BT_2239 (B. thetaiotaomicron VPI-5482 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BT_2239
Gene Name:  carboxyl-terminal protease
Original Clone ID:
44772
Keyword:
SP or TM removed
Species:
Bacteroides thetaiotaomicron VPI-5482
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
BtCD00433325
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1632nts
Open reading frame: 51 to 1682

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCTGTAAAGATG
ATGACACTCTACTGCGAGGTAGTGGAATTACAGAACAAAGCTGGTCTACTAACCAAACAT
ATTTTGCCTCAGCAGAGCAAACCCTCACTTTTACTTTCACAACCTTGTCTAGCTGGACTG
CTCAAAACAGTTCCACTGCATTATTGTCATTAGACAATACCGCAGGAAATTCAGGGGAAA
ATACCATAAAAGTTACAGTACACAAGTCTTCCCAGGAACAGGGCACCATTACGATTAAAG
TAAATGGATATTCATCTGCCAGCAATATCAAAATCCAGTTGAGCGATGATGATGTGCAAG
GTTATGAGATAAACTATAGTGTGGATCAATATTTGAGAGAAAAATACTTATGGAATGATG
ATTATAAGTTATTAACGCCCAATTTCAGACAAGCTTATGATGAGTTTTTGAGAAACACAT
TATTAAGCATGACAACTAATACATTAGACAAAAAACGAAATAGTAATGGCACTTATTCAT
TGTTTTCATTTATCCAGAAGCTAGATCCGGATTTACAAACTTCCCGCAGTGCAAAAGAGA
AAAAAACACTAGAGTATAATTATGGATTCGTAAACTTTATAGCCGTTGGAAACCGTAATA
ATTCAAATTATGGTCTTGTTATACAAGGAGTGCATAAAGGATCATCTGCTGATAAAGAAG
GACTAAAACGAGGAATGGAAATAACCGAAATAGACAATCAGAGAATAACAACGACTAACG
TGCAAGCTTGTTACTCTAAATTAATTAAACCATCCTCTCCAACCTCTATAAAAGTAAAAG
ACAAAGATGGAAAAGTATATACAATAAATTCCGGACCTATTTATGCGAACCCGATAATTC
ACCACCAAGTAAACGAAAAGATCGGCTATCTGGTGTATTCAGCTTTTGAGTCCGGCTTCG
ACCAAGAACTATTCGATGTATTCAAAGAGTTTAAAAGTCAGAATATTACAGAGCTAATTT
TAGATCTTCGCTACAATGGCGGAGGAGATGTAACATCGGCTAATCTTATATCCAGCTGCA
TTGCCGGAGATTTATGCATAGATAAAACATTCGCTTCATACCGATATAACGATGAACGCA
TGAAGGTCCTTGGCAACCAACGGCCTATTCAGAAATTCGCATATTCTCAATATGATAATT
TGAGTACTTCCCTCTCTGCCGGTGGTTTAAATTTACGAAAAATCTACTGTCTTGTTACTG
ACGACTCTGCATCTGCAAGTGAATTAGTAATCAATGCACTGAGAGGTATTGATATCGAAG
TTATCCTTATTGGCACCACCACACATGGGAAAAATGTAGGAATGGAAGGAGTAGAACTTA
CTGCAGGAACAGACAAATATTTGCTTTTCCCTATCACTTTCCAAGCATACAATGCCAAAG
GTTTTGGAGACTTCGAGAACGGTTTTACACCCGATTACGAGATAAATGAGAACAAGCCTA
ACGGAGAATATTTTGAAGGATATGGAGACTTTGGAGCTGAAAGCGATCCTTTATATGCCA
AAGCAATCAGTCTGATTTCAGGAAATGAAGTTACTACTCCTACAAGGGCAGTTAACCAAG
CTAAAGAACAAATGCTAGTGATAGCTACACCAAGATTGAACCGTATAGGAATGATAAAAT
AACGCGACTTAATTAACTCGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGG

Protein sequence predicted based on nucleotide sequence

CKDDDTLLRGSGITEQSWSTNQTYFASAEQTLTFTFTTLSSWTAQNSSTALLSLDNTAGN
SGENTIKVTVHKSSQEQGTITIKVNGYSSASNIKIQLSDDDVQGYEINYSVDQYLREKYL
WNDDYKLLTPNFRQAYDEFLRNTLLSMTTNTLDKKRNSNGTYSLFSFIQKLDPDLQTSRS
AKEKKTLEYNYGFVNFIAVGNRNNSNYGLVIQGVHKGSSADKEGLKRGMEITEIDNQRIT
TTNVQACYSKLIKPSSPTSIKVKDKDGKVYTINSGPIYANPIIHHQVNEKIGYLVYSAFE
SGFDQELFDVFKEFKSQNITELILDLRYNGGGDVTSANLISSCIAGDLCIDKTFASYRYN
DERMKVLGNQRPIQKFAYSQYDNLSTSLSAGGLNLRKIYCLVTDDSASASELVINALRGI
DIEVILIGTTTHGKNVGMEGVELTAGTDKYLLFPITFQAYNAKGFGDFENGFTPDYEINE
NKPNGEYFEGYGDFGAESDPLYAKAISLISGNEVTTPTRAVNQAKEQMLVIATPRLNRIG
MIK*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 393044
Recommended expression in:
Not Applicable