SEMA3D (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  SEMA3D
Gene Name:  sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D
Original Clone ID:
FLH178108.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00043648
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1251nts
Open reading frame: 1 to 1251

ATGGCTGACATCAGAGCAGTTTTTAATGGTCCATATGCTCATAAGGAAAGTGCAGACCAT
CGTTGGGTGCAGTATGATGGGAGAATTCCTTATCCACGGCCTGGTACATGTCCAAGCAAA
ACCTATGACCCACTGATTAAGTCCACCCGAGATTTTCCAGATGATGTCATCAGTTTCATA
AAGCGGCACTCTGTGATGTATAAGTCCGTATACCCAGTTGCAGGAGGACCAACGTTCAAG
AGAATCAATGTGGATTACAGACTGACACAGATAGTGGTGGATCATGTCATTGCAGAAGAT
GGCCAGTACGATGTAATGTTTCTTGGAACAGACATTGGAACTGTCCTCAAAGTTGTCAGC
ATTTCAAAGGAAAAGTGGAATATGGAAGAGGTAGTGCTGGAGGAGTTGCAGATATTCAAG
CACTCATCAATCATCTTGAACATGGAATTGTCTCTGAAGCAGCAACAATTGTACATTGGT
TCCCGAGATGGATTGGTTCAGCTCTCCTTGCACAGATGCGACACTTATGGGAAAGCTTGC
GCAGACTGTTGTCTTGCCAGAGACCCCTACTGTGCCTGGGATGGAAATGCATGCTCTCGA
TATGCTCCTACTTCTAAAAGGAGAGCTAGACGCCAAGATGTAAAATATGGCGACCCAATC
ACCCAGTGCTGGGACATCGAAGACAGCATTAGTCATGAAACTGCTGATGAAAAGGTGATT
TTTGGCATTGAATTTAACTCAACCTTTCTGGAATGTATACCTAAATCCCAACAAGCAACT
ATTAAATGGTATATCCAGAGGTCAGGGGATGAGCATCGAGAGGAGTTGAAGCCCGATGAA
AGAATCATCAAAACGGAATATGGGCTACTGATTCGAAGTTTGCAGAAGAAGGATTCTGGG
ATGTATTACTGCAAAGCCCAGGAGCACACTTTCATCCACACCATAGTGAAGCTGACTTTG
AATGTCATTGAGAATGAACAGATGGAAAATACCCAGAGGGCAGAGCATGAGGAGGGGAAG
GTCAAGGATCTATTGGCTGAGTCACGGTTGAGATACAAAGACTACATCCAAATCCTTAGC
AGCCCAAACTTCAGCCTCGACCAGTACTGCGAACAGATGTGGCACAGGGAGAAGCGGAGA
CAGAGAAACAAGGGGGGCCCAAAGTGGAAGCACATGCAGGAAATGAAGAAGAAACGAAAT
CGAAGACATCACAGAGACCTGGATGAGCTCCCTAGAGCTGTAGCCACGTAG

Protein sequence predicted based on nucleotide sequence

MADIRAVFNGPYAHKESADHRWVQYDGRIPYPRPGTCPSKTYDPLIKSTRDFPDDVISFI
KRHSVMYKSVYPVAGGPTFKRINVDYRLTQIVVDHVIAEDGQYDVMFLGTDIGTVLKVVS
ISKEKWNMEEVVLEELQIFKHSSIILNMELSLKQQQLYIGSRDGLVQLSLHRCDTYGKAC
ADCCLARDPYCAWDGNACSRYAPTSKRRARRQDVKYGDPITQCWDIEDSISHETADEKVI
FGIEFNSTFLECIPKSQQATIKWYIQRSGDEHREELKPDERIIKTEYGLLIRSLQKKDSG
MYYCKAQEHTFIHTIVKLTLNVIENEQMENTQRAEHEEGKVKDLLAESRLRYKDYIQILS
SPNFSLDQYCEQMWHREKRRQRNKGGPKWKHMQEMKKKRNRRHHRDLDELPRAVAT*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1460
Start on reference sequence 210
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable