SLC4A4 (Homo sapiens) in pPICZa

Explanation of Terms

Gene:
Gene Symbol:  SLC4A4
Gene Name:  solute carrier family 4 (sodium bicarbonate cotransporter), member 4
Original Clone ID:
TransportPDB-0012
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pPICZa
Format: 
FUSION
Source:
Center for the X-ray Structure Determination of Human Transporters
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Geoffrey Chang
Tuan Nguyen
PSI:Biology
Center for the X-ray Structure Determination of Human Transporters
HsCD00433652
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 3234nts
Open reading frame: 1 to 3234

GAAGACGAGGCAGTCCTTGATAGAGGTGCTTCTTTCCTGAAGCACGTCTGTGACGAGGAA
GAGGTTGAGGGACACCATACTATCTACATTGGTGTTCATGTCCCAAAGTCTTATAGACGT
AGGCGACGCCACAAAAGAAAGACCGGTCATAAGGAAAAGAAAGAAAAAGAGAGAATCTCC
GAGAACTACTCCGACAAGTCAGATATTGAAAATGCTGACGAGTCTTCCTCAAGTATCTTG
AAGCCACTGATTTCTCCTGCTGCCGAGAGGATCCGATTCATTTTGGGTGAAGAGGACGAT
TCCCCAGCCCCACCTCAGCTGTTTACTGAACTTGACGAGTTGCTGGCTGTTGATGGTCAA
GAAATGGAGTGGAAGGAGACCGCCAGATGGATCAAGTTCGAAGAGAAAGTCGAACAGGGT
GGAGAGAGATGGTCTAAGCCACACGTTGCTACTCTCAGTTTGCATTCTCTTTTCGAATTA
CGTACCTGTATGGAGAAGGGTTCCATTATGCTGGACAGAGAAGCCAGCTCGTTGCCTCAG
CTGGTCGAGATGATCGTGGACCACCAAATTGAAACTGGTCTTTTAAAGCCAGAGTTGAAA
GATAAGGTCACTTACACCCTCCTAAGAAAGCACAGACATCAGACCAAGAAAAGTAACCTT
AGATCTTTAGCAGACATCGGAAAGACTGTTTCCTCAGCTTCTAGAATGTTCACCAACCCA
GATAATGGTTCCCCTGCTATGACACATAGAAACCTGACCAGTAGCTCTCTTAATGACATT
TCTGATAAGCCTGAGAAAGACCAATTGAAAAACAAGTTCATGAAGAAACTGCCAAGAGAT
GCTGAAGCCTCCAATGTGTTGGTAGGTGAGGTTGACTTTCTGGATACTCCATTCATCGCT
TTTGTCAGACTGCAGCAAGCCGTTATGTTGGGTGCACTGACTGAGGTCCCTGTGCCCACC
AGATTCTTGTTTATTTTGCTGGGTCCTAAAGGAAAGGCCAAATCTTACCACGAAATCGGT
AGAGCAATTGCTACCCTGATGTCCGACGAGGTTTTCCACGATATCGCCTATAAGGCAAAA
GACAGACATGATTTGATCGCTGGAATTGACGAATTTTTGGATGAGGTTATTGTCCTGCCC
CCGGGTGAATGGGACCCTGCCATCAGAATTGAGCCTCCCAAGTCTCTGCCATCCTCAGAT
AAGCGTAAAAACATGTACTCCGGCGGGGAGAACGTCCAGATGAATGGTGACACTCCACAC
GATGGAGGCCATGGTGGAGGCGGTCACGGAGACTGTGAAGAGTTGCAACGTACCGGTAGA
TTCTGCGGAGGCCTGATCAAGGATATTAAGAGAAAAGCTCCTTTCTTTGCCTCTGACTTT
TACGATGCTTTGAACATCCAGGCTTTGTCCGCCATCCTGTTCATTTATCTTGCTACAGTT
ACTAACGCCATTACTTTTGGTGGACTTTTAGGAGACGCTACCGATAATATGCAGGGTGTC
TTGGAGTCTTTCCTGGGTACTGCAGTTTCCGGAGCTATCTTCTGTTTGTTTGCCGGACAA
CCTCTTACTATTTTAAGTAGCACCGGTCCAGTGCTGGTTTTCGAAAGACTCCTATTCAAC
TTTTCTAAGGACAACAATTTCGATTACCTTGAGTTTAGATTGTGGATCGGTCTGTGGTCT
GCCTTCCTGTGCCTTATTTTAGTCGCAACCGACGCTTCCTTTTTGGTTCAATACTTCACC
AGATTTACAGAAGAGGGTTTCTCTTCCCTGATCTCCTTCATCTTTATTTATGACGCCTTT
AAGAAAATGATCAAGCTGGCAGATTACTATCCTATTAACTCTAATTTCAAGGTCGGTTAC
AACACTTTGTTTTCCTGTACCTGCGTTCCACCTGACCCAGCTAACATCTCCATTTCAAAT
GATACTACCTTGGCCCCAGAATACCTGCCTACTATGTCAAGTACCGACATGTATCACAAT
ACAACGTTCGATTGGGCTTTTTTGTCCAAGAAAGAGTGTTCAAAGTACGGCGGAAACCTG
GTCGGAAACAATTGCAATTTCGTTCCAGACATCACTTTGATGTCTTTCATTCTCTTTTTG
GGTACCTACACAAGCTCTATGGCTCTGAAGAAATTCAAGACCTCCCCCTATTTTCCTACC
ACAGCTAGAAAATTGATCTCTGACTTCGCCATCATTTTGTCCATCCTGATTTTTTGTGTC
ATCGACGCCTTGGTCGGTGTGGATACTCCAAAGCTGATTGTTCCTTCTGAGTTCAAACCA
ACCTCCCCTAACAGAGGATGGTTCGTCCCCCCATTTGGTGAGAATCCTTGGTGGGTTTGC
TTGGCAGCGGCCATTCCAGCTCTTTTAGTTACTATCCTGATTTTCATGGACCAGCAAATC
ACCGCCGTTATTGTCAACAGAAAGGAGCACAAACTGAAGAAAGGTGCCGGATACCATCTG
GATCTTTTCTGGGTCGCAATCTTGATGGTGATTTGTTCTCTTATGGCCTTACCATGGTAT
GTTGCAGCTACTGTCATCTCTATTGCTCACATCGACTCCCTGAAGATGGAAACTGAGACC
TCCGCCCCAGGAGAACAGCCTAAGTTCTTGGGTGTTAGAGAGCAACGTGTCACAGGAACT
CTGGTTTTTATTTTGACCGGTCTGTCTGTCTTCATGGCTCCAATCCTGAAGTTTATTCCA
ATGCCTGTGCTTTACGGTGTTTTCTTGTATATGGGAGTCGCCTCCTTGAACGGTGTTCAG
TTTATGGACAGATTGAAGCTCTTGCTGATGCCACTGAAACACCAACCTGATTTCATCTAC
CTGAGACACGTCCCACTTAGACGTGTTCATCTTTTCACTTTTTTGCAGGTTTTATGCCTC
GCTCTTTTATGGATCCTGAAGTCTACCGTCGCCGCAATAATTTTCCCTGTTATGATTCTT
GCCTTAGTGGCAGTTAGAAAGGGTATGGACTACTTGTTCAGTCAACACGATCTTTCTTTT
TTAGACGATGTCATCCCAGAGAAGGACAAGAAAAAGAAAGAAGATGAGAAGAAAAAGAAA
AAGAAAAAGGGTTCCTTGGACTCAGATAACGACGATTCCGACTGTCCTTATTCAGAAAAG
GTTCCATCCATCAAAATTCCTATGGATATTATGGAGCAGCAACCATTCTTGAGTGACAGC
AAGCCTTCTGATCGTGAAAGGTCCCCAACTTTTCTGGAGAGACACACCTCTTGC

Protein sequence predicted based on nucleotide sequence

EDEAVLDRGASFLKHVCDEEEVEGHHTIYIGVHVPKSYRRRRRHKRKTGHKEKKEKERIS
ENYSDKSDIENADESSSSILKPLISPAAERIRFILGEEDDSPAPPQLFTELDELLAVDGQ
EMEWKETARWIKFEEKVEQGGERWSKPHVATLSLHSLFELRTCMEKGSIMLDREASSLPQ
LVEMIVDHQIETGLLKPELKDKVTYTLLRKHRHQTKKSNLRSLADIGKTVSSASRMFTNP
DNGSPAMTHRNLTSSSLNDISDKPEKDQLKNKFMKKLPRDAEASNVLVGEVDFLDTPFIA
FVRLQQAVMLGALTEVPVPTRFLFILLGPKGKAKSYHEIGRAIATLMSDEVFHDIAYKAK
DRHDLIAGIDEFLDEVIVLPPGEWDPAIRIEPPKSLPSSDKRKNMYSGGENVQMNGDTPH
DGGHGGGGHGDCEELQRTGRFCGGLIKDIKRKAPFFASDFYDALNIQALSAILFIYLATV
TNAITFGGLLGDATDNMQGVLESFLGTAVSGAIFCLFAGQPLTILSSTGPVLVFERLLFN
FSKDNNFDYLEFRLWIGLWSAFLCLILVATDASFLVQYFTRFTEEGFSSLISFIFIYDAF
KKMIKLADYYPINSNFKVGYNTLFSCTCVPPDPANISISNDTTLAPEYLPTMSSTDMYHN
TTFDWAFLSKKECSKYGGNLVGNNCNFVPDITLMSFILFLGTYTSSMALKKFKTSPYFPT
TARKLISDFAIILSILIFCVIDALVGVDTPKLIVPSEFKPTSPNRGWFVPPFGENPWWVC
LAAAIPALLVTILIFMDQQITAVIVNRKEHKLKKGAGYHLDLFWVAILMVICSLMALPWY
VAATVISIAHIDSLKMETETSAPGEQPKFLGVREQRVTGTLVFILTGLSVFMAPILKFIP
MPVLYGVFLYMGVASLNGVQFMDRLKLLLMPLKHQPDFIYLRHVPLRRVHLFTFLQVLCL
ALLWILKSTVAAIIFPVMILALVAVRKGMDYLFSQHDLSFLDDVIPEKDKKKKEDEKKKK
KKKGSLDSDNDDSDCPYSEKVPSIKIPMDIMEQQPFLSDSKPSDRERSPTFLERHTSC

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a599n;a628n;a650n
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  Pichia    Marker:  zeocin
Host Type:  bacterial    Marker:  zeocin
Bacterial Selection Condition: 50 ug/ml zeocin
Growth Condition: Growth in low salt LB with 50ug/ml zeocin at 37 degrees C
Comments: None
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinPurified: Not_Tested
ProteinExpressed: Tested_Not_Found
Find experimental protocols in TransportPDB-0012
Recommended expression in:
Not Applicable