KDM3A (Homo sapiens) in pLX304

Explanation of Terms

Gene:
Gene Symbol:  KDM3A
Gene Name:  lysine (K)-specific demethylase 3A
Original Clone ID:
ccsbBroad304_14209
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00438818
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3577nts
Open reading frame: 1 to 3577

ATGGTGCTCACGCTCGGAGAAAGTTGGCCGGTATTGGTGGGGAGGAGGTTTCTCAGTCTG
TCCGCAGCCGACGGCAGCGATGGCAGCCACGACAGCTGGGACGTGGAGCGCGTCGCCGAG
TGGCCCTGGCTCTCCGGGACCATTCGAGCTGTTTCCCACACCGACGTTACCAAGAAGGAT
CTGAAGGTGTGTGTGGAATTTGATGGGGAATCTTGGAGGAAAAGAAGATGGATAGAAGTC
TACAGCCTTCTAAGGAGAGCATTTTTAGTAGAACATAATTTGGTTTTAGCTGAACGAAAG
TCACCTGAAATTTCTGAACGAATTGTACAGTGGCCTGCAATAACGTACAAACCTCTGTTG
GACAAAGCTGGTTTGGGATCCATAACTTCTGTTCGCTTTCTGGGAGATCAACAAAGAGTA
TTTCTTTCTAAAGACCTTTTGAAGCCTATACAGGATGTAAACAGTCTTCGACTTTCTCTT
ACGGATAATCAGATTGTCAGTAAAGAATTTCAAGCTTTGATTGTGAAGCATTTAGATGAA
AGCCATCTTTTAAAAGGTGACAAAAACTTAGTTGGTTCAGAAGTAAAAATTTATAGCTTG
GACCCATCTACTCAGTGGTTTTCAGCAACCGTTGTAAATGGAAACCCAGCATCAAAAACT
CTTCAAGTCAACTGTGAGGAGATTCCAGCACTGAAAATTGTTGATCCGTCACTGATTCAT
GTTGAAGTTGTACACGATAACCTTGTGACATGTGGTAATTCTGCAAGAATTGGAGCTGTA
AAACGCAAGTCTTCTGAGAATAATGGAACCCTGGTTTCCAAACAAGCAAAATCTTGCTCT
GAGGCCTCTCCCAGTATGTGTCCTGTGCAGTCTGTACCTACAACAGTTTTTAAGGAGATA
CTGCTTGGCTGTACTGCAGCAACTCCACCTAGTAAGGACCCAAGACAGCAAAGTACTCCC
CAGGCTGCCAACTCTCCACCTAACCTTGGAGCAAAAATTCCTCAAGGATGTCATAAACAA
AGTTTACCAGAGGAAATTTCTTCCTGTCTAAATACAAAGTCTGAAGCTCTGAGAACAAAA
CCAGATGTCTGCAAAGCAGGGTTGCTCTCAAAGTCCTCTCAGATTGGAACTGGAGACTTG
AAAATTCTGACTGAGCCAAAAGGCAGCTGTACTCAGCCTAAGACAAACACTGATCAGGAA
AACAGATTGGAGTCTGTTCCACAAGCATTGACTGGCCTTCCTAAGGAGTGCTTACCTACA
AAGGCTTCTTCTAAGGCAGAATTGGAAATTGCCAATCCTCCTGAACTGCAGAAGCACCTA
GAACATGCACCTTCCCCATCGGATGTTTCAAATGCACCAGAAGTGAAAGCAGGTGTCAAT
AGTGATAGCCCTAATAACTGTTCAGGAAAAAAGGTAGAACCTTCAGCTTTAGCTTGCCGA
TCACAGAATTTAAAGGAATCTTCAGTAAAAGTAGATAATGAAAGCTGTTGTTCAAGAAGC
AACAATAAAATCCAGAATGCCCCATCCAGGAAGTCGGTTTTGACAGACCCAGCTAAACTC
AAAAAGCTGCAACAGAGTGGCGAGGCCTTCGTACAGGATGATTCTTGTGTGAACATCGTG
GCACAGTTGCCTAAATGCCGAGAGTGTCGCTTGGACAGTCTCCGCAAGGATAAGGAGCAA
CAGAAGGACTCACCTGTGTTTTGCCGCTTCTTTCACTTCAGGAGGTTACAATTCAACAAA
CATGGTGTGTTGCGGGTAGAAGGCTTCTTAACACCAAACAAGTATGACAATGAAGCAATT
GGCTTGTGGTTACCTTTAACCAAAAACGTTGTGGGGATTGATTTGGACACAGCAAAGTAC
ATCTTGGCCAACATTGGAGACCACTTCTGTCAAATGGTGATTTCTGAAAAGGAAGCTATG
TCAACTATTGAGCCACACAGACAGGTTGCTTGGAAGCGAGCTGTCAAAGGTGTTCGAGAA
ATGTGTGATGTGTGCGACACCACCATCTTCAACCTGCACTGGGTGTGTCCTCGGTGTGGG
TTTGGAGTATGTGTGGACTGCTACCGGATGAAGAGAAAGAATTGCCAACAGGGTGCTGCT
TACAAGACTTTCTCTTGGCTAAAATGTGTGAAGAGTCAGATACATGAACCAGAGAACTTA
ATGCCCACACAGATCATTCCTGGAAAAGCACTCTATGATGTTGGAGACATTGTTCATTCT
GTAAGAGCGAAATGGGGAATAAAGGCAAACTGCCCTTGTTCAAACAGGCAATTCAAACTC
TTTTCAAAGCCAGCCTCAAAGGAAGACCTAAAACAGACTTCTTTAGCTGGAGAAAAACCG
ACTCTTGGTGCAGTGCTCCAGCAGAATCCCTCAGTGTTGGAGCCAGCAGCTGTGGGTGGG
GAAGCAGCCTCCAAGCCAGCCGGCAGCATGAAGCCTGCCTGTCCAGCCAGCACATCTCCT
CTAAACTGGCTGGCCGACCTAACCAGCGGGAATGTCAACAAGGAAAACAAGGAAAAACAA
CCAACAATGCCAATTTTAAAGAATGAAATCAAATGCCTTCCACCCCTCCCACCTTTAAGC
AAATCCAGCACAGTCCTCCATACGTTTAACAGCACAATTTTGACACCCGTAAGCAACAAC
AATTCTGGTTTCCTCCGGAATCTCTTGAATTCTTCTACAGGAAAGACAGAAAATGGACTC
AAGAATACACCAAAAATCCTTGATGACATCTTTGCCTCTTTGGTGCAAAATAAGACGACT
TCTGATTTATCTAAGAGGCCTCAAGGACTAACCATCAAGCCCAGCATTCTGGGCTTTGAC
ACTCCTCACTATTGGCTTTGTGATAATCGCTTGCTGTGCTTGCAAGACCCCAACAATAAG
AGCAACTGGAATGTGTTTAGGGAGTGCTGGAAACAAGGGCAGCCAGTGATGGTGTCTGGA
GTGCATCATAAATTGAACTCTGAACTTTGGAAACCTGAATCCTTCAGGAAAGAGTTTGGT
GAGCAGGAAGTAGACCTAGTTAATTGTAGGACCAATGAAATCATCACAGGAGCCACAGTA
GGAGACTTCTGGGATGGATTTGAAGATGTTCCAAATCGTTTGAAAAATGAAAAAGAACCA
ATGGTGTTGAAACTTAAGGACTGGCCACCAGGAGAAGATTTTAGAGATATGATGCCTTCC
AGGTTTGATGATCTGATGGCCAACATTCCACTGCCCGAGTACACAAGGCGAGATGGCAAA
CTGAATTTGGCCTCTAGGCTGCCAAACTACTTTGTTCGGCCAGATCTGGGCCCCAAGATG
TATAATGCTTATGGATTAATCACTCCTGAAGATCGGAAATATGGAACAACAAATCTTCAC
TTAGATGTATCTGATGCAGCTAATGTCATGGTCTATGTGGGAATTCCCAAAGGACAGTGT
GAGCAAGAAGAAGAAGTCCTTAAGACCATCCAAGATGGAGATTCTGACGAACTCACAATA
AAGCGATTTATTGAAGGAAAAGAGAAGCCAGGAGCACTGTGGCACATATATGCTGCAAAG
GACACGGAGAAGATAAGGGAATTTCTTAAAAAGATA

Protein sequence predicted based on nucleotide sequence

MVLTLGESWPVLVGRRFLSLSAADGSDGSHDSWDVERVAEWPWLSGTIRAVSHTDVTKKD
LKVCVEFDGESWRKRRWIEVYSLLRRAFLVEHNLVLAERKSPEISERIVQWPAITYKPLL
DKAGLGSITSVRFLGDQQRVFLSKDLLKPIQDVNSLRLSLTDNQIVSKEFQALIVKHLDE
SHLLKGDKNLVGSEVKIYSLDPSTQWFSATVVNGNPASKTLQVNCEEIPALKIVDPSLIH
VEVVHDNLVTCGNSARIGAVKRKSSENNGTLVSKQAKSCSEASPSMCPVQSVPTTVFKEI
LLGCTAATPPSKDPRQQSTPQAANSPPNLGAKIPQGCHKQSLPEEISSCLNTKSEALRTK
PDVCKAGLLSKSSQIGTGDLKILTEPKGSCTQPKTNTDQENRLESVPQALTGLPKECLPT
KASSKAELEIANPPELQKHLEHAPSPSDVSNAPEVKAGVNSDSPNNCSGKKVEPSALACR
SQNLKESSVKVDNESCCSRSNNKIQNAPSRKSVLTDPAKLKKLQQSGEAFVQDDSCVNIV
AQLPKCRECRLDSLRKDKEQQKDSPVFCRFFHFRRLQFNKHGVLRVEGFLTPNKYDNEAI
GLWLPLTKNVVGIDLDTAKYILANIGDHFCQMVISEKEAMSTIEPHRQVAWKRAVKGVRE
MCDVCDTTIFNLHWVCPRCGFGVCVDCYRMKRKNCQQGAAYKTFSWLKCVKSQIHEPENL
MPTQIIPGKALYDVGDIVHSVRAKWGIKANCPCSNRQFKLFSKPASKEDLKQTSLAGEKP
TLGAVLQQNPSVLEPAAVGGEAASKPAGSMKPACPASTSPLNWLADLTSGNVNKENKEKQ
PTMPILKNEIKCLPPLPPLSKSSTVLHTFNSTILTPVSNNNSGFLRNLLNSSTGKTENGL
KNTPKILDDIFASLVQNKTTSDLSKRPQGLTIKPSILGFDTPHYWLCDNRLLCLQDPNNK
SNWNVFRECWKQGQPVMVSGVHHKLNSELWKPESFRKEFGEQEVDLVNCRTNEIITGATV
GDFWDGFEDVPNRLKNEKEPMVLKLKDWPPGEDFRDMMPSRFDDLMANIPLPEYTRRDGK
LNLASRLPNYFVRPDLGPKMYNAYGLITPEDRKYGTTNLHLDVSDAANVMVYVGIPKGQC
EQEEEVLKTIQDGDSDELTIKRFIEGKEKPGALWHIYAAKDTEKIREFLKKI

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
1 error; 1 non-synonomous errors
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable