ASPM (Homo sapiens) in pLX304

Explanation of Terms

Gene:
Gene Symbol:  ASPM
Gene Name:  asp (abnormal spindle) homolog, microcephaly associated (Drosophila)
Original Clone ID:
ccsbBroad304_13488
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00436246
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3427nts
Open reading frame: 1 to 3427

ATGTCTCTCAGAGCTTATACTGCTCGGTGTAGGTTAAACAGACTACGTCGTGCAGCATGC
CGTTTGTTTACTTCTGAAAAAATGGTTAAAGCTATTAAAAAGCTTGAAATTGAAATTGAA
GCTAGGCGGTTAATTGTTCGAAAAGATAGACACCTATGGAAAGATGTGGGAGAACGTCAG
AAAGTCCTGAATTGGCTGTTGTCCTACAATCCTTTGTGGCTTCGAATTGGTCTAGAGACA
ACTTATGGAGAACTCATATCTTTGGAAGATAACAGTGATGTCACAGGGTTGGCTATGTTT
ATTCTGAATCGCCTACTTTGGAATCCTGATATAGCAGCTGAGTATAGACACCCCACTGTT
CCTCACCTGTATAGAGATGGTCATGAAGAAGCTTTGTCCAAGTTTACATTGAAAAAGTTA
TTGTTGTTGGTCTGTTTTCTTGATTATGCTAAAATTTCCAGACTCATTGATCATGATCCT
TGTCTCTTCTGTAAAGATGCCGAATTCAAGGCTAGTAAAGAAATCCTTTTGGCTTTTTCA
CGAGATTTCCTAAGTGGTGAAGGTGACCTTTCCCGTCACCTTGGCTTATTGGGATTACCT
GTTAACCATGTTCAGACACCATTTGATGAATTTGATTTTGCCGTTACAAATCTTGCCGTA
GACTTGCAATGTGGAGTGCGCCTTGTGCGAACCATGGAACTTCTCACACAGAACTGGGAC
CTCTCAAAGAAACTCAGGATTCCGGCAATAAGTCGTCTTCAAAAGATGCACAATGTTGAC
ATTGTTCTTCAAGTTCTTAAATCACGAGGAATTGAATTAAGTGATGAGCATGGAAATACA
ATTCTATCTAAGGATATTGTGGATAGGCACAGAGAAAAAACTCTCAGGTTGCTTTGGAAA
ATAGCGTTTGCTTTTCAGGTGGATATTTCCCTTAACTTAGATCAATTAAAGGAAGAAATT
GCCTTTCTAAAACACACAAAGAGTATAAAGAAAACAATATCTCTACTATCATGCCATTTT
GATGATCTTATTAATAAGAAAAAAGGCAAAAGGGATAGTGGTTCCTTTGAACAATATAGT
GAAAACATAAAGTTATTGATGGATTGGGTAAATGCTGTTTGTGCCTTCTATAATAAAAAG
GTGGAGAATTTTACAGTGTCTTTCTCAGACGGCCGTGTGTTATGTTACCTGATCCACCAT
TACCATCCTTGCTATGTGCCATTTGACGCTATATGTCAGCGTACTACTCAAACTGTGGAA
TGTACGCAAACTGGTTCAGTGGTATTAAATTCATCATCTGAATCTGATGACAGTTCTCTG
GATATGTCTCTTAAAGCATTTGATCATGAAAATACTTCAGAGCTATACAAAGAGCTCCTA
GAAAATGAAAAGAAAAATTTTCACTTGGTTAGGTCTGCAGTTAGAGACCTTGGTGGAATA
CCTGCTATGATTAATCATTCAGATATGTCAAATACAATTCCAGATGAAAAGGTGGTTATT
ACCTATTTGTCATTTCTTTGTGCAAGGCTTTTGGATCTTCGTAAAGAAATAAGAGCTGCT
CGACTCATACAAACAACATGGAGAAAATATAAACTAAAAACAGATCTCAAACGCCATCAG
GAGAGAGAGAAAGCTGCAAGAATTATTCAATTGGCTGTAATCAATTTTCTAGCAAAACAA
AGATTGAGAAAAAGAGTTAATGCAGCACTCGTCATTCAGAAATATTGGCGAAGAGTCTTA
GCACAGAGAAAATTATTAATGTTAAAAAAGGAAAAGCTGGAAAAAGTTCAAAATAAAGCA
GCATCACTTATTCAGGCTATGTGGAGGAGATATAGAGCCAAGAAATATTTATGTAAAGTG
AAAGCTGCCTGCAAGATTCAAGCCTGGTATAGATGTTGGAGAGCACACAAAGAATATCTA
GCTATATTAAAAGCTGTTAAAATTATTCAAGGTTGCTTCTATACCAAACTAGAGAGAACA
CGGTTTTTGAATGTGAGAGCATCAGCAATTATCATTCAGAGAAAATGGAGAGCTATACTT
CCTGCAAAGATAGCTCATGAACACTTCTTAATGATAAAAAGACATCGAGCTGCTTGTTTG
ATCCAAGCACATTATAGAGGATATAAAGGAAGGCAGGTCTTTCTTCGGCAGAAATCTGCT
GCTTTGATCATACAAAAATATATACGAGCCAGGGAGGCTGGAAAGCATGAAAGGATAAAA
TATATTGAATTTAAAAAATCTACAGTTATCCTACAAGCACTGGTGCGTGGTTGGCTAGTA
CGAAAAAGATTTTTAGAACAGAGAGCCAAAATTCGACTTCTTCACTTCACTGCAGCTGCA
TATTATCACCTGAATGCTGTTAGAATTCAAAGAGCCTATAAACTTTACCTGGCTGTGAAG
AATGCTAACAAGCAGGTTAATTCAGTCATCTGTATTCAGAGATGGTTTCGAGCAAGATTA
CAAGAAAAGAGATTTATTCAGAAATATCATAGCATCAAAAAGATTGAGCATGAAGGTCAA
GAATGTCTGAGCCAGCGAAATAGGGCTGCATCAGTAATACAGAAAGCAGTGCGCCATTTT
CTCCTCCGTAAAAAGCAGGAAAAATTCACTAGTGGAATCATTAAAATTCAGGCATTATGG
AGAGGCTATTCTTGGAGGAAGAAAAATGATTGTACAAAAATTAAAGCTATACGACTAAGT
CTTCAAGTTGTTAATAGGGAGATTCGAGAAGAAAACAAACTCTACAAAAGAACTGCACTT
GCACTTCATTACCTTTTGACATATAAGCACCTTTCTGCCATTCTTGAGGCCTTAAAACAC
CTAGAGGTAGTTACTAGATTGTCTCCACTTTGTTGTGAGAACATGGCCCAGAGTGGAGCA
ATTTCTAAAATATTTGTTTTGATCCGAAGTTGTAATCGCAGTATTCCTTGTATGGAAGTC
ATCAGATATGCTGTGCAAGTCTTGCTTAATGTATCTAAGTATGAGAAAACTACTTCAGCA
GTTTATGATGTAGAAAATTGTATAGATATACTATTGGAGCTTTTGCAGATATACCGAGAA
AAGCCTGGTAATAAAGTTGCAGACAAAGGCGGAAGCATTTTTACAAAAACTTGTTGTTTG
TTGGCTATTTTACTGAAGACAACAAATAGAGCCTCTGATGTACGAAGTAGGTCCAAAGTT
GTTGACCGTATTTACAGTCTCTACAAACTTACAGCTCATAAACATAAAATGAATACTGAA
AGAATACTTTACAAGCAAAAGAAGAATTCTTCTATAAGCATTCCTTTTATCCCAGAAACA
CCTGTAAGGACCAGAATAGTTTCAAGACTTAAGCCAGATTGGGTTTTGAGAAGAGATAAC
ATGGAAGAAATCACAAATCCCCTGCAAGCTATTCAAATGGTGATGGATACGCTTGGCATT
CCCTAT

Protein sequence predicted based on nucleotide sequence

MSLRAYTARCRLNRLRRAACRLFTSEKMVKAIKKLEIEIEARRLIVRKDRHLWKDVGERQ
KVLNWLLSYNPLWLRIGLETTYGELISLEDNSDVTGLAMFILNRLLWNPDIAAEYRHPTV
PHLYRDGHEEALSKFTLKKLLLLVCFLDYAKISRLIDHDPCLFCKDAEFKASKEILLAFS
RDFLSGEGDLSRHLGLLGLPVNHVQTPFDEFDFAVTNLAVDLQCGVRLVRTMELLTQNWD
LSKKLRIPAISRLQKMHNVDIVLQVLKSRGIELSDEHGNTILSKDIVDRHREKTLRLLWK
IAFAFQVDISLNLDQLKEEIAFLKHTKSIKKTISLLSCHFDDLINKKKGKRDSGSFEQYS
ENIKLLMDWVNAVCAFYNKKVENFTVSFSDGRVLCYLIHHYHPCYVPFDAICQRTTQTVE
CTQTGSVVLNSSSESDDSSLDMSLKAFDHENTSELYKELLENEKKNFHLVRSAVRDLGGI
PAMINHSDMSNTIPDEKVVITYLSFLCARLLDLRKEIRAARLIQTTWRKYKLKTDLKRHQ
EREKAARIIQLAVINFLAKQRLRKRVNAALVIQKYWRRVLAQRKLLMLKKEKLEKVQNKA
ASLIQAMWRRYRAKKYLCKVKAACKIQAWYRCWRAHKEYLAILKAVKIIQGCFYTKLERT
RFLNVRASAIIIQRKWRAILPAKIAHEHFLMIKRHRAACLIQAHYRGYKGRQVFLRQKSA
ALIIQKYIRAREAGKHERIKYIEFKKSTVILQALVRGWLVRKRFLEQRAKIRLLHFTAAA
YYHLNAVRIQRAYKLYLAVKNANKQVNSVICIQRWFRARLQEKRFIQKYHSIKKIEHEGQ
ECLSQRNRAASVIQKAVRHFLLRKKQEKFTSGIIKIQALWRGYSWRKKNDCTKIKAIRLS
LQVVNREIREENKLYKRTALALHYLLTYKHLSAILEALKHLEVVTRLSPLCCENMAQSGA
ISKIFVLIRSCNRSIPCMEVIRYAVQVLLNVSKYEKTTSAVYDVENCIDILLELLQIYRE
KPGNKVADKGGSIFTKTCCLLAILLKTTNRASDVRSRSKVVDRIYSLYKLTAHKHKMNTE
RILYKQKKNSSISIPFIPETPVRTRIVSRLKPDWVLRRDNMEEITNPLQAIQMVMDTLGI
PY

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
1 error
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable