RAPGEF5 (Homo sapiens) in pLX304

Explanation of Terms

Gene:
Gene Symbol:  RAPGEF5
Gene Name:  Rap guanine nucleotide exchange factor (GEF) 5
Original Clone ID:
ccsbBroad304_11412
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00435143
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1333nts
Open reading frame: 1 to 1333

ATGGGCAGCTCCCGGCTGAGGGTCTTTGACCCTCATTTGGAGAGGAAAGATTCCGCCGCG
GCGCTCTCAGACCGAGAGCTGCCCTTGCCTACCTTCGATGTGCCTTATTTCAAATACATC
GACGAGGAGGATGAGGACGATGAATGGAGCAGCCGCTCGCAGTCTTCCACCGAGGATGAC
TCAGTGGACTCTCTGCTCTCTGACAGATATGTGGTGGTGTCCGGGACCCCGGAGAAGATT
TTGGAGCACCTTTTGAATGACTTGCACCTGGAAGAAGTCCAGGACAAAGAAACAGAGACC
CTCCTGGATGACTTCCTTCTCACGTACACTGTCTTCATGACAACTGATGACTTGTGCCAG
GCTCTGTTAAGGCACTATTCTGCTAAGAAGTATCAAGGCAAAGAGGAAAACTCAGATGTT
CCGCGTAGGAAACGTAAAGTCTTGCATCTTGTTTCCCAGTGGATTGCTCTGTACAAAGAC
TGGTTACCTGAAGATGAACATTCAAAAATGTTTTTAAAGACCATATATAGGAATGTACTG
GATGATGTTTATGAATATCCAATACTTGAAAAAGAATTGAAAGAATTTCAAAAGATACTT
GGAATGCACCGTCGTCACACTGTAGATGAATATTCACCACAAAAAAAGAATAAAGCCCTT
TTCCACCAATTCAGTCTTAAGGAGAACTGGCTCCAGCATAGAGGAACTGTGACTGAAACG
GAGGAAATTTTCTGCCACGTGTATATAACAGAGCACTCCTATGTCAGTGTGAAGGCAAAA
GTTTCCAGTATAGCCCAAGAGATTCTGCTCTGCAGCCAGCTGGGCAAGCGAGTGCAGCTG
GTGAAAAAATTCATCAAAATTGCGGCTCACTGCAAAGCCCAGAGAAACCTGAATTCTTTC
TTTGCCATTGTGATGGGTCTCAACACTGCTTCTGTCAGTCGACTGTCGCAGACCTGGGAG
AAAATCCCTGGGAAGTTTAAGAAACTTTTCTCTGAACTTGAAAGTTTAACAGATCCTTCC
CTAAATCACAAAGCCTACAGAGATGCATTCAAAAAGATGAAGCCACCAAAAATCCCTTTC
GTGCCCTTATTGCTTAAAGATGTAACATTTATTCATGAAGGAAATAAAACTTTTTTGGAT
AATCTTGTCAATTTTGAAAAGCTGCATATGATCGCAGACACTGTCCGAACCCTGAGACAC
TGCAGGACTAACCAGTTTGGTGACCTGTCTCCAAAAGAGCATCAAGAGTTAAAGTCCTAT
GTTAATCACCTGTATGTCATTGACAGCCAGCAGGCTCTGTTTGAGCTCTCACACAGGATC
GAGCCGCGGGTG

Protein sequence predicted based on nucleotide sequence

MGSSRLRVFDPHLERKDSAAALSDRELPLPTFDVPYFKYIDEEDEDDEWSSRSQSSTEDD
SVDSLLSDRYVVVSGTPEKILEHLLNDLHLEEVQDKETETLLDDFLLTYTVFMTTDDLCQ
ALLRHYSAKKYQGKEENSDVPRRKRKVLHLVSQWIALYKDWLPEDEHSKMFLKTIYRNVL
DDVYEYPILEKELKEFQKILGMHRRHTVDEYSPQKKNKALFHQFSLKENWLQHRGTVTET
EEIFCHVYITEHSYVSVKAKVSSIAQEILLCSQLGKRVQLVKKFIKIAAHCKAQRNLNSF
FAIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHKAYRDAFKKMKPPKIPF
VPLLLKDVTFIHEGNKTFLDNLVNFEKLHMIADTVRTLRHCRTNQFGDLSPKEHQELKSY
VNHLYVIDSQQALFELSHRIEPRV

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
1 error
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable