CENTB1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  ACAP1
Gene Name:  ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
Original Clone ID:
FLH179384.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00043802
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2220nts
Open reading frame: 1 to 2220

ATGACGGTCAAGCTGGATTTCGAGGAGTGTCTCAAGGACTCACCCCGTTTCCGAGCCTCT
ATTGAGCTGGTGGAAGCCGAAGTGTCAGAATTGGAGACCCGTCTGGAAAAGCTCCTGAAA
CTGGGCACTGGTCTCCTGGAAAGTGGGCGCCATTACCTTGCTGCCAGCCGCGCCTTCGTT
GTCGGCATTTGTGACCTGGCCCGCCTGGGTCCACCAGAGCCCATGATGGCGGAGTGTCTG
GAAAAATTCACCGTGAGCCTGAACCACAAGCTGGACAGCCATGCGGAGCTTCTAGATGCC
ACCCAACACACACTGCAGCAGCAGATCCAGACCCTGGTCAAGGAAGGTCTGCGGGGTTTC
CGAGAGGCTCGCCGGGATTTCTGGCGGGGGGCTGAGAGCCTGGAGGCTGCCCTGACCCAC
AACGCAGAGGTTCCCAGGCGCCGGGCCCAGGAGGCAGAAGAGGCAGGAGCTGCTTTGAGG
ACGGCTCGAGCTGGGTACCGGGGACGGGCACTGGATTATGCCCTGCAGATCAACGTGATT
GAGGACAAGAGGAAGTTTGACATCATGGAGTTTGTGCTGCGTTTGGTGGAGGCCCAGGCT
ACCCATTTCCAGCAGGGCCATGAGGAGCTGAGCCGGCTGTCCCAGTATCGAAAGGAGCTG
GGCGCCCAGTTGCACCAGCTGGTCTTGAATTCAGCACGAGAGAAGAGGGACATGGAGCAG
AGACACGTGCTGCTGAAACAGAAGGAGCTGGGTGGGGAGGAGCCAGAACCAAGCTTAAGA
GAGGGGCCTGGTGGCCTGGTGATGGAAGGACATCTCTTCAAACGGGCCAGCAACGCATTT
AAGACCTGGAGCAGACGCTGGTTCACCATTCAGAGCAACCAACTGGTTTACCAGAAGAAG
TACAAGGACCCTGTGACTGTGGTGGTGGATGACCTTCGTCTCTGCACAGTGAAACTCTGC
CCTGACTCAGAAAGGCGGTTCTGCTTTGAGGTGGTGTCCACCAGCAAGTCCTGCCTCCTC
CAGGCTGACTCAGAGCGCCTCCTGCAGCTGTGGGTCAGTGCTGTGCAGAGCAGCATTGCT
TCTGCCTTCAGTCAGGCTCGCCTTGATGACAGCCCCCGGGGTCCAGGCCAGGGCTCAGGA
CACCTGGCCATAGGCTCTGCTGCCACCCTGGGCTCTGGTGGAATGGCCAGGGGAAGGGAG
CCTGGGGGAGTCGGGCACGTGGTGGCCCAGGTCCAGAGTGTGGATGGCAATGCCCAGTGC
TGCGACTGCCGGGAGCCAGCCCCGGAGTGGGCCAGCATCAACCTTGGTGTCACCCTCTGC
ATTCAGTGTTCCGGCATCCACAGGAGCCTTGGTGTTCACTTCTCCAAAGTCCGGTCTCTG
ACCCTTGACTCATGGGAGCCAGAACTAGTGAAGCTCATGTGTGAGCTGGGAAATGTCATC
ATCAACCAGATCTATGAGGCCCGCGTGGAGGCCATGGCAGTGAAGAAACCAGGGCCCAGC
TGCTCCCGGCAGGAGAAGGAGGCCTGGATTCACGCTAAATACGTGGAGAAGAAGTTCCTG
ACCAAGCTGCCTGAGATTCGAGGGCGAAGAGGTGGCCGGGGGCGCCCAAGGGGGCAGCCT
CCTGTGCCCCCAAAGCCTTCCATCAGGCCCCGGCCAGGGAGCTTGAGATCCAAGCCAGAG
CCCCCCTCTGAGGACCTGGGAAGCCTGCACCCTGGGGCCCTACTGTTTCGAGCGTCTGGG
CATCCTCCATCTCTTCCCACCATGGCTGATGCCCTTGCCCATGGAGCTGATGTCAACTGG
GTCAATGGGGGCCAAGATAATGCCACACCGCTGATCCAGGCCACAGCTGCTAATTCTCTT
CTGGCCTGTGAGTTTCTCCTCCAGAACGGGGCGAACGTGAACCAAGCGGACAGTGCGGGC
CGGGGCCCGCTGCACCACGCAACCATTCTTGGCCACACGGGGCTCGCCTGCCTGTTCCTG
AAACGGGGAGCTGATCTGGGGGCTCGAGACTCTGAAGGCAGGGACCCTCTGACCATCGCC
ATGGAAACAGCCAACGCTGACATCGTCACCCTGCTACGACTGGCAAAGATGAGGGAGGCT
GAAGCGGCCCAGGGGCAGGCAGGAGATGAGACGTATCTTGACATCTTCCGCGACTTCTCC
CTCATGGCGTCAGACGACCCGGAGAAGCTGAGCCGTCGCAGTCATGACCTCCACACGCTG

Protein sequence predicted based on nucleotide sequence

MTVKLDFEECLKDSPRFRASIELVEAEVSELETRLEKLLKLGTGLLESGRHYLAASRAFV
VGICDLARLGPPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGF
REARRDFWRGAESLEAALTHNAEVPRRRAQEAEEAGAALRTARAGYRGRALDYALQINVI
EDKRKFDIMEFVLRLVEAQATHFQQGHEELSRLSQYRKELGAQLHQLVLNSAREKRDMEQ
RHVLLKQKELGGEEPEPSLREGPGGLVMEGHLFKRASNAFKTWSRRWFTIQSNQLVYQKK
YKDPVTVVVDDLRLCTVKLCPDSERRFCFEVVSTSKSCLLQADSERLLQLWVSAVQSSIA
SAFSQARLDDSPRGPGQGSGHLAIGSAATLGSGGMARGREPGGVGHVVAQVQSVDGNAQC
CDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVRSLTLDSWEPELVKLMCELGNVI
INQIYEARVEAMAVKKPGPSCSRQEKEAWIHAKYVEKKFLTKLPEIRGRRGGRGRPRGQP
PVPPKPSIRPRPGSLRSKPEPPSEDLGSLHPGALLFRASGHPPSLPTMADALAHGADVNW
VNGGQDNATPLIQATAANSLLACEFLLQNGANVNQADSAGRGPLHHATILGHTGLACLFL
KRGADLGARDSEGRDPLTIAMETANADIVTLLRLAKMREAEAAQGQAGDETYLDIFRDFS
LMASDDPEKLSRRSHDLHTL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 182
End on reference sequence 2404
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable