PHLDA1 (Homo sapiens) in pLX304

Explanation of Terms

Gene:
Gene Symbol:  PHLDA1
Gene Name:  pleckstrin homology-like domain, family A, member 1
Original Clone ID:
ccsbBroad304_14068
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
PMID: 24954507
Title: PHLDA1 expression is controlled by an estrogen receptor-NFkappaB-miR-181 regulatory loop and is essential for formation of ER+ mammospheres
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00434827
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 778nts
Open reading frame: 1 to 778

ATGCTGGAGAGTAGCGGCNGCAAAGCGCTGAAGGAGGGCGTGCTGGAGAAGCGCAGCGAC
GGGTTGTTGCAGCTCTGGAAGAAAAAGTGTTGCATCCTCACCGAGGAAGGGCTGCTGCTT
ATCCCGCCCAAGCAGCTGCAACACCAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAA
CAACAGCCCGGGCAGGGGCCGGCCGAGCCGTCCCAACCCAGTGGCCCCGCTGTCGCCAGC
CTCGAGCCGCCGGTCAAGCTCAAGGAACTGCACTTCTCCAACATGAAGACCGTGGACTGT
GTGGAGCGCAAGGGCAAGTACATGTACTTCACTGTGGTGATGGCAGAGGGCAAGGAGATC
GACTTTCGGTGCCCGCAAGACCAGGGCTGGAACGCCGAGATCACGCTGCAGATGGTGCAG
TACAAGAATCGTCAGGCCATCCTGGCGGTCAAATCCACGCGGCAGAAGCAGCAGCACCTG
GTCCAGCAGCAGCCCCCCTCGCAGCCGCAGCCGCAGCCGCAGCTCCAGCCCCAACCCCAG
CCTCAGCCTCAGCCGCAACCCCAGCCCCAATCACAACCCCAGCCTCAGCCCCAACCCAAG
CCTCAGCCCCAGCAGCTCCACCCGTATCCGCATCCACATCCACATCCACACTCTCATCCT
CACTCGCACCCACACCCTCACCCGCACCCGCATCCGCACCAAATACCGCACCCACACCCA
CAGCCGCACTCGCAGCCGCACGGGCACCGGCTTCTCCGCAGCACCTCCAACTCTGCC

Protein sequence predicted based on nucleotide sequence

MLESSGKALKEGVLEKRSDGLLQLWKKKCCILTEEGLLLIPPKQLQHQQQQQQQQQQQQQ
QPGQGPAEPSQPSGPAVASLEPPVKLKELHFSNMKTVDCVERKGKYMYFTVVMAEGKEID
FRCPQDQGWNAEITLQMVQYKNRQAILAVKSTRQKQQHLVQQQPPSQPQPQPQLQPQPQP
QPQPQPQPQSQPQPQPQPKPQPQQLHPYPHPHPHPHSHPHSHPHPHPHPHPHQIPHPHPQ
PHSQPHGHRLLRSTSNSA

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
1 error; 1 non-synonomous errors;
 Clone mixture?
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable