PLEKHG3 (Homo sapiens) in pLX304

Explanation of Terms

Gene:
Gene Symbol:  PLEKHG3
Gene Name:  pleckstrin homology domain containing, family G (with RhoGef domain) member 3
Original Clone ID:
ccsbBroad304_11801
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00436994
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3658nts
Open reading frame: 1 to 3658

ATGCCTGTGTCCACCTCCCTCCACCAGGATGGCAGCCAGGAGCGGCCGGTGAGCCTGACC
TCTACCACCTCCTCGTCGGGCTCCTCCTGTGACAGTCGCAGTGCCATGGAGGAGCCCAGC
AGCTCCGAGGCTCCCGCCAAGAATGGGGCAGGCTCCCTGAGAAGCCGGCATCTGCCCAAC
AGCAACAACAACTCCAGCAGCTGGTTGAACGTGAAGGGGCCCCTCTCCCCGTTCAACAGC
CGGGCAGCGGCAGGGCCTGCACACCACAAGCTCAGCTACCTGGGCCGAGTGGTGCGGGAG
ATCGTGGAGACAGAGCGCATGTACGTACAGGACCTGCGCAGCATCGTGGAGGACTACCTC
TTGAAGATCATTGACACACCCGGGCTGCTGAAGCCAGAACAGGTCAGCGCCCTCTTTGGG
AACATAGAAAACATCTACGCGCTGAACAGCCAGCTCCTCAGAGACCTGGACAGCTGCAAT
AGTGACCCCGTGGCTGTGGCCAGCTGCTTTGTGGAAAGGAGCCAAGAGTTTGATATCTAC
ACTCAGTATTGCAACAATTACCCCAACTCCGTGGCCGCCCTGACGGAATGCATGCGGGAC
AAGCAGCAGGCCAAGTTCTTTCGGGACCGGCAGGAGCTGCTACAGCACTCGCTGCCCTTG
GGCTCCTACCTGCTGAAGCCAGTCCAGCGCATCCTCAAGTACCACCTGCTGCTCCAGGAA
ATTGCCAAACATTTTGATGAAGAAGAGGATGGCTTTGAGGTGGTGGAGGATGCCATTGAC
ACCATGACCTGTGTGGCCTGGTACATCAACGACATGAAGAGGAGGCATGAGCACGCGGTC
CGGCTCCAGGAGATTCAGTCACTCCTCATCAACTGGAAGGGGCCCGACCTGACCACCTAC
GGGGAGCTTGTCCTGGAGGGCACATTCCGCGTGCATCGCGTGCGCAATGAAAGGACCTTT
TTCCTCTTTGACAAAACACTGCTTATCACCAAGAAGCGGGGCGATCACTTTGTCTACAAG
GGCAACATCCCGTGCTCCTCCCTGATGCTGATCGAAAGCACCAGAGACTCCCTGTGCTTC
ACTGTCACCCACTACAAGCACAGCAAGCAGCAGTACAGCATCCAGGCCAAGACAGTGGAG
GAGAAACGGAACTGGACTCACCACATCAAGAGGCTCATCCTAGAGAACCACCATGCCACC
ATTCCCCAGAAGGCCAAGGAAGCCATCTTGGAAATGGATTCCTATTATCCCAATCGGTAC
CGCTGCAGCCCAGAGCGGCTGAAGAAGGCTTGGTCCTCCCAGGATGAGGTGTCCACCAAT
GTGCGCCAGGGGCGCCGGCAATCTGAGCCAACCAAACACCTGCTCAGGCAACTCAACGAG
AAAGCCCGAGCAGCAGGAATGAAGGGAAAGGGGCGCAGGGAGTCTGAAAGCTCCAGGAGC
AGCAGAAGGCCCAGTGGCCGGTCTCCAACCAGTACTGAGAAGCGCATGAGCTTCGAGTCC
ATTTCTTCCCTGCCAGAGGTTGAGCCGGACCCTGAGGCTGGGAGTGAGCAAGAGGTATTT
TCTGCTGTGGAAGGGCCCAGTGCCGAGGAGACGCCTTCAGACACAGAATCTCCAGAAGTC
CTGGAGACACAGCTTGATGCCCACCAGGGCCTTCTGGGGATGGACCCCCCAGGTGACATG
GTGGACTTCGTGGCAGCTGAGAGCACTGAGGACCTTAAGGCCCTGAGCAGCGAGGAGGAA
GAAGAAATGGGAGGTGCCGCCCAGGAGCCTGAGAGCCTTCTGCCACCCTCCGTGCTGGAC
CAGGCCAGCGTCATTGCGGAGCGATTTGTCAGCAGCTTCTCTCGGCGGAGCAGCGTGGCA
CAGGAGGACAGCAAGTCCAGTGGCTTTGGGAGCCCGCGGCTGGTCAGCCGGAGCAGCAGC
GTGCTCAGCCTGGAGGGCAGCGAGAAGGGCCTGGCCCGGCATGGCAGTGCCACAGACTCC
CTCAGCTGTCAGCTCTCCCCAGAAGTGGACATCAGTGTGGGGGTGGCCACAGAGGACAGC
CCTTCTGTCAATGGGATGGAGCCCCCAAGCCCAGGCTGCCCAGTGGAGCCTGACCGGTCT
TCCTGCAAGAAGAAGGAATCAGCACTCTCCACCCGAGACCGGCTGTTGCTAGACAAGATT
AAGAGCTATTATGAAAATGCAGAACACCATGATGCAGGCTTCAGCGTCCGTCGCCGGGAG
AGCCTCTCCTACATCCCCAAAGGACTGGTAAGAAACTCCATCTCCAGGTTCAACAGCCTT
CCCCGGCCAGACCCAGAGCCAGTACCTCCAGTGGGGAGCAAGAGACAGGTGGGCTCCCGG
CCGACTTCGTGGGCCCTGTTTGAGCTCCCAGGACCAAGCCAGGCAGTCAAAGGGGACCCA
CCTCCCATCTCAGATGCTGAGTTCCGCCCATCTTCAGAAATTGTGAAGATCTGGGAGGGA
ATGGAGTCTTCCGGAGGGAGCCCTGGGAAGGGGCCAGGCCAGGGCCAGGCCAATGGCTTT
GACCTGCATGAGCCACTCTTCATCCTGGAGGAGCATGAGCTGGGAGCCATCACAGAGGAG
TCGGCCACTGCCTCCCCGGAAAGCTCCTCTCCCACTGAGGGGCGCAGCCCGGCCCACCTG
GCCCGGGAGCTGAAAGAGCTGGTGAAGGAGCTGAGCAGCAGTACCCAGGGGGAGCTGGTG
GCCCCACTGCACCCCCGCATCGTGCAGCTCTCCCACGTAATGGACAGCCACGTGAGCGAG
CGCGTCAAGAACAAGGTCTACCAGCTGGCCCGCCAGTACAGCCTCCGGATCAAGAGCAAC
AAGCCAGTGATGGCCAGGCCACCACTGCAGTGGGAAAAGGTGGCCCCTGAGAGGGATGGG
AAGAGCCCCACTGTGCCCTGTCTACAGGAAGAGGCTGGAGAGCCATTAGGTGGCAAAGGT
AAGAGGAAGCCGGTGCTGTCTCTATTTGACTATGAGCAGCTGATGGCCCAGGAGCACAGC
CCTCCCAAGCCCTCCTCGGCTGGGGAGATGTCACCACAGCGTTTCTTCTTCAACCCGTCT
GCTGTCAGCCAGAGGACCACCTCGCCTGGGGGCCGGCCCTCCGCCCGGAGCCCCCTCAGC
CCCACAGAGACCTTCAGCTGGCCCGACGTCCGTGAGCTCTGCTCCAAGTATGCCTCCCGC
GATGAGGCACGCCGAGCAGGGGGCGGCCGGCCCCGCGGCCCACCCGTCAACAGGAGCCAC
TCGGTGCCGGAGAACATGGTAGAGCCACCTCTGTCGGGCAGGGTGGGCCGCTGCCGCAGC
CTGAGCACCAAGAGGGGCCGGGGAGGCGGAGAGGCTGCCCAATCCCCTGGGCCTCTGCCC
CAGAGCAAGCCGGATGGAGGCGAGACCCTGTATGTCACTGCAGACCTCACCCTGGAGGAC
AACCGGCGGGTGATTGTCATGGAGAAGGGACCCCTTCCCAGCCCCACTGCAGGGCTGGAG
GAGAGCAGTGGCCAGGGACCAAGCTCACCGGTGGCCCTGCTGGGGCAGGTTCAGGACTTC
CAGCAGTCTGCAGAGTGCCAGCCGAAGGAAGAGGGTTCCAGGGACCCGGCAGACCCGAGC
CAGCAGGGCAGAGTGAGAAACCTTAGAGAGAAGTTCCAGGCCTTGAACTCTGTCGGT

Protein sequence predicted based on nucleotide sequence

MPVSTSLHQDGSQERPVSLTSTTSSSGSSCDSRSAMEEPSSSEAPAKNGAGSLRSRHLPN
SNNNSSSWLNVKGPLSPFNSRAAAGPAHHKLSYLGRVVREIVETERMYVQDLRSIVEDYL
LKIIDTPGLLKPEQVSALFGNIENIYALNSQLLRDLDSCNSDPVAVASCFVERSQEFDIY
TQYCNNYPNSVAALTECMRDKQQAKFFRDRQELLQHSLPLGSYLLKPVQRILKYHLLLQE
IAKHFDEEEDGFEVVEDAIDTMTCVAWYINDMKRRHEHAVRLQEIQSLLINWKGPDLTTY
GELVLEGTFRVHRVRNERTFFLFDKTLLITKKRGDHFVYKGNIPCSSLMLIESTRDSLCF
TVTHYKHSKQQYSIQAKTVEEKRNWTHHIKRLILENHHATIPQKAKEAILEMDSYYPNRY
RCSPERLKKAWSSQDEVSTNVRQGRRQSEPTKHLLRQLNEKARAAGMKGKGRRESESSRS
SRRPSGRSPTSTEKRMSFESISSLPEVEPDPEAGSEQEVFSAVEGPSAEETPSDTESPEV
LETQLDAHQGLLGMDPPGDMVDFVAAESTEDLKALSSEEEEEMGGAAQEPESLLPPSVLD
QASVIAERFVSSFSRRSSVAQEDSKSSGFGSPRLVSRSSSVLSLEGSEKGLARHGSATDS
LSCQLSPEVDISVGVATEDSPSVNGMEPPSPGCPVEPDRSSCKKKESALSTRDRLLLDKI
KSYYENAEHHDAGFSVRRRESLSYIPKGLVRNSISRFNSLPRPDPEPVPPVGSKRQVGSR
PTSWALFELPGPSQAVKGDPPPISDAEFRPSSEIVKIWEGMESSGGSPGKGPGQGQANGF
DLHEPLFILEEHELGAITEESATASPESSSPTEGRSPAHLARELKELVKELSSSTQGELV
APLHPRIVQLSHVMDSHVSERVKNKVYQLARQYSLRIKSNKPVMARPPLQWEKVAPERDG
KSPTVPCLQEEAGEPLGGKGKRKPVLSLFDYEQLMAQEHSPPKPSSAGEMSPQRFFFNPS
AVSQRTTSPGGRPSARSPLSPTETFSWPDVRELCSKYASRDEARRAGGGRPRGPPVNRSH
SVPENMVEPPLSGRVGRCRSLSTKRGRGGGEAAQSPGPLPQSKPDGGETLYVTADLTLED
NRRVIVMEKGPLPSPTAGLEESSGQGPSSPVALLGQVQDFQQSAECQPKEEGSRDPADPS
QQGRVRNLREKFQALNSVG

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable