AP2A1 (Homo sapiens) in pLX304

Explanation of Terms

Gene:
Gene Symbol:  AP2A1
Gene Name:  adaptor-related protein complex 2, alpha 1 subunit
Original Clone ID:
ccsbBroad304_14534
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00435615
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2950nts
Open reading frame: 1 to 2950

ATGCCGGCCGTGTCCAAGGGCGATGGGATGCGGGGGCTCGCGGTGTTCATCTCCGACATC
CGGAACTGTAAGAGCAAAGAGGCGGAAATTAAGAGAATCAACAAGGAACTGGCCAACATC
CGCTCCAAGTTCAAAGGAGACAAAGCCTTGGATGGCTACAGTAAGAAAAAATATGTGTGT
AAACTGCTTTTCATCTTCCTGCTTGGCCATGACATTGACTTTGGGCACATGGAGGCTGTG
AATCTGTTGAGTTCCAATAAATACACAGAGAAGCAAATAGGTTACCTGTTCATTTCTGTG
CTGGTGAACTCGAACTCGGAGCTGATCCGCCTCATCAACAACGCCATCAAGAATGACCTG
GCCAGCCGCAACCCCACCTTCATGTGCCTGGCCCTGCACTGCATCGCCAACGTGGGCAGC
CGGGAGATGGGCGAGGCCTTTGCCGCTGACATCCCCCGCATCCTGGTGGCCGGGGACAGC
ATGGACAGTGTCAAGCAGAGTGCGGCCCTGTGCCTCCTTCGACTGTACAAGGCCTCGCCT
GACCTGGTGCCCATGGGCGAGTGGACGGCGCGTGTGGTACACCTGCTCAATGACCAGCAC
ATGGGTGTGGTCACGGCCGCCGTCAGCCTCATCACCTGTCTCTGCAAGAAGAACCCAGAT
GACTTCAAGACGTGCGTCTCTCTGGCTGTGTCGCGCCTGAGCCGGATCGTCTCCTCTGCC
TCCACCGACCTCCAGGACTACACCTACTACTTCGTCCCAGCACCCTGGCTCTCGGTGAAG
CTCCTGCGGCTGCTGCAGTGCTACCCGCTTCCAGAGGATGCGGCTGTGAAGGGGCGGCTG
GTGGAATGTCTGGAGACTGTGCTCAACAAGGCCCAGGAGCCCCCCAAATCCAAGAAGGTG
CAGCATTCCAACGCCAAGAACGCCATCCTCTTCGAGACCATCAGCCTCATCATCCACTAT
GACAGTGAGCCCAACCTCCTGGTTCGGGCCTGCAACCAGCTGGGCCAGTTCCTGCAGCAC
CGGGAGACCAACCTGCGCTACCTGGCCCTGGAGAGCATGTGCACGCTGGCCAGCTCCGAG
TTCTCCCATGAAGCCGTCAAGACGCACATTGACACCGTCATCAATGCCCTCAAGACGGAG
CGGGACGTCAGCGTGCGGCAGCGGGCGGCTGACCTCCTCTACGCCATGTGTGACCGGAGC
AATGCCAAGCAGATCGTGTCGGAGATGCTGCGGTACCTGGAGACGGCAGACTACGCCATC
CGCGAGGAGATCGTCCTGAAGGTGGCCATCCTGGCCGAGAAGTACGCCGTGGACTACAGC
TGGTACGTGGACACCATCCTCAACCTCATCCGCATTGCGGGCGACTACGTGAGTGAGGAG
GTGTGGTACCGTGTGCTACAGATCGTCACCAACCGTGATGACGTCCAGGGCTATGCCGCC
AAGACCGTCTTTGAGGCGCTCCAGGCCCCTGCCTGTCACGAGAACATGGTGAAGGTTGGC
GGCTACATCCTTGGGGAGTTTGGGAACCTGATTGCTGGGGACCCCCGCTCCAGCCCCCCA
GTGCAGTTCTCCCTGCTCCACTCCAAGTTCCATCTGTGCAGCGTGGCCACGCGGGCGCTG
CTGCTGTCCACCTACATCAAGTTCATCAACCTCTTCCCCGAGACCAAGGCCACCATCCAG
NGGNGNT-----------------------------------------------------
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-----------------------------------------GCCCNCATCCAGGGGCTGT
GTATTATTG

Protein sequence predicted based on nucleotide sequence

MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVC
KLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDL
ASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASP
DLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSA
STDLQDYTYYFVPAPWLSVKLLRLLQCYPLPEDAAVKGRLVECLETVLNKAQEPPKSKKV
QHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSE
FSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAI
REEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAA
KTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRAL
LLSTYIKFINLFPETKATIQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXPSRGCVLL

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
>4 errors; >4 non-synonomous errors;
 Clone mixture?; insertion or deletion in coding sequence
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable