LNPEP (Homo sapiens) in pLX304

Explanation of Terms

Gene:
Gene Symbol:  LNPEP
Gene Name:  leucyl/cystinyl aminopeptidase
Original Clone ID:
ccsbBroad304_06534
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00437002
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3034nts
Open reading frame: 1 to 3034

ATGATTGAAAACAGCATGTTTGAGGAAGAACCAGATGTGGTGGATTTAGCCAAAGAGCCT
TGTTTACATCCTCTAGAGCCTGATGAGGTGGAATATGAGCCCCGGGGTTCCCGACTGCTG
GTGCGGGGTCTTGGTGAGCATGAGATGGAGGAGGATGAAGAGGATTATGAGTCATCAGCA
AAGCTGCTGGGCATGTCCTTCATGAATAGAAGCTCAGGCCTTCGGAACAGTGCAACTGGT
TACAGGCAGAGCCCAGATGGGGCTTGTTCAGTACCCTCTGCAAGGACCATGGTGGTCTGT
GCTTTTGTCATCGTGGTTGCTGTTTCTGTAATCATGGTGATTTACTTACTGCCCAGATGT
ACCTTTACCAAAGAAGGCTGCCATAAAAAAAACCAGTCAATTGGACTAATTCAGCCATTT
GCAACAAATGGGAAATTGTTTCCATGGGCACAGATCAGGCTTCCCACTGCCGTTGTGCCA
CTACGCTATGAACTCAGCCTACACCCGAACCTAACCTCGATGACATTCAGGGGTTCTGTG
ACAATTTCAGTTCAGGCTCTTCAGGTCACATGGAATATCATTCTTCATAGCACAGGTCAT
AATATTTCAAGAGTGACCTTTATGTCAGCAGTTTCAAGCCAAGAAAAACAAGCTGAGATC
CTGGAATATGCATATCATGGACAGATCGCCATTGTTGCCCCCGAAGCCCTTCTAGCAGGG
CACAATTATACGTTGAAGATAGAGTACTCGGCAAATATATCTAGTTCTTATTATGGGTTT
TATGGCTTCTCCTACACAGATGAAAGTAATGAGAAAAAGTACTTTGCAGCAACTCAGTTT
GAACCCCTGGCAGCAAGATCTGCTTTTCCTTGTTTTGATGAACCAGCATTTAAAGCCACT
TTTATCATCAAGATCATAAGGGATGAGCAATACACCGCTTTATCAAATATGCCTAAGAAG
TCATCAGTCGTTCTAGATGATGGACTTGTTCAGGATGAGTTTTCTGAGAGTGTGAAGATG
AGCACTTACTTGGTTGCTTTCATTGTGGGAGAGATGAAGAACCTGAGTCAGGACGTAAAT
GGAACCCTGGTTTCTATATATGCTGTACCAGAAAAGATTGGTCAAGTTCATTATGCCTTG
GAAACAACTGTGAAGCTTCTTGAGTTTTTTCAAAACTACTTTGAAATTCAGTACCCACTT
AAGAAATTGGATTTGGTGGCTATTCCTGACTTTGAAGCAGGAGCAATGGAAAATTGGGGT
TTGCTCGCCTTCCGAGAGGAGACACTTCTGTATGACAGTAACACTTCTTCAATGGCGGAT
AGAAAGCTGGTGACTAAAATCATTGCTCATGAGCTGGCCCACCAGTGGTTTGGCAATCTG
GTAACAATGAAGTGGTGGAATGACCTATGGCTAAATGAAGGTTTTGCCACTTTCATGGAG
TATTTCTCTTTGGAAAAAATATTCAAAGAGCTTTCTAGTTATGAAGATTTCTTAGATGCT
CGATTTAAAACCATGAAGAAAGATTCCTTAAATTCATCTCATCCAATATCATCATCTGTT
CAGTCTTCAGAACAAATTGAAGAAATGTTTGATTCTCTTTCCTATTTTAAGGGATCTTCT
CTCTTGTTGATGTTGAAAACTTACCTTAGTGAAGATGTGTTTCAACATGCTGTTGTCCTT
TACCTGCATAATCACAGCTATGCATCTATTCAAAGTGATGATCTGTGGGATAGTTTTAAT
GAGGTCACAAACCAAACACTAGATGTAAAGAGAATGATGAAAACCTGGACCCTGCAGAAA
GGATTTCCTTTAGTGACTGTTCAAAAGAAAGGAAAGGAACTTTTTATACAACAAGAGAGA
TTCTTTTTAAATATGAAGCCTGAAATTCAGCCTTCAGATACAAGCTACCTGTGGCATATT
CCACTATCCTATGTCACTGAAGGAAGAAATTATTCAAAATATCAATCGGTATCATTACTG
GATAAGAAATCAGGTGTCATCAATCTTACAGAAGAAGTGCTGTGGGTCAAAGTGAATATA
AACATGAATGGTTATTATATTGTACACTATGCAGATGATGATTGGGAAGCACTAATCCAT
CAGTTGAAAATAAATCCTTATGTTCTGAGTGACAAAGACCGAGCCAACCTTATCAACAAC
ATCTTTGAACTTGCAGGCCTAGGCAAGGTACCTCTCAAGAGGGCCTTTGATTTGATTAAT
TATCTTGGAAATGAGAACCATACTGCACCCATCACCGAAGCCCTGTTTCAGACAGACCTC
ATCTATAACCTCCTTGAAAAACTGGGATACATGGATCTGGCCTCAAGACTGGTGACTAGG
GTATTTAAATTACTTCAAAACCAAATTCAACAACAAACTTGGACTGATGAGGGCACTCCA
TCTATGCGAGAGCTTCGGTCAGCCCTGCTAGAGTTTGCTTGCACCCACAACCTGGGGAAC
TGCTCTACTACTGCCATGAAACTGTTTGATGACTGGATGGCATCCAATGGAACTCAAAGC
CTACCTACTGATGTCATGACAACTGTGTTCAAAGTTGGAGCAAAAACTGACAAAGGCTGG
TAATTCCTTTTGGGCAAATACATTTCTATAGGCTCTGAAGCAGAGAAGAACAAAATACTA
GAAGCACTTGCCAGCTCAGAGGATGTGCGGAAGCTTTACTGGTTAATGAAAAGTAGCCTG
AATGGAGATAACTTCCGAACACAGAAGCTGTCTTTTATCATTAGAACAGTGGGTCGACAT
TTTCCTGGACACTTACTGGCATGGGATTTTGTCAAAGAGAACTGGAATAAGCTTGTACAG
AAGTTCCCTCTGGGGTCCTATACCATACAAAATATTGTTGCTGGATCAACTTACCTGTTT
TCAACAAAGACACATTTATCTGAGGTTCAGGCATTCTTTGAAAATCAGTCAGAGGCAACC
TTCCGGCTTCGTTGTGTCCAGGAGGCTTTGGAAGTCATTCAGTTGAATATCCAGTGGATG
GAGAAGAACCTCAAAAGTCTCACATGGTGGCTG

