RASGEF1A (Homo sapiens) in pLX304

Explanation of Terms

Gene:
Gene Symbol:  RASGEF1A
Gene Name:  RasGEF domain family, member 1A
Original Clone ID:
ccsbBroad304_13418
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00438176
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1285nts
Open reading frame: 1 to 1285

ATGGAGCACCTTGTTCCCACGGTGGACTATTACCCCGATAGGACGTACATCTTCACCTTT
CTCCTGAGCTCCCGGGTCTTTATGCCCCCTCATGACCTGCTGGCCCGCGTGGGGCAGATC
TGCGTGGAGCAGAAGCAGCAGCTGGAAGCCGGGCCTGAAAAGGCCAAGCTGAAGTCTTTC
TCAGCCAAGATCGTGCAGCTCCTGAAGGAGTGGACCGAGGCCTTCCCCTATGACTTCCAG
GATGAGAAGGCCATGGCCGAGCTGAAAGCCATCACACACCGTGTCACCCAGTGTGATGAG
GAGAATGGCACAGTGAAGAAGGCCATTGCCCAGATGACACAGAGCCTGTTGCTGTCCTTG
GCTGCCCGGAGCCAGCTCCAGGAACTGCGAGAGAAGCTCCGGCCACCGGCTGTAGACAAG
GGGCCCATCCTCAAGACCAAGCCACCAGCCGCCCAGAAGGACATCCTGGGCGTGTGCTGC
GACCCCCTGGTGCTGGCCCAGCAGCTGACTCACATTGAGCTGGACAGGGTCAGCAGCATT
TACCCTGAGGACTTGATGCAGATCGTCAGCCACATGGACTCATTGGACAACCACAGGTGC
CGAGGGGACCTGACCAAGACCTACAGCCTGGAGGCCTATGACAACTGGTTCAACTGCCTG
AGCATGCTGGTGGCCACTGAGGTGTGCCGGGTGGTGAAGAAGAAACACCGGACCCGCATG
TTGGAGTTCTTCATTGATGTGGCCCGGGAGTGCTTCAACATCGGGAACTTCAACTCCATG
ATGGCCATCATCTCTGGCATGAACCTCAGTCCTGTGGCAAGGCTGAAGAAAACTTGGTCC
AAGGTCAAGACAGCCAAGTTTGATGTCTTGGAGCATCACATGGACCCGTCCAGCAACTTC
TGCAACTACCGTACAGCCCTGCAGGGGGCCACGCAGAGGTCCCAGATGGCCAACAGCAGC
CGTGAAAAGATCGTCATCCCTGTGTTCAACCTCTTCGTTAAGGACATCTACTTCCTGCAC
AAAATCCATACCAACCACCTGCCCAACGGGCACATTAACTTTAAGAAATTCTGGGAGATC
TCCAGACAGATCCATGAGTTCATGACATGGACACAGGTAGAGTGTCCTTTCGAGAAGGAC
AAGAAGATTCAGAGTTACCTGCTCACGGCGCCCATCTACAGCGAGGAAGCTCTCTTCGTC
GCCTCCTTTGAAAGTGAGGGTCCCGAGAACCACGTGGAAAAAGACAGCTGGAAGACCCTC
AGGACCACCCTTCTGAACAGAGCC

Protein sequence predicted based on nucleotide sequence

MEHLVPTVDYYPDRTYIFTFLLSSRVFMPPHDLLARVGQICVEQKQQLEAGPEKAKLKSF
SAKIVQLLKEWTEAFPYDFQDEKAMAELKAITHRVTQCDEENGTVKKAIAQMTQSLLLSL
AARSQLQELREKLRPPAVDKGPILKTKPPAAQKDILGVCCDPLVLAQQLTHIELDRVSSI
YPEDLMQIVSHMDSLDNHRCRGDLTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRM
LEFFIDVARECFNIGNFNSMMAIISGMNLSPVARLKKTWSKVKTAKFDVLEHHMDPSSNF
CNYRTALQGATQRSQMANSSREKIVIPVFNLFVKDIYFLHKIHTNHLPNGHINFKKFWEI
SRQIHEFMTWTQVECPFEKDKKIQSYLLTAPIYSEEALFVASFESEGPENHVEKDSWKTL
RTTLLNRA

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable