATTAAGCGTAAAACGC
TGGATGAGGTGGATATTCAAAGGGAATGCGATGCTATCCGTAAACTGGGCTTTGAGCATC
TGCTGTTAGTCACCGGCGAACATCAGGCCAAAGTCGGCATGGACTATTTTCGCCGTCATT
TACCCACCATCCGCCGTCAATTTTCCTCTTTACAGATGGAAGTCCAGCCCTTGTCGCAAG
AAAACTATGCGGAGCTCAAAACACTGGGGATCGATGGCGTGATGGTTTATCAGGAGACTT
ATCATGAGGCAATCTATGCACAGCATCACCTGAAGGGAAAGAAACAGGACTTTTTCTGGC
GGCTGGAAACGCCGGATCGGTTAGGCCGGGCAGGTATCGACAAAATCGGTCTTGGCGCGC
TAATTGGTCTGTCGGACAACTGGCGGGTGGATTGCTATATGGTGGCGGAGCATCTGTTGT
GGATGCAGAAACACTACTGGCAGAGTCGCTATTCTGTTTCCTTCCCGCGTCTGCGTCCGT
GTACTGGCGGTGTGGAACCCGCATCTGTGATGGATGAAAAGCAACTGGTGCAAACGATTT
GCGCTTTCCGGTTATTGGCGCCGGAAATTGAATTATCACTCTCCACCCGCGAATCGCCGT
GGTTTCGAGACCATGTGATTCCTCTGGCAATCAACAACGTCAGCGCCTTCTCAAAAACGC
AGCCCGGCGGCTACGCTGACGATCATCCGGAACTGGAGCAGTTTTCTCCCCACGATGCCC
GTCGGCCTGAAACAGTAGCAAGCGCGTTAAGCGCGCAAGGATTGCAGCCCGTCTGGAAAG
ACTGGGACAGTTGGCTGGGGCGCGCTTCGCAAACGCGGGAAGGGCATCATCACCATCACC
ACTGATCCGAATTCGAGCTCCGTCGA

Protein sequence predicted based on nucleotide sequence

KTFTDRWRQLEWDDIRLRINGKTAADVERALNAAHLSRDDLMALLSPAAADYLEPIAQRA
QRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKRKTLDEVDIQRECDAIRKLG
FEHLLLVTGEHQAKVGMDYFRRHLPTIRRQFSSLQMEVQPLSQENYAELKTLGIDGVMVY
QETYHEAIYAQHHLKGKKQDFFWRLETPDRLGRAGIDKIGLGALIGLSDNWRVDCYMVAE
HLLWMQKHYWQSRYSVSFPRLRPCTGGVEPASVMDEKQLVQTICAFRLLAPEIELSLSTR
ESPWFRDHVIPLAINNVSAFSKTQPGGYADDHPELEQFSPHDARRPETVASALSAQGLQP
VWKDWDSWLGRASQTREGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-11100i
Recommended expression in:
Not Applicable