Protein sequence predicted based on nucleotide sequence

MIENSMFEEEPDVVDLAKEPCLHPLEPDEVEYEPRGSRLLVRGLGEHEMEEDEEDYESSA
KLLGMSFMNRSSGLRNSATGYRQSPDGACSVPSARTMVVCAFVIVVAVSVIMVIYLLPRC
TFTKEGCHKKNQSIGLIQPFATNGKLFPWAQIRLPTAVVPLRYELSLHPNLTSMTFRGSV
TISVQALQVTWNIILHSTGHNISRVTFMSAVSSQEKQAEILEYAYHGQIAIVAPEALLAG
HNYTLKIEYSANISSSYYGFYGFSYTDESNEKKYFAATQFEPLAARSAFPCFDEPAFKAT
FIIKIIRDEQYTALSNMPKKSSVVLDDGLVQDEFSESVKMSTYLVAFIVGEMKNLSQDVN
GTLVSIYAVPEKIGQVHYALETTVKLLEFFQNYFEIQYPLKKLDLVAIPDFEAGAMENWG
LLAFREETLLYDSNTSSMADRKLVTKIIAHELAHQWFGNLVTMKWWNDLWLNEGFATFME
YFSLEKIFKELSSYEDFLDARFKTMKKDSLNSSHPISSSVQSSEQIEEMFDSLSYFKGSS
LLLMLKTYLSEDVFQHAVVLYLHNHSYASIQSDDLWDSFNEVTNQTLDVKRMMKTWTLQK
GFPLVTVQKKGKELFIQQERFFLNMKPEIQPSDTSYLWHIPLSYVTEGRNYSKYQSVSLL
DKKSGVINLTEEVLWVKVNINMNGYYIVHYADDDWEALIHQLKINPYVLSDKDRANLINN
IFELAGLGKVPLKRAFDLINYLGNENHTAPITEALFQTDLIYNLLEKLGYMDLASRLVTR
VFKLLQNQIQQQTWTDEGTPSMRELRSALLEFACTHNLGNCSTTAMKLFDDWMASNGTQS
LPTDVMTTVFKVGAKTDKGW*FLLGKYISIGSEAEKNKILEALASSEDVRKLYWLMKSSL
NGDNFRTQKLSFIIRTVGRHFPGHLLAWDFVKENWNKLVQKFPLGSYTIQNIVAGSTYLF
STKTHLSEVQAFFENQSEATFRLRCVQEALEVIQLNIQWMEKNLKSLTWWL

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
2 errors; 2 non-synonomous errors;
 early stop codon
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable