Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
N-His_Rv2614c
Keyword:
None
Species:
Mycobacterium tuberculosis H37Rv
Type:
cDNA
Vector Name:
pET28-N-His-thromb
Format:
FUSION
Source:
Integrated Center for Structure and Function Innovation
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Integrated Center for Structure and Function Innovation
Integrated Center for Structure and Function Innovation
MtCD00433214
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 2076nts
Open reading frame: 44 to 2119
TCATCATCATCACAGCAGCGGCCTGGTGCCGCGCGGCAGCCATATGAGCGCCCCCGCACA
ACCCGCCCCGGGAGTCGATGGCGGCGACCCGTCGCAAGCCCGAATTCGGGTTCCTGCCGG
GACCACCGCGGCCACCGCCGTCGGCGAAGCGGGTTTACCGCGGCGCGGTACGCCCGATGC
GATCGTCGTCGTGCGCGACGCCGACGGCAACCTGCGCGACCTGAGCTGGGTGCCCGACGT
CGACACCGATATCACGCCGGTGGCCGCCAACACCGACGACGGTCGCAGCGTGATCCGCCA
TTCGACCGCGCACGTGTTGGCCCAAGCCGTCCAAGAGCTGTTTCCGCAGGCCAAGCTCGG
CATCGGACCACCCATCACCGACGGCTTCTACTACGACTTCGACGTGCCCGAGCCGTTCAC
GCCCGAGGACTTGGCGGCGCTGGAAAAGCGGATGCGCCAGATCGTCAAGGAAGGCCAGCT
GTTCGACCGGCGGGTCTACGAATCCACCGAACAGGCCCGCGCCGAGCTGGCCAACGAGCC
CTACAAGCTGGAACTCGTCGACGACAAATCGGGTGACGCCGAGATCATGGAGGTCGGCGG
TGACGAGCTCACCGCCTACGACAACCTCAACCCCCGCACCCGCGAGCGCGTCTGGGGCGA
CCTGTGCCGCGGACCGCACATCCCGACCACCAAACACATCCCGGCGTTCAAGCTCACCCG
CAGCTCGGCCGCCTACTGGCGGGGCGATCAGAAAAACGCCAGCCTGCAACGGATCTACGG
CACCGCGTGGGAATCCCAGGAGGCGCTCGACAGGCACCTGGAGTTCATCGAAGAGGCGCA
GCGCCGCGACCACCGCAAGCTGGGTGTCGAGCTGGACCTGTTCAGCTTCCCCGACGAAAT
CGGTTCCGGCCTAGCGGTTTTCCACCCCAAGGGCGGCATCGTGCGTCGCGAACTGGAGGA
CTACTCGCGGCGCAAGCACACCGAGGCGGGCTACCAGTTCGTCAACAGCCCGCACATCAC
CAAGGCCCAGTTGTTCCACACCTCGGGACATCTGGACTGGTACGCCGACGGCATGTTCCC
CCCGATGCACATCGACGCGGAGTACAACGCCGACGGCTCGCTGCGCAAACCCGGCCAGGA
CTACTACCTCAAGCCGATGAACTGCCCGATGCACTGCCTGATCTTCCGCGCGCGCGGGCG
ATCCTATCGGGAACTGCCGTTGCGGCTCTTCGAGTTCGGCACGGTGTATCGCTACGAGAA
GTCCGGTGTGGTGCACGGGTTGACCCGGGTGCGTGGGCTGACCATGGACGACGCGCACAT
CTTCTGCACCCGCGACCAGATGCGCGACGAGCTGCGGTCGCTGCTGCGGTTTGTGCTCGA
CCTGCTCGCCGACTACGGCCTCACCGACTTCTACCTCGAACTGTCCACCAAGGACCCGGA
GAAGTTCGTCGGCGCCGAGGAGGTCTGGGAGGAAGCCACCACCGTGCTGGCCGAGGTGGG
CGCCGAATCCGGGCTGGAGCTGGTGCCCGATCCAGGCGGCGCGGCGTTCTACGGGCCCAA
GATTTCAGTGCAGGTCAAAGACGCGCTGGGCCGCACCTGGCAGATGTCGACCATCCAGCT
GGACTTCAACTTTCCGGAACGTTTCGGCCTGGAGTACACCGCCGCCGACGGAACCCGCCA
CCGCCCGGTGATGATCCACCGCGCGCTATTTGGGTCGATCGAGCGGTTCTTCGGCATTCT
CACCGAGCACTACGCGGGGGCGTTCCCGGCCTGGTTGGCGCCCGTGCAGGTGGTCGGCAT
CCCGGTCGCCGATGAGCACGTCGCCTATCTGGAAGAGGTTGCCACGCAACTGAAGTCGCA
CGGGGTGCGGGCCGAGGTGGACGCCAGCGACGATCGGATGGCCAAGAAGATCGTGCACCA
CACCAACCACAAGGTGCCGTTCATGGTGTTGGCGGGTGATCGTGACGTCGCCGCCGGCGC
GGTGAGTTTCCGGTTCGGTGACCGCACCCAAATCAACGGTGTGGCCCGTGACGATGCGGT
GGCGGCCATTGTCGCCTGGATCGCTGACCGCGAAAATGCGGTTCCTACAGCGGAACTGGT
GAAAGTGGCCGGTCGTGAGGGATCCTAGGGCTCCGCTGCTGGTTCTGGCGAATTCAGCAA
AGGAGAAGAA
Protein sequence predicted based on nucleotide sequence
MSAPAQPAPGVDGGDPSQARIRVPAGTTAATAVGEAGLPRRGTPDAIVVVRDADGNLRDL SWVPDVDTDITPVAANTDDGRSVIRHSTAHVLAQAVQELFPQAKLGIGPPITDGFYYDFD VPEPFTPEDLAALEKRMRQIVKEGQLFDRRVYESTEQARAELANEPYKLELVDDKSGDAE IMEVGGDELTAYDNLNPRTRERVWGDLCRGPHIPTTKHIPAFKLTRSSAAYWRGDQKNAS LQRIYGTAWESQEALDRHLEFIEEAQRRDHRKLGVELDLFSFPDEIGSGLAVFHPKGGIV RRELEDYSRRKHTEAGYQFVNSPHITKAQLFHTSGHLDWYADGMFPPMHIDAEYNADGSL RKPGQDYYLKPMNCPMHCLIFRARGRSYRELPLRLFEFGTVYRYEKSGVVHGLTRVRGLT MDDAHIFCTRDQMRDELRSLLRFVLDLLADYGLTDFYLELSTKDPEKFVGAEEVWEEATT VLAEVGAESGLELVPDPGGAAFYGPKISVQVKDALGRTWQMSTIQLDFNFPERFGLEYTA ADGTRHRPVMIHRALFGSIERFFGILTEHYAGAFPAWLAPVQVVGIPVADEHVAYLEEVA TQLKSHGVRAEVDASDDRMAKKIVHHTNHKVPFMVLAGDRDVAAGAVSFRFGDRTQINGV ARDDAVAAIVAWIADRENAVPTAELVKVAGRE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
ProteinExpressed: Tested_Not_Found
Find experimental protocols in N-His_Rv2614c
Recommended expression in:
Not Applicable
Vector Name:
pET28-N-His-thromb
Synonyms:
N-His-Thrombin pET28
Sequencing Primer:
Forward:
T7
Reverse:
ISFI_newrev
Description:
Expression vector with T7 promoter, adds N-terminal His tag, thrombin site; kanamycin resistance; restriction enzyme cloning.
Comments:
None
Size (bp):
5369
Parent Vector:
None
Properties:
cell-free expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name:
Integrated Center for Structure and Function Innovation
Publications:
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
1421
2103
primer
reverse
reverse sequencing primer 5?-TTTGTTAGCAGCCGGATCTCAGTGG-3?
5861
5885
promoter
T7
T7 promoter
4987
5006
protease cleavage site
Thrombin
N-terminal His/thrombin cleavage site
0
0
repressor protein gene
LacI
LacI
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
N-terminal 6xHis tag
5847
5864
trxn regulatory element
lac operator
lac operator
5006
5028
