MEP1A (Homo sapiens) in pLX304

Explanation of Terms

Gene:
Gene Symbol:  MEP1A
Gene Name:  meprin A, alpha (PABA peptide hydrolase)
Original Clone ID:
ccsbBroad304_06576
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00437047
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2239nts
Open reading frame: 1 to 2239

ATGGCTTGGATTAGATCCACTTGCATTCTCTTTTTTACCTTGCTTTTTGCCCACATAGCA
GCTGTACCGATTAAGTATCTTCCTGAAGAAAATGTACATGATGCAGATTTTGGTGAACAG
AAGGATATTTCAGAAATCAATTTAGCTGCAGGCTTGGACCTCTTTCAAGGGGACATCCTC
TTGCAGAAATCCAGAAATGGCCTGAGAGACCCAAACACCAGGTGGACGTTCCCCATTCCT
TACATCTTGGCTGATAATTTGGGGCTGAATGCTAAAGGAGCCATTCTGTATGCCTTTGAG
ATGTTCCGTCTCAAGTCCTGTGTGGATTTCAAGCCCTATGAAGGAGAGAGCTCATATATC
ATATTTCAACAGTTTGATGGGTGCTGGTCTGAGGTTGGTGACCAACATGTGGGACAGAAC
ATTTCCATTGGCCAAGGATGTGCCTATAAGGCCATCATAGAACACGAGATCCTGCATGCT
TTGGGATTTTACCACGAGCAGTCAAGGACGGACCGGGATGATTATGTGAACATCTGGTGG
GACCAAATTCTTTCAGGTTACCAGCACAACTTTGACACCTATGATGATAGCTTAATCACA
GACCTCAATACACCCTATGATTATGAGTCTTTGATGCACTACCAGCCTTTCTCATTTAAC
AAGAATGCAAGTGTTCCCACCATCACAGCCAAGATCCCTGAGTTTAACTCCATTATCGGA
CAACGCCTGGATTTCAGTGCCATTGATTTAGAGAGGCTGAACCGAATGTACAATTGCACC
ACAACTCACACTCTTTTGGACCACTGTACTTTTGAGAAGGCAAACATCTGTGGAATGATT
CAGGGCACCAGAGATGACACTGACTGGGCCCATCAGGACAGTGCTCAGGCTGGAGAAGTG
GATCACACCTTGTTGGGACAATGCACAGGTGCCGGCTACTTCATGCAGTTCAGCACCAGC
TCGGGGTCCGCGGAAGAGGCAGCCCTACTGGAGTCTCGGATTCTTTACCCAAAGAGGAAG
CAGCAGTGCCTGCAATTTTTCTATAAAATGACGGGAAGTCCTTCAGACAGACTCGTTGTC
TGGGTCAGGAGGGATGACAGCACAGGCAATGTTCGCAAGTTGGTGAAGGTGCAGACTTTT
CAAGGAGATGATGACCACAATTGGAAAATTGCCCATGTGGTGCTCAAAGAGGAACAGAAG
TTTCGCTACCTTTTCCAGGGCACAAAAGGCGACCCTCAGAACTCAACTGGGGGAATTTAC
CTAGATGACATCACTCTGACAGAAACCCCCTGCCCCACAGGGGTCTGGACAGTCCGGAAT
TTCTCCCAAGTCCTTGAGAACACCAGCAAAGGGGACAAGCTTCAGAGCCCTCGATTCTAC
AATTCGGAGGGATATGGTTTTGGGTTAACTTTATACCCAAATAGCGGAGAAAGCTCTGGT
TACTTGAGACTTGCTTTTCATGTGTGCAGTGGGGAGAACGATGCTATCCTGGAGTGGCCG
GTAGAAAACAGACAGGTGATAATTACCATCCTTGACCAGGAGCCTGATGTCCGGAACAGG
ATGTCCTCAAGCATGGTGTTCACTACCTCGAAGTCGCACACATCTCCAGCGATAAATGAC
ACTGTCATCTGGGACAGGCCGTCCAGGGTGGGAACCTATCATACAGACTGTAATTGTTTT
AGAAGCATCGACTTGGGCTGGAGTGGTTTCATTTCCCACCAAATGCTGAAAAGGAGGAGT
TTCCTGAAAAATGATGACCTCATCATATTTGTGGACTTTGAAGATATCACCCACCTCAGC
CAGACTGAAGTTCCCACTAAAGGCAAAAGACTGAGCCCCCAAGGCCTCATTCTCCAAGGC
CAGGAGCAGCAGGTCTCCGAAGAAGGTTCGGGAAAGGCCATGTTAGAGGAAGCCCTACCT
GTCAGCCTGAGCCAGGGGCAGCCCAGCCGACAGAAGCGGTCGGTGGAGAACACAGGCCCC
CTGGAGGACCATAACTGGCCACAGTACTTCAGAGACCCATGTGACCCAAACCCTTGCCAA
AATGACGGCATCTGTGTGAACGTGAAGGGGATGGCGAGCTGCAGGTGCATCTCTGGACAT
GCTTTCTTCTACACGGGGGAGCGCTGTCAGGCCGTGCAGGTGCACGGCAGTGTCCTGGGC
ATGGTGATCGGAGGCACGGCTGGCGTGATCTTCTTGACCTTCTCCATCATCGCCATCCTT
TCCCAAAGGCCAAGGAAG

Protein sequence predicted based on nucleotide sequence

MAWIRSTCILFFTLLFAHIAAVPIKYLPEENVHDADFGEQKDISEINLAAGLDLFQGDIL
LQKSRNGLRDPNTRWTFPIPYILADNLGLNAKGAILYAFEMFRLKSCVDFKPYEGESSYI
IFQQFDGCWSEVGDQHVGQNISIGQGCAYKAIIEHEILHALGFYHEQSRTDRDDYVNIWW
DQILSGYQHNFDTYDDSLITDLNTPYDYESLMHYQPFSFNKNASVPTITAKIPEFNSIIG
QRLDFSAIDLERLNRMYNCTTTHTLLDHCTFEKANICGMIQGTRDDTDWAHQDSAQAGEV
DHTLLGQCTGAGYFMQFSTSSGSAEEAALLESRILYPKRKQQCLQFFYKMTGSPSDRLVV
WVRRDDSTGNVRKLVKVQTFQGDDDHNWKIAHVVLKEEQKFRYLFQGTKGDPQNSTGGIY
LDDITLTETPCPTGVWTVRNFSQVLENTSKGDKLQSPRFYNSEGYGFGLTLYPNSGESSG
YLRLAFHVCSGENDAILEWPVENRQVIITILDQEPDVRNRMSSSMVFTTSKSHTSPAIND
TVIWDRPSRVGTYHTDCNCFRSIDLGWSGFISHQMLKRRSFLKNDDLIIFVDFEDITHLS
QTEVPTKGKRLSPQGLILQGQEQQVSEEGSGKAMLEEALPVSLSQGQPSRQKRSVENTGP
LEDHNWPQYFRDPCDPNPCQNDGICVNVKGMASCRCISGHAFFYTGERCQAVQVHGSVLG
MVIGGTAGVIFLTFSIIAILSQRPRK

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable