NFAT5 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  NFAT5
Gene Name:  nuclear factor of activated T-cells 5, tonicity-responsive
Original Clone ID:
NFAT5_pDONR221_fusion
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
Center for Personalized Diagnostics
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
Brianne Petritis
HsCD00433452
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 4647nts
Open reading frame: 1 to 4647

ATGCCCTCGGACTTCATCTCATTGCTCAGCGCGGACCTAGACCTGGAATCGCCCAAGTCC
CTCTACTCGCGAGATTCTCTGAAGTTACACCCATCACAGAATTTTCATAGAGCTGGACTA
TTGGAAGAATCTGTCTATGATCTTCTCCCAAAGGAGTTACAGTTACCTCCATCTAGAGAA
ACATCTGTAGCATCAATGAGTCAGACAAGCGGTGGTGAGACAGGCTCGCCTCCTCCAGCT
GTTGTTGCTGCTGATGCTTCTTCAGCTCCCTCCTCTTCCTCCATGGGCGGTGCTTGCAGC
TCCTTTACCACCTCTTCCAGCCCTACCATTTATTCTACCTCAGTCACCGACAGCAAGGCT
ATGCAAGTGGAGAGCTGCTCCTCAGCCGTGGGGGTAAGTAACAGAGGGGTAAGTGAAAAG
CAGTTAACCAGTAACACAGTTCAGCAGCATCCATCAACACCGAAGAGGCACACAGTCTTG
TACATCTCACCACCACCTGAGGACTTGCTGGATAACAGTCGGATGTCCTGCCAGGATGAG
GGGTGTGGATTGGAATCTGAGCAGAGCTGCAGTATGTGGATGGAGGATTCCCCCTCCAAC
TTCAGTAACATGAGCACCAGTTCCTACAATGATAACACTGAGGTACCTCGTAAATCACGA
AAACGAAATCCAAAGCAGAGGCCGGGGGTCAAACGACGAGATTGTGAAGAATCTAATATG
GATATATTTGATGCCGACAGTGCCAAAGCACCTCACTATGTGCTTTCTCAGCTTACCACG
GACAACAAAGGCAACTCAAAAGCGGGAAATGGAACATTGGAAAACCAAAAAGGAACTGGA
GTAAAGAAGAGCCCTATGTTGTGTGGACAATATCCTGTTAAAAGTGAGGGAAAGGAGCTG
AAGATAGTTGTACAACCTGAGACACAGCACCGAGCTCGGTACCTGACTGAGGGCAGCCGT
GGCTCAGTGAAAGATAGAACACAGCAAGGCTTTCCTACAGTAAAGCTGGAAGGCCATAAT
GAACCTGTAGTGTTGCAAGTGTTTGTGGGCAACGACTCTGGACGAGTGAAACCACATGGA
TTTTATCAGGCCTGCAGAGTAACTGGACGAAATACAACTCCTTGCAAAGAAGTGGACATT
GAAGGCACTACTGTTATAGAAGTCGGCCTTGATCCTAGCAACAACATGACACTGGCGGTG
GACTGCGTAGGGATATTGAAATTGAGGAATGCTGATGTCGAAGCCAGAATAGGAATTGCT
GGTTCCAAGAAGAAAAGCACTCGTGCCAGATTGGTTTTTCGAGTTAATATCATGAGGAAA
GATGGCTCCACTTTGACACTGCAAACACCCTCTTCTCCAATTTTGTGTACTCAGCCAGCA
GGAGTGCCAGAAATCTTAAAGAAAAGCTTGCATAGCTGTTCAGTGAAAGGAGAAGAAGAA
GTGTTTTTAATCGGCAAGAACTTTCTGAAAGGAACTAAAGTTATTTTCCAAGAAAATGTT
TCTGATGAAAACTCTTGGAAGTCAGAAGCTGAAATTGATATGGAACTATTTCATCAGAAT
CATCTTATTGTGAAGGTTCCTCCCTATCATGACCAACATATAACTTTGCCTGTGTCAGTG
GGAATATATGTAGTGACAAATGCTGGAAGATCTCATGATGTTCAACCATTCACTTACACT
CCAGACCCAGCAGCTGGTGCTTTGAATGTAAATGTGAAGAAGGAAATATCTAGTCCAGCA
AGACCTTGCTCTTTTGAAGAGGCCATGAAAGCAATGAAAACTACTGGATGTAATTTAGAT
AAGGTAAATATTATCCCTAATGCCCTGATGACTCCACTCATACCAAGCAGTATGATTAAG
AGTGAAGATGTTACTCCAATGGAAGTAACAGCAGAAAAAAGATCTTCCACTATTTTTAAG
ACTACAAAGTCTGTTGGATCAACTCAGCAAACATTAGAAAACATCTCAAACATAGCAGGA
AATGGCTCTTTTTCATCACCATCATCTTCCCACCTACCTTCTGAAAATGAAAAACAGCAG
CAGATTCAGCCCAAGGCATACAACCCAGAGACCCTGACAACTATTCAAACCCAGGACATC
TCACAGCCTGGTACTTTTCCAGCAGTTTCTGCTTCTAGTCAGCTGCCCAACAGCGATGCA
CTATTGCAGCAGGCTACACAGTTTCAGACAAGAGAAACTCAGTCTAGAGAGATATTACAG
TCAGATGGTACAGTGGTTAATTTGTCACAACTGACTGAGGCATCACAACAACAGCAGCAG
TCACCACTACAAGAACAAGCACAGACTTTACAGCAGCAGATTTCATCAAATATTTTTCCA
TCACCAAATAGTGTGAGTCAGCTTCAGAATACTATTCAGCAGCTGCAAGCAGGGAGTTTC
ACAGGCAGTACTGCTAGTGGCAGCAGTGGAAGTGTTGACTTGGTCCAACAAGTTTTAGAG
GCACAGCAGCAGTTATCTTCAGTTTTATTTTCTGCTCCAGATGGTAATGAGAATGTTCAA
GAGCAGCTTAGTGCAGATATTTTTCAACAAGTCAGTCAAATTCAGAGTGGTGTAAGCCCT
GGAATGTTTTCCTCAACAGAGCCAACAGTCCATACCAGACCAGATAATTTATTACCTGGA
AGAGCTGAAAGTGTTCATCCACAGTCTGAAAACACGTTATCTAATCAACAGCAGCAGCAG
CAGCAGCAACAGCAAGTGATGGAATCTTCAGCCGCAATGGTGATGGAGATGCAACAGAGT
ATCTGCCAGGCAGCTGCCCAGATTCAGTCAGAGTTATTCCCTTCAACTGCTTCAGCAAAT
GGAAACCTTCAGCAATCGCCAGTTTACCAGCAGACTTCTCACATGATGAGTGCATTGTCT
ACCAATGAGGATATGCAAATGCAGTGTGAATTGTTTTCTTCTCCTCCTGCAGTTTCTGGA
AATGAAACTTCTACAACTACCACACAGCAGGTTGCAACCCCTGGCACTACCATGTTTCAG
ACATCAAGTTCAGGAGATGGAGAAGAAACTGGAACACAAGCAAAACAGATTCAGAACAGT
GTCTTTCAGACCATGGTCCAAATGCAACATAGTGGGGACAATCAACCTCAAGTTAACCTT
TTTTCATCCACAAAAAGTATGATGAGTGTTCAGAATAGTGGTACCCAACAACAAGGTAAT
GGTTTATTCCAGCAAGGGAATGAGATGATGTCACTTCAATCTGGAAATTTTTTGCAGCAG
TCTTCTCATTCACAGGCCCAACTTTTTCATCCTCAAAATCCTATTGCCGATGCTCAGAAC
CTTTCCCAGGAAACTCAAGGTTCTCTCTTTCATAGTCCAAATCCTATTGTCCACAGTCAG
ACTTCTACAACCTCCTCTGAACAAATGCAGCCTCCAATGTTTCACTCTCAAAGTACCATT
GCTGTGTTACAGGGCTCTTCAGTTCCTCAAGACCAGCAGTCAACCAACATATTTCTTTCC
CAGAGTCCCATGAATAATCTTCAGACTAACACAGTAGCCCAAGAAGCATTTTTTGCAGCA
CCGAACTCAATTTCTCCACTTCAGTCAACATCAAACAGTGAACAACAAGCTGCTTTCCAA
CAGCAAGCTCCAATATCACACATCCAGACTCCTATGCTTTCCCAAGAACAGGCACAACCC
CCGCAGCAGGGTTTATTTCAGCCTCAGGTGGCCCTGGGCTCCCTTCCACCTAATCCAATG
CCTCAAAGCCAACAAGGAACCATGTTCCAGTCACAGCACTCAATAGTTGCCATGCAGAGT
AACTCTCCATCCCAGGAACAGCAGCAGCAGCAGCAACAGCAGCAGCAACAGCAGCAGCAA
CAACAACAGAGCATTTTATTCAGTAATCAGAATACCATGGCTACAATGGCGTCTCCAAAG
CAACCACCACCAAACATGATATTCAACCCAAATCAAAATCCAATGGCTAATCAGGAGCAA
CAGAACCAGTCAATTTTTCACCAACAAAGTAACATGGCCCCAATGAATCAAGAGCAACAG
CCCATGCAATTTCAGAGTCAGTCCACAGTTTCCTCACTTCAGAACCCAGGTCCTACCCAG
TCGGAATCATCACAGACCCCCTTGTTCCATAGCTCTCCTCAGATTCAGTTGGTACAAGGG
TCACCTAGTTCTCAAGAGCAGCAAGTAACTCTCTTCTTATCTCCAGCATCCATGTCTGCC
TTGCAGACCAGTATAAATCAACAAGATATGCAACAGTCTCCTCTTTATTCCCCTCAGAAC
AACATGCCTGGAATTCAAGGAGCCACATCTTCGCCTCAACCACAGGCTACTTTATTTCAC
AACACAGCAGGAGGCACAATGAACCAACTGCAGAATTCTCCTGGCTCATCTCAGCAGACA
TCAGGAATGTTCTTATTTGGCATTCAAAATAACTGTAGTCAGCTTTTAACCTCTGGACCA
GCTACATTGCCTGATCAGTTGATGGCCATAAGTCAGCCAGGCCAACCACAAAACGAGGGC
CAGCCACCTGTGACAACACTTCTTTCTCAGCAAATGCCAGAGAATTCTCCACTGGCATCC
TCTATAAACACCAACCAGAACATCGAAAAGATTGATTTGCTTGTTTCATTGCAAAACCAA
GGGAACAACTTGACTGGCTCCTTTTTG

Protein sequence predicted based on nucleotide sequence

MPSDFISLLSADLDLESPKSLYSRDSLKLHPSQNFHRAGLLEESVYDLLPKELQLPPSRE
TSVASMSQTSGGETGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKA
MQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDE
GCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNM
DIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKEL
KIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHG
FYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIA
GSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEE
VFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSV
GIYVVTNAGRSHDVQPFTYTPDPAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLD
KVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAG
NGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDA
LLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFP
SPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQ
EQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQ
QQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALS
TNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNS
VFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQ
SSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTI
AVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQ
QQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQS
NSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQ
QNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQG
SPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFH
NTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEG
QPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSFL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
A74T

g220a

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable