ANKRD27 (Homo sapiens) in pLX304

Explanation of Terms

Gene:
Gene Symbol:  ANKRD27
Gene Name:  ankyrin repeat domain 27 (VPS9 domain)
Original Clone ID:
ccsbBroad304_04327
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00439852
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3151nts
Open reading frame: 1 to 3151

ATGGCTCTGTATGATGAAGACCTCCTGAAAAATCCTTTCTATCTGGCTCTGCAAAAGTGC
CGCCCTGACTTGTGCAGCAAAGTGGCCCAAATCCATGGCATTGTCTTAGTACCCTGCAAA
GGAAGCCTGTCGAGCAGCATCCAGTCTACTTGTCAGTTTGAGTCCTACATTTTGATACCT
GTGGAAGAGCATTTTCAGACCTTAAATGGAAAGGATGTCTTTATTCAAGGGAACAGGATT
AAATTAGGAGCTGGTTTTGCCTGTCTTCTCTCAGTGCCCATTCTCTTTGAAGAAACTTTC
TACAATGAAAAAGAAGAGAGTTTCAGCATCCTGTGTATAGCCCATCCTTTGGAAAAGAGA
GAGAGTTCAGAAGAGCCTTTGGCACCCTCAGATCCCTTTTCCCTGAAAACCATTGAAGAT
GTGAGAGAGTTCTTGGGAAGACACTCCGAGCGATTTGACAGGAACATCGCCTCTTTCCAT
CGAACATTCCGAGAATGCGAGAGAAAGAGCCTCCGTCACCACATAGACTCAGCGAATGCT
CTCTACACCAAATGCCTCCAGCAGCTTCTGAGGGACTCTCACCTGAAAATGCTCGCCAAG
CAGGAGGCCCAGATGAACCTGATGAAGCAGGCAGTGGAGATATACGTCCATCATGAAATT
TACAACCTGATCTTTAAATACGTGGGGACCATGGAGGCAAGTGAGGATGCGGCCTTTAAC
AAAATCACAAGAAGCCTTCAAGATCTTCAGCAGAAAGATATTGGTGTGAAACCGGAGTTC
AGCTTTAACATACCTCGTGCCAAAAGAGAGCTGGCTCAGCTGAACAAATGCACCTCCCCA
CAGCAGAAGCTTGTCTGCTTGCGAAAAGTGGTGCAGCTCATTACACAGTCTCCAAGCCAG
AGAGTGAACCTGGAGACCATGTGTGCTGATGATCTGCTATCAGTCCTGTTATACTTGCTT
GTGAAAACGGAGATCCCTAATTGGATGGCAAATTTGAGTTACATCAAAAACTTCAGGTTT
AGCAGCTTGGCAAAGGATGAACTGGGATACTGCCTGACCTCATTCGAAGCTGCCATTGAA
TATATTCGGCAAGGAAGCCTCTCTGCTAAACCCCCTGAGTCTGAGGGATTTGGAGACAGG
CTGTTCCTTAAGCAGAGAATGAGCTTACTCTCTCAGATGACTTCGTCTCCCACCGACTGC
CTGTTTAAGCACATTGCATCAGGTAACCAGAAAGAAGTGGAGAGACTTCTGAGCCAAGAG
GACCATGATAAAGATACCGTCCAAAAGATGTGTCACCCTCTCTGCTTCTGCGATGACTGT
GAGAAACTCGTCTCTGGGAGGTTGAATGATCCCTCAGTTGTCACTCCATTCTCCAGAGAC
GACAGGGGGCACACCCCTCTCCATGTGGCTGCTGTCTGTGGGCAGGCATCCCTCATCGAC
CTCCTGGTTTCCAAGGGCGCCATGGTAAATGCCACAGACTACCATGGAGCCACTCCGCTC
CACCTGGCCTGTCAGAAGGGCTACCAGAGCGTGACGCTGCTGCTGCTGCACTACAAGGCC
AGCGCGGAAGTGCAGGACAACAATGGGAATACGCCACTCCACCTGGCCTGCACCTACGGC
CACGAGGACTGTGTGAAGGCTCTGGTTTACTACGACGTGGAGTCGTGCAGACTTGACATT
GGCAATGAGAAAGGAGACACCCCTCTACACATTGCTGCCCGCTGGGGCTACCAAGGCGTC
ATAGAGACATTGCTGCAGAACGGAGCGTCCACCGAGATCCAGAACAGACTGAAGGAGACG
CCCCTCAAGTGTGCATTAAACTCAAAGATTCTGTCTGTAATGGAAGCCTATCACCTGTCC
TTCGAGAGGAGGCAGAAGTCGTCCGAGGCCCCTGTGCAGTCCCCGCAGCGCTCCGTGGAC
TCCATCAGCCAAGAGTCCTCCACTTCCAGCTTCTCCTCCATGTCAGCCAGCTCAAGGCAG
GAGGAGACCAAGAAGGACTACAGAGAGGTAGAAAAACTTTTGAGAGCAGTTGCTGATGGA
GATCTAGAAATGGTGCGTTACCTGTTGGAATGGACAGAGGAGGACCTGGAGGATGCGGAG
GACACTGTCAGTGCAGCGGACCCCGAATTCTGTCACCCGTTGTGCCAGTGCCCCAAGTGT
GCCCCAGCTCAGAAGAGGCTGGCGAAGGTTCCTGCCAGTGGGCTTGGTGTGAACGTGACC
AGCCAGGACGGCTCCTCCCCGCTGCATGTCGCCGCCCTGCACGGCCGGGCGGACCTCATC
CCCCTCCTGCTGAAGCACGGGGCCAACGCAGGTGCCAGGAACGCAGACCAAGCCGTCCCG
CTCCACCTGGCCTGCCAGCAGGGCCACTTTCAGGTGGTGAAGTGTCTGTTAGATTCGAAT
GCAAAACCCAATAAGAAGGACCTCAGTGGAAACACGCCCCTCATTTACGCCTGCTCCGGT
GGCCATCACGAGCTTGTGGCACTGCTGCTACAGCACGGGGCCTCCATTAACGCTTCTAAC
AATAAGGGCAACACAGCGCTGCACGAGGCTGTGATTGAAAAGCACGTCTTCGTGGTAGAG
CTGCTTCTGCTCCACGGAGCGTCAGTTCAGGTGCTGAACAAGCGGCAGCGCACGGCTGTA
GACTGTGCTGAACAGAATTCAAAAATAATGGAATTGCTTCAGGTGGTACCAAGCTGTGTT
GCTTCATTAGATGATGTGGCTGAAACTGACCGCAAGGAGTATGTCACTGTTAAGATCAGG
AAAAAATGGAACTCAAAACTGTATGATCTACCAGATGAGCCTTTTACAAGACAGTTTTAC
TTTGTCCACTCAGCTGGTCAGTTTAAGGGAAAGACTTCAAGGGAGATTATGGCAAGAGAT
AGAAGTGTCCCTAATTTAACCGAAGGTTCTTTGCATGAGCCAGGGAGGCAAAGTGTCACA
CTGAGACAGAATAACCTGCCAGCTCAGAGTGGATCTCATGCTGCTGAGAAAGGCAACAGC
GACTGGCCAGAGAGGCCTGGACTGACACAGACTGGCCCTGGACACAGACGGATGCTGCGG
AGACACACGGTAGAGGATGCGGTCGTGTCCCAGGGCCCGGAGGCTGCTGGCCCCCTCTCC
ACTCCCCAAGAGGTTAGTGCTTCCCGGTCC

Protein sequence predicted based on nucleotide sequence

MALYDEDLLKNPFYLALQKCRPDLCSKVAQIHGIVLVPCKGSLSSSIQSTCQFESYILIP
VEEHFQTLNGKDVFIQGNRIKLGAGFACLLSVPILFEETFYNEKEESFSILCIAHPLEKR
ESSEEPLAPSDