Gene:
Gene Symbol:
IQGAP3
Gene Name:
IQ motif containing GTPase activating protein 3
Original Clone ID:
ccsbBroad304_09511
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00436986
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGAGGAGAGCAGCGGGCCCAGGCTGGGCAGCCTATGAACGCCTCACAGCTGAGGAG
ATGGATGAGCAGAGGCGGCAGAATGTTGCCTATCAGTACCTGTGCCGGCTGGAGGAGGCC
AAGCGCTGGATGGAGGCCTGCCTGAAGGAGGAGCTTCCTTCCCCGGTGGAGCTGGAGGAG
AGCCTTCGGAATGGAGTGCTGCTGGCCAAGCTGGGCCACTGTTTTGCACCCTCCGTGGTT
CCGTTGAAGAAGATCTACGATGTGGAGCAGCTGCGGTACCAGGCAACTGGCTTACATTTC
CGTCACACAGACAACATCAACTTTTGGCTATCTGCAATAGCCCACATCGGTCTGCCTTCG
ACCTTCTTCCCAGAGACCACGGACATCTATGACAAAAAGAACATGCCCCGGGTAGTCTAC
TGCATCCATGCTCTCAGTCTCTTCCTCTTCCGGCTGGGATTGGCCCCTCAGATACATGAT
CTATACGGGAAAGTGAAATTCACAGCTGAGGAACTCAGCAACATGGCGTCCGAACTGGCC
AAATATGGCCTCCAGCTGCCTGCCTTCAGCAAGATCGGGGGCATCTTGGCCAATGAGCTC
TCGGTGGATGAGGCTGCAGTCCATGCAGCTGTTCTTGCCATCAATGAAGCAGTGGAGCGA
GGGGTGGTGGAGGACACCCTGGCTGCCTTGCAGAATCCCAGTGCTCTTCTGGAGAATCTC
CGAGAGCCTCTGGCAGCCGTCTACCAGGAGATGCTGGCCCAGGCCAAGATGGAGAAGGCA
GCCAATGCCAGGAACCATGATGACAGAGAAAGCCAGGACATCTATGACCACTACCTAACT
CAGGCTGAAATCCAGGGCAATATCAACCATGTCAACGCCCATGGGGCTCTAGAAGTTGTT
GATGATGCCCTGGAAAGACAGAGCCCTGAAGCCTTGCTCAAGGCCCTTCAAGACCCTGCC
CTGGCCCTGCGAGGGGTGAGGAGAGACTTTGCTGACTGGTACCTGGAGCAGCTGAACTCA
GACAGAGAGCAGAAGGCACAGGAGCTGGGCCTGGTGGAGCTTCTGGAAAAGGAGGAAGTC
CAGGCTGGTGTGGCTGCAGCCAACACAAAGGGTGATCAGGAACAAGCCATGCTCCACGCT
GTGCAGCGGATCAACAAAGCCATCCGGAGGGGAGTGGCGGCTGACACTGTGAAGGAGCTG
ATGTGCCCTGAGGCCCAGCTGCCTCCAGTGTACCCTGTTGCATCGTCTATGTACCAGCTG
GAGCTGGCAGTGCTCCAGCAGCAGCAGGGGGAGCTTGGCCAGGAGGAGCTCTTCGTGGCT
GTGGAGATGCTCTCAGCTGTGGTCCTGATTAACCGGGCCCTGGAGGCCCGGGATGCCAGT
GGCTTCTGGAGCAGCCTGGTGAACCCTGCCACAGGCCTGGCTGAGGTGGAAGGAGAAAAT
GCCCAGCGTTACTTCGATGCCCTGCTGAAATTGCGACAGGAGCGTGGGATGGGTGAGGAC
TTCCTGAGCTGGAATGACCTGCAGGCCACCGTGAGCCAGGTCAATGCACAGACCCAGGAA
GAGACTGACCGGGTCCTTGCAGTCAGCCTCATCAATGAGGCTCTGGACAAAGGCAGCCCT
GAGAAGACTCTGTCTGCCCTACTGCTTCCTGCAGCTGGCCTAGATGATGTCAGCCTCCCT
GTCGCCCCTCGGTACCATCTCCTCCTTGTGGCAGCCAAAAGGCAGAAGGCCCAGGTGACA
GGGGATCCTGGAGCTGTGCTGTGGCTTGAGGAGATCCGCCAGGGAGTGGTCAGAGCCAAC
CAGGACACTAATACAGCTCAGAGAATGGCTCTTGGTGTGGCTGCCATCAATCAAGCCATC
AAGGAGGGCAAGGCAGCCCAGACTGAGCGGGTGTTGAGGAACCCCGCAGTGGCCCTTCGA
GGGGTAGTTCCCGACTGTGCCAACGGCTACCAGCGAGCCCTGGAAAGTGCCATGGCAAAG
AAACAGTGTCCAGCAGACACAGCTTTCTGGGTTCAACATGACATGAAGGATGGCACTGCC
TACTACTTCCATCTGCAGACCTTCCAGGGGATCTGGGAGCAACCTCCTGGCTGCCCCCTC
AACACCTCTCACCTGACCCGGGAGGAGATCCAGTCAGCTGTCACCAAGGTCACTGCTGCC
TATGACCGCCAACAGCTCTGGAAAGCCAACGTCGGCTTTGTTATCCAGCTCCAGGCCCGC
CTCCGTGGCTTCCTAGTTCGGCAGAAGTTTGCTGAGCATTCCCACTTTCTGAGGACCTGG
CTCCCAGCAGTCATCAAGATCCAGGCTCATTGGCGGGGTTATAGGCAGCGGAAGATTTAC
CTGGAGTGGTTGCAGTATTTTAAAGCAAACCTGGATGCCATAATCAAGATCCAGGCCTGG
GCCCGGATGTGGGCAGCTCGGAGGCAATACCTGAGGCGTCTGCACTACTTCCAGAAGAAT
GTTAACTCCATTGTGAAGATCCAGGCATTTTTCCGAGCCAGGAAAGCCCAAGATGACTAC
AGGATATTAGTGCATGCACCCCACCCTCCTCTCAGTGTGGTACGCAGATTTGCCCATCTC
TTGAATCAAAGCCAGCAAGACTTCTTGGCTGAGGCAGAGCTGCTGAAGCTCCAGGAAGAG
GTAGTTAGGAAGATCCGATCCAATCAGCAGCTGGAGCAGGACCTCAACATCATGGACATC
AAGATTGGCCTGCTGGTGAAGAACCGGATCACTCTGCAGGAAGTGGTCTCCCACTGCAAG
AAGCTGACCAAGAGGAATAAGGAACAGCTGTCAGATATGATGGTTCTGGACAAGCAGAAG
GGTTTAAAGTCGCTGAGCAAAGAGAAACGGCAGAAACTAGAAGCATACCAACACCTCTTC
TACCTGCTCCAGACTCAGCCCATCTACCTGGCCAAGCTGATCTTTCAGATGCCACAGAAC
AAAACCACCAAGTTCATGGAGGCAGTGATTTTCAGCCTGTACAACTATGCCTCCAGCCGC
CGAGAGGCCTATCTCCTGCTCCAGCTGTTCAAGACAGCACTCCAGGAGGAAATCAAGTCA
AAGGTGGAGCAGCCCCAGGACGTGGTGACAGGCAACCCAACAGTGGTGAGGCTGGTGGTG
AGATTCTACCGTAATGGGCGGGGACAGAGTGCCCTGCAGGAGATTCTGGGCAAGGTTATC
CAGGATGTGCTAGAAGACAAAGTGCTCAGCGTCCACACAGACCCTGTCCACCTCTATAAG
AACTGGATCAACCAGACTGAGGCCCAGACAGGGCAGCGCAGCCATCTCCCATATGATGTC
ACCCCGGAGCAGGCCTTGAGCCACCCCGAGGTCCAGAGACGACTGGACATCGCCCTACGC
AACCTCCTCGCCATGACTGATAAGTTCCTTTTAGCCATCACCTCATCTGTGGACCAAATT
CCGTATGGGATGCGATATGTGGCCAAAGTCCTGAAGGCAACTCTGGCAGAGAAATTCCCT
GACGCCACAGACAGCGAGGTCTATAAGGTGGTCGGGAACCTCCTGTACTACCGCTTCCTG
AACCCAGCTGTGGTGGCTCCTGACGCCTTCGACATTGTGGCCATGGCAGCTGGTGGAGCC
CTGGCTGCCCCCCAGCGCCATGCCCTGGGGGCTGTGGCTCAGCTCCTACAGCACGCTGCG
GCTGGCAAGGCCTTCTCTGGGCAGAGCCAGCACCTACGGGTCCTGAATGACTATCTGGAG
GAAACACACCTCAAGTTCAGGAAGTTCATCCATAGAGCCTGCCAGGTGCCAGAGCCAGAG
GAGCGTTTTGCAGTGGACGAGTACTCAGACATGGTGGCTGTGGCCAAACCCATGGTGTAC
ATCACCGTGGGGGAGCTGGTCAACACGCACAGGCTGTTGCTGGAGCACCAGGACTGCATT
GCCCCTGATCACCAAGACCCCCTGCATGAGCTCCTGGAGGATCTTGGGGAGCTGCCCACC
ATCCCTGACCTTATTGGTGAGAGCATCGCTGCAGATGGGCACACGGACCTGAGCAAGCTA
GAAGTGTCCCTGACGCTGACCAACAAGTTTGAAGGACTAGAGGCAGATGCTGATGACTCC
AACACCCGTAGCCTGCTTCTGAGCACCAAGCAGCTGTTGGCCGATATCATACAGTTCCAT
CCTGGGGACACCCTCAAGGAGATCCTGTCCCTCTCGGCTTCCAGAGAGCAAGAAGCAGCC
CACAAGCAGCTGATGAGCCGACGCCAGGCCTGTACAGCCCAGACACCGGAGCCACTGCGA
CGACACCGCTCACTGACAGCTCACTCCCTCCTGCCACTGGCAGAGAAGCAGCGGCGCGTC
CTGCGGAACCTGCGCCGACTTGAAGCCCTGGGGTTGGTCAGCGCCAGAAATGGCTACCAG
GGGCTAGTGGACGAGCTGGCCAAGGACATCCGCAACCAGCACAGACACAGGCACAGGCGG
AAGGCAGAGCTGGTGAAGCTGCAGGCCACATTACAGGGCCTGAGCACTAAGACCACCTTC
TATGAGGAGCAGGGTGACTACTACAGCCAGTACATCCGGGCCTGCCTGGACCACCTGGCC
CCCGACTCCAAGAGTTCTGGGAAGGGGAAGAAGCAGCCTTCTCTTCATTACACTGCTGCT
CAGCTCCTGGAAAAGGGTGTCTTGGTGGAAATTGAAGATCTTCCCGCCTCTCACTTCAGA
AACGTCATCTTTGACATCACGCCGGGAGATGAGGCAGGAAAGTTTGAAGTAAATGCCAAG
TTCCTGGGTGTGGACATGGAGCGATTTCAGCTTCACTATCAGGATCTCCTGCAGCTCCAG
TATGAGGGTGTGGCTGTCATGAAACTCTTCAACAAGGCCAAAGTCAATGTCAACCTTCTC
ATCTTCCTCCTCAACAAGAAGTTTTTGCGGAAG
Protein sequence predicted based on nucleotide sequence
MERRAAGPGWAAYERLTAEEMDEQRRQNVAYQYLCRLEEAKRWMEACLKEELPSPVELEE SLRNGVLLAKLGHCFAPSVVPLKKIYDVEQLRYQATGLHFRHTDNINFWLSAIAHIGLPS TFFPETTDIYDKKNMPRVVYCIHALSLFLFRLGLAPQIHDLYGKVKFTAEELSNMASELA KYGLQLPAFSKIGGILANELSVDEAAVHAAVLAINEAVERGVVEDTLAALQNPSALLENL REPLAAVYQEMLAQAKMEKAANARNHDDRESQDIYDHYLTQAEIQGNINHVNAHGALEVV DDALERQSPEALLKALQDPALALRGVRRDFADWYLEQLNSDREQKAQELGLVELLEKEEV QAGVAAANTKGDQEQAMLHAVQRINKAIRRGVAADTVKELMCPEAQLPPVYPVASSMYQL ELAVLQQQQGELGQEELFVAVEMLSAVVLINRALEARDASGFWSSLVNPATGLAEVEGEN AQRYFDALLKLRQERGMGEDFLSWNDLQATVSQVNAQTQEETDRVLAVSLINEALDKGSP EKTLSALLLPAAGLDDVSLPVAPRYHLLLVAAKRQKAQVTGDPGAVLWLEEIRQGVVRAN QDTNTAQRMALGVAAINQAIKEGKAAQTERVLRNPAVALRGVVPDCANGYQRALESAMAK KQCPADTAFWVQHDMKDGTAYYFHLQTFQGIWEQPPGCPLNTSHLTREEIQSAVTKVTAA YDRQQLWKANVGFVIQLQARLRGFLVRQKFAEHSHFLRTWLPAVIKIQAHWRGYRQRKIY LEWLQYFKANLDAIIKIQAWARMWAARRQYLRRLHYFQKNVNSIVKIQAFFRARKAQDDY RILVHAPHPPLSVVRRFAHLLNQSQQDFLAEAELLKLQEEVVRKIRSNQQLEQDLNIMDI KIGLLVKNRITLQEVVSHCKKLTKRNKEQLSDMMVLDKQKGLKSLSKEKRQKLEAYQHLF YLLQTQPIYLAKLIFQMPQNKTTKFMEAVIFSLYNYASSRREAYLLLQLFKTALQEEIKS KVEQPQDVVTGNPTVVRLVVRFYRNGRGQSALQEILGKVIQDVLEDKVLSVHTDPVHLYK NWINQTEAQTGQRSHLPYDVTPEQALSHPEVQRRLDIALRNLLAMTDKFLLAITSSVDQI PYGMRYVAKVLKATLAEKFPDATDSEVYKVVGNLLYYRFLNPAVVAPDAFDIVAMAAGGA LAAPQRHALGAVAQLLQHAAAGKAFSGQSQHLRVLNDYLEETHLKFRKFIHRACQVPEPE ERFAVDEYSDMVAVAKPMVYITVGELVNTHRLLLEHQDCIAPDHQDPLHELLEDLGELPT IPDLIGESIAADGHTDLSKLEVSLTLTNKFEGLEADADDSNTRSLLLSTKQLLADIIQFH PGDTLKEILSLSASREQEAAHKQLMSRRQACTAQTPEPLRRHRSLTAHSLLPLAEKQRRV LRNLRRLEALGLVSARNGYQGLVDELAKDIRNQHRHRHRRKAELVKLQATLQGLSTKTTF YEEQGDYYSQYIRACLDHLAPDSKSSGKGKKQPSLHYTAAQLLEKGVLVEIEDLPASHFR NVIFDITPGDEAGKFEVNAKFLGVDMERFQLHYQDLLQLQYEGVAVMKLFNKAKVNVNLL IFLLNKKFLRK
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: mammalian Marker: blasticidin
Host Type: mammalian Marker: blasticidin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLX304
Synonyms:
''
Sequencing Primer:
Forward:
CMV forward
Reverse:
None
Description:
with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;
Comments:
None
Size (bp):
9377
Parent Vector:
None
Properties:
Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name:
David Root
Publications:
PMID:
21706014
Title: A public genome-scale lentiviral expression library of human ORFs
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
polypurine tract
cPPT
human immunodeficiency virus type 1 central polypurine tract
3027
3042
promoter
CMV
CMV promoter
14
566
promoter
T7
T7 promoter
3690
3672
recombination site
attR1
595
719
recombination site
attR2
2282
2297
selectable marker
BlastR
Blasticidin resistance gene
8753
9148
selectable marker
AmpR
Ampicillin resistance gene
4444
5304
selectable marker
CAT
CAT/CamR Chloramphenical resistance
828
1487
selectable marker
ccdB
Control of cell death B gene
1829
2134
tag
V5
C-terminal V5 tag
2301
2342
trxn regulatory element
WPRE
woodchuck hepatitis post-transcriptional regulatory element
2386
2961
