PCSK5 (Homo sapiens) in pLX304

Explanation of Terms

Gene:
Gene Symbol:  PCSK5
Gene Name:  proprotein convertase subtilisin/kexin type 5
Original Clone ID:
ccsbBroad304_06697
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00434335
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2740nts
Open reading frame: 1 to 2740

ATGGGCTGGGGGAGCCGCTGCTGCTGCCCGGGACGTTTGGACCTGCTGTGCGTGCTGGCG
CTGCTCGGGGGCTGCCTGCTCCCCGTGTGTCGGACGCGCGTCTACACCAACCACTGGGCA
GTCAAAATCGCCGGGGGCTTCCCGGAGGCCAACCGTATCGCCAGCAAGTACGGATTCATC
AACATAGGACAGATAGGGGCCCTGAAGGACTACTACCACTTCTACCATAGCAGGACGATT
AAAAGGTCAGTTATCTCGAGCAGAGGGACCCACAGTTTCATTTCAATGGAACCAAAGGTG
GAATGGATCCAACAGCAAGTGGTAAAAAAGCGGACAAAGAGGGATTATGACTTCAGTCGT
GCCCAGTCTACCTATTTCAATGATCCCAAGTGGCCCAGCATGTGGTATATGCACTGCAGT
GACAATACACATCCCTGCCAGTCTGACATGAATATCGAAGGAGCCTGGAAGAGAGGCTAC
ACGGGAAAGAACATTGTGGTCACTATCCTGGATGACGGAATTGAGAGAACCCATCCAGAT
CTGATGCAAAACTACGATGCTCTGGCAAGTTGCGACGTGAATGGGAATGACTTGGACCCA
ATGCCTCGTTATGATGCAAGCAACGAGAACAAGCATGGGACTCGCTGTGCTGGAGAAGTG
GCAGCCGCTGCAAACAATTCGCACTGCACAGTCGGAATTGCTTTCAACGCCAAGATCGGA
GGAGTGCGAATGCTGGACGGAGATGTCACGGACATGGTTGAAGCAAAATCAGTTAGCTTC
AACCCCCAGCACGTGCACATTTACAGCGCCAGCTGGGGCCCGGATGATGATGGCAAGACT
GTGGACGGACCAGCCCCCCTCACCCGGCAAGCCTTTGAAAACGGCGTTAGAATGGGGCGG
AGAGGCCTCGGCTCTGTGTTTGTTTGGGCATCTGGAAATGGTGGAAGGAGCAAAGACCAC
TGCTCCTGTGATGGCTACACCAACAGCATCTACACCATCTCCATCAGCAGCACTGCAGAA
AGCGGAAAGAAACCTTGGTACCTGGAAGAGTGTTCATCCACGCTGGCCACAACCTACAGC
AGCGGGGAGTCCTACGATAAGAAAATCATCACTACAGATCTGAGGCAGCGTTGCACGGAC
AACCACACTGGGACGTCAGCCTCAGCCCCCATGGCTGCAGGCATCATTGCGCTGGCCCTG
GAAGCCAATCCGTTTCTGACCTGGAGAGACGTACAGCATGTTATTGTCAGGACTTCCCGT
GCGGGACATTTGAACGCTAATGACTGGAAAACCAATGCTGCTGGTTTTAAGGTGAGCCAT
CTTTATGGATTTGGACTGATGGACGCAGAAGCCATGGTGATGGAGGCAGAGAAGTGGACC
ACCGTTCCCCGGCAGCACGTGTGTGTGGAGAGCACAGACCGACAAATCAAGACAATCCGC
CCTAACAGTGCAGTGCGCTCCATCTACAAAGCTTCAGGCTGCTCGGATAACCCCAACCGC
CATGTCAACTACCTGGAGCACGTCGTTGTGCGCATCACCATCACCCACCCCAGGAGAGGA
GACCTGGCCATCTACCTGACCTCGCCCTCTGGAACTAGGTCTCAGCTTTTGGCCAACAGG
CTATTTGATCACTCCATGGAAGGATTCAAAAACTGGGAGTTCATGACCATTCATTGCTGG
GGAGAAAGAGCTGCTGGTGACTGGGTCCTTGAAGTTTATGATACTCCCTCTCAGCTAAGG
AACTTTAAGACTCCAGGTAAATTGAAAGAATGGTCTTTGGTCCTCTACGGCACCTCCGTG
CAGCCATATTCACCAACCAATGAATTTCCGAAAGTGGAACGGTTCCGCTATAGCCGAGTT
GAAGACCCCACAGACGACTATGGCACAGAGGATTATGCAGGTCCCTGCGACCCTGAGTGC
AGTGAGGTTGGCTGTGACGGGCCAGGACCAGACCACTGCAATGACTGTTTGCACTACTAC
TACAAGCTGAAAAACAATACCAGGATCTGTGTCTCCAGCTGCCCCCCTGGCCACTACCAC
GCCGACAAGAAGCGCTGCAGGAAGTGTGCCCCCAACTGTGAGTCCTGCTTTGGGAGCCAT
GGTGACCAATGCATGTCCTGCAAATATGGATACTTTCTGAATGAAGAAACCAACAGCTGT
GTTACTCACTGCCCTGATGGGTCATATCAGGATACCAAGAAAAATCTTTGCCGGAAATGC
AGTGAAAACTGCAAGACATGTACTGAATTCCATAACTGTACAGAATGTAGGGATGGGTTA
AGCCTGCAGGGATCCCGGTGCTCTGTCTCCTGTGAAGATGGACGGTATTTCAACGGCCAG
GACTGCCAGCCCTGCCACCGCTTCTGCGCCACTTGTGCTGGGGCAGGAGCTGATGGGTGC
ATTAACTGCACAGAGGGCTACTTCATGGAGGATGGGAGATGCGTGCAGAGCTGTAGTATC
AGCTATTACTTTGACCACTCTTCAGAGAATGGATACAAATCCTGCAAAAAATGTGATATC
AGTTGTTTGACGTGCAATGGCCCAGGATTCAAGAACTGTACAAGCTGCCCTAGTGGGTAT
CTCTTAGACTTAGGAATGTGTCAAATGGGAGCCATTTGCAAGGATGCAACGGAAGAGTCC
TGGGCGGAAGGAGGCTTCTGTATGCTTGTGAAAAAGAACAATCTGTGCCAACGGAAGGTT
CTTCAACAACTTTGCTGCAAAACATGTACATTTTAAGGC

Protein sequence predicted based on nucleotide sequence

MGWGSRCCCPGRLDLLCVLALLGGCLLPVCRTRVYTNHWAVKIAGGFPEANRIASKYGFI
NIGQIGALKDYYHFYHSRTIKRSVISSRGTHSFISMEPKVEWIQQQVVKKRTKRDYDFSR
AQSTYFNDPKWPSMWYMHCSDNTHPCQSDMNIEGAWKRGYTGKNIVVTILDDGIERTHPD
LMQNYDALASCDVNGNDLDPMPRYDASNENKHGTRCAGEVAAAANNSHCTVGIAFNAKIG
GVRMLDGDVTDMVEAKSVSFNPQHVHIYSASWGPDDDGKTVDGPAPLTRQAFENGVRMGR
RGLGSVFVWASGNGGRSKDHCSCDGYTNSIYTISISSTAESGKKPWYLEECSSTLATTYS
SGESYDKKIITTDLRQRCTDNHTGTSASAPMAAGIIALALEANPFLTWRDVQHVIVRTSR
AGHLNANDWKTNAAGFKVSHLYGFGLMDAEAMVMEAEKWTTVPRQHVCVESTDRQIKTIR
PNSAVRSIYKASGCSDNPNRHVNYLEHVVVRITITHPRRGDLAIYLTSPSGTRSQLLANR
LFDHSMEGFKNWEFMTIHCWGERAAGDWVLEVYDTPSQLRNFKTPGKLKEWSLVLYGTSV
QPYSPTNEFPKVERFRYSRVEDPTDDYGTEDYAGPCDPECSEVGCDGPGPDHCNDCLHYY
YKLKNNTRICVSSCPPGHYHADKKRCRKCAPNCESCFGSHGDQCMSCKYGYFLNEETNSC
VTHCPDGSYQDTKKNLCRKCSENCKTCTEFHNCTECRDGLSLQGSRCSVSCEDGRYFNGQ
DCQPCHRFCATCAGAGADGCINCTEGYFMEDGRCVQSCSISYYFDHSSENGYKSCKKCDI
SCLTCNGPGFKNCTSCPSGYLLDLGMCQMGAICKDATEESWAEGGFCMLVKKNNLCQRKV
LQQLCCKTCTF*G

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
1 error; 1 non-synonomous errors;
 early stop codon
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable