TOPBP1 (Homo sapiens) in pLX304

Explanation of Terms

Gene:
Gene Symbol:  TOPBP1
Gene Name:  topoisomerase (DNA) II binding protein 1
Original Clone ID:
ccsbBroad304_11589
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00436980
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 4306nts
Open reading frame: 1 to 4306

ATGCACCACCAGCGATGTGTCCCAAGAGCCGAACATCCAGTTTATAATATGGTTATGTCT
GATGTAACCATATCTTGTACAAGTCTGGAAAAAGAAAAAAGGGAAGAAGTTCATAAATAT
GTACAAATGATGGGCGGACGAGTATACAGAGACCTTAATGTATCAGTAACTCACCTTATT
GCAGGAGAAGTTGGTAGCAAAAAATATTTAGTTGCTGCAAACCTGAAGAAACCTATTTTG
CTTCCCTCTTGGATAAAAACACTTTGGGAGAAGTCACAAGAGAAAAAAATAACTAGATAT
ACTGATATAAACATGGAAGATTTCAAGTGTCCTATTTTTCTTGGTTGCATAATCTGTGTG
ACTGGCTTATGTGGCTTAGACAGGAAAGAAGTTCAGCAACTCACAGTTAAGCATGGAGGT
CAATACATGGGACAATTGAAAATGAATGAATGTACACACCTCATTGTGCAAGAACCAAAA
GGTCAGAAGTATGAGTGTGCCAAGAGATGGAATGTACACTGTGTGACCACACAGTGGTTT
TTTGACAGTATTGAGAAAGGTTTTTGTCAGGATGAATCCATATACAAGACAGAACCTAGA
CCAGAAGCAAAGACTATGCCCAATTCTTCAACTCCTACCAGCCAGATCAACACAATTGAT
AGTCGTACTCTTTCAGATGTCAGCAATATTTCCAACATAAATGCAAGTTGCGTAAGTGAA
TCAATATGTAATTCACTTAACAGCAAACTGGAGCCTACACTTGAAAATCTAGAAAATCTG
GATGTCAGTGCATTTCAAGCACCTGAAGATTTATTAGATGGTTGTCGGATATATCTTTGC
GGTTTTAGTGGCAGAAAGCTAGATAAACTGAGAAGACTTATTAACAGTGGAGGTGGAGTT
CGTTTTAACCAGCTAAATGAAGATGTAACTCATGTTATTGTGGGAGATTATGATGATGAA
TTGAAGCAGTTTTGGAATAAATCAGCCCACAGGCCTCATGTAGTGGGAGCAAAGTGGTTG
CTAGAGTGTTTCAGTAAAGGTTATATGCTTTCTGAAGAACCATATATCCATGCTAATTAC
CAGCCAGTGGAAATTCCAGTTTCACATAAGCCTGAAAGTAAAGCAGCTCTTTTAAAAAAG
AAGAACAGCAGCTTCTCTAAGAAAGACTTTGCTCCTAGTGAAAAGCATGAGCAAGCTGAT
GAAGATCTGCTCTCTCAATATGAAAATGGTAGCTCCACAGTAGTTGAGGCTAAGACGTCT
GAAGCCAGGCCCTTTAATGATTCTACTCATGCTGAGCCCTTGAATGATTCTACTCACATT
TCTTTGCAAGAAGAAAACCAGTCTTCTGTCAGTCATTGTGTCCCTGATGTTTCTACAATT
ACTGAAGAAGGCTTATTTAGCCAAAAGAGTTTCCTTGTTTTGGGTTTTAGTAATGAAAAT
GAATCTAACATCGCAAACATCATAAAAGAAAATGCTGGGAAAATCATGTCCCTTCTGAGC
AGAACTGTTGCGGATTATGCTGTGGTTCCTCTGCTGGGGTGTGAAGTGGAAGCCACTGTG
GGAGAAGTTGTTACAAATACATGGCTGGTTACTTGCATAGACTATCAGACTTTGTTTGAT
CCAAAGTCGAATCCTCTCTTCACACCAGTTCCAGTAATGACAGGAATGACTCCTTTAGAG
GATTGTGTTATTTCATTTAGCCAGTGTGCTGGAGCAGAAAAAGAGTCTTTAACATTCCTA
GCAAACCTCCTTGGAGCAAGTGTTCAAGAATACTTTGTTCGCAAATCCAATGCAAAGAAA
GGCATGTTTGCCAGTACTCATCTTATACTGAAAGAACGTGGTGGCTCTAAATATGAAGCT
GCAAAGAAGTGGAATTTACCTGCCGTTACTATAGCTTGGCTGTTGGAGACTGCTAGAACG
GGAAAGAGAGCAGACGAAAGCCATTTTCTGATTGAAAATTCAACTAAAGAAGAACGAAGT
TTGGAAACAGAAATAACAAATGGAATCAATCTAAATTCAGATACTGCAGAGCATCCTGGC
ACACGCCTGCAAACTCACAGAAAAACCGTCGTTACACCTTTAGATATGAACCGCTTTCAG
AGTAAAGCTTTCCGTGCTGTGGTCTCACAACATGCCAGACAGGTCGCAGCCTCCCCAGCA
GTAGGACAACCACTTCAGAAGGAGCCCTCGTTACACCTGGATACACCATCAAAATTCCTG
TCCAAGGACAAACTCTTCAAGCCTTCCTTTGATGTGAAGGATGCACTTGCAGCCTTGGAA
ACTCCAGGACGTCCCAGCCAACAGAAAAGGAAACCGAGTACGCCACTCTCAGAAGTTATT
GTCAAAAACTTGCAACTTGCTTTGGCAAATAGCTCTCGAAATGCTGTCGCTCTTTCTGCC
AGCCCTCAACTGAAAGAGGCCCAGTCAGAGAAGGAAGAAGCCCCAAAGCCACTTCACAAA
GTAGTGGTATGTGTTAGTAAAAAACTCAGTAAGAAGCAGAGTGAACTAAATGGGATCGCA
GCCTCTCTAGGAGCAGATTACAGGTGGAGTTTTGATGAAACAGTGACTCATTTCATCTAT
CAAGGGCGGCCAAATGACACTAATCGGGAGTATAAATCTGTAAAAGAAAGAGGAGTACAC
ATTGTTTCCGAGCACTGGCTTTTAGATTGTGCCCAAGAGTGTAAACATCTTCCTGAATCT
CTTTATCCACATACTTATAATCCCAAAATGAGCTTGGATATCAGCGCAGTGCAAGATGGC
CGGCTCTGTAATAGTCGACTACTCTCAGCTGTGTCTTCAACAAAGGATGATGAGCCAGAT
CCTTTGATTTTAGAAGAAAATGATGTAGACAATATGGCCACCAATAATAAAGAGTCAGCA
CCATCAAATGGAAGTGGAAAGAATGACTCTAAAGGAGTTCTGACACAGACCTTAGAGATG
AGAGAGAACTTTCAGAAGCAGTTACAGGAGATAATGTCTGCAACATCAATAGTGAAACCC
CAAGGGCAGAGGACTTCCCTTTCAAGAAGTGGTTGTAACAGCGCATCTTCAACCCCTGAC
AGCACTCGCTCTGCTCGCAGTGGACGAAGTAGAGTCCTAGAGGCACTGAGGCAGTCTCGT
CAGACAGTACCTGATGTCAACACAGAGCCTTCCCAAAATGAACAGATCATTTGGGATGAC
CCTACAGCAAGGGAGGAGAGAGCAAGGCTTGCCAGCAATTTGCAGTGGCCTAGTTGTCCC
ACACAATACTCTGAGCTTCAGGTTGACATTCAAAACTTGGAGGATTCTCCTTTTCAAAAG
CCTTTACATGATTCAGAAATTGCTAAACAGGCTGTCTGTGATCCTGGAAACATACGTGTG
ACTGAAGCTCCCAAACACCCAATCTCTGAAGAACTGGAAACTCCCATAAAAGACAGCCAC
CTGATCCCTACGCCTCAAGCCCCCAGTATTGCCTTTCCACTCGCCAACCCCCCTGTGGCT
CCGCACCCTAGAGAAAAGATTATAACGATAGAGGAGACTCATGAAGAATTAAAAAAACAG
TACATATTTCAGTTATCATCTCTGAATCCTCAAGAACGTATTGACTATTGTCATCTGATT
GAGAAACTAGGTGGATTGGTGATAGAAAAGCAGTGCTTTGATCCCACCTGTACACACATT
GTTGTGGGACATCCACTTCGAAACGAGAAGTATTTAGCCTCAGTGGCAGCTGGGAAGTGG
GTGCTTCATCGCTCCTACCTTGAAGCCTGCAGGACTGCTGGACACTTCGTGCAGGAAGAA
GACTATGAATGGGGAAGTAGTTCCATACTTGATGTTCTGACTGGAATCAATGTACAGCAA
CGAAGACTAGCACTTGCAGCAATGAGATGGAGAAAAAAAATCCAGCAAAGACAAGAATCT
GGCATTGTTGAGGGAGCATTTAGTGGGTGGAAGGTTATTTTACATGTGGATCAGTCTCGA
GAAGCAGGCTTCAAACGCCTTCTTCAGTCAGGAGGAGCAAAGGTGCTACCTGGTCATTCT
GTACCTTTATTTAAAGAGGCCACACATCTTTTTTCTGACTTGAATAAACTGAAACCAGAT
GACTCAGGAGTTAATATAGCAGAAGCTGCTGCCCAGAACGTGTACTGCTTGAGAACAGAA
TACATTGCTGATTATCTCATGCAGGAATCACCTCCTCATGTAGAAAATTACTGTCTACCA
GAAGCTATTTCATTTATTCAGAATAATAAGGAACTTGGGACTGGATTATCACAAAAGAGG
AAAGCTCCTACAGAAAAAAATAAAATCAAACGACCTAGAGTACAC

Protein sequence predicted based on nucleotide sequence

MHHQRCVPRAEHPVYNMVMSDVTISCTSLEKEKREEVHKYVQMMGGRVYRDLNVSVTHLI
AGEVGSKKYLVAANLKKPILLPSWIKTLWEKSQEKKITRYTDINMEDFKCPIFLGCIICV
TGLCGLDRKEVQQLTVKHGGQYMGQLKMNECTHLIVQEPKGQKYECAKRWNVHCVTTQWF
FDSIEKGFCQDESIYKTEPRPEAKTMPNSSTPTSQINTIDSRTLSDVSNISNINASCVSE
SICNSLNSKLEPTLENLENLDVSAFQAPEDLLDGCRIYLCGFSGRKLDKLRRLINSGGGV
RFNQLNEDVTHVIVGDYDDELKQFWNKSAHRPHVVGAKWLLECFSKGYMLSEEPYIHANY
QPVEIPVSHKPESKAALLKKKNSSFSKKDFAPSEKHEQADEDLLSQYENGSSTVVEAKTS
EARPFNDSTHAEPLNDSTHISLQEENQSSVSHCVPDVSTITEEGLFSQKSFLVLGFSNEN
ESNIANIIKENAGKIMSLLSRTVADYAVVPLLGCEVEATVGEVVTNTWLVTCIDYQTLFD
PKSNPLFTPVPVMTGMTPLEDCVISFSQCAGAEKESLTFLANLLGASVQEYFVRKSNAKK
GMFASTHLILKERGGSKYEAAKKWNLPAVTIAWLLETARTGKRADESHFLIENSTKEERS
LETEITNGINLNSDTAEHPGTRLQTHRKTVVTPLDMNRFQSKAFRAVVSQHARQVAASPA
VGQPLQKEPSLHLDTPSKFLSKDKLFKPSFDVKDALAALETPGRPSQQKRKPSTPLSEVI
VKNLQLALANSSRNAVALSASPQLKEAQSEKEEAPKPLHKVVVCVSKKLSKKQSELNGIA
ASLGADYRWSFDETVTHFIYQGRPNDTNREYKSVKERGVHIVSEHWLLDCAQECKHLPES
LYPHTYNPKMSLDISAVQDGRLCNSRLLSAVSSTKDDEPDPLILEENDVDNMATNNKESA
PSNGSGKNDSKGVLTQTLEMRENFQKQLQEIMSATSIVKPQGQRTSLSRSGCNSASSTPD
STRSARSGRSRVLEALRQSRQTVPDVNTEPSQNEQIIWDDPTAREERARLASNLQWPSCP
TQYSELQVDIQNLEDSPFQKPLHDSEIAKQAVCDPGNIRVTEAPKHPISEELETPIKDSH
LIPTPQAPSIAFPLANPPVAPHPREKIITIEETHEELKKQYIFQLSSLNPQERIDYCHLI
EKLGGLVIEKQCFDPTCTHIVVGHPLRNEKYLASVAAGKWVLHRSYLEACRTAGHFVQEE
DYEWGSSSILDVLTGINVQQRRLALAAMRWRKKIQQRQESGIVEGAFSGWKVILHVDQSR
EAGFKRLLQSGGAKVLPGHSVPLFKEATHLFSDLNKLKPDDSGVNIAEAAAQNVYCLRTE
YIADYLMQESPPHVENYCLPEAISFIQNNKELGTGLSQKRKAPTEKNKIKRPRVH

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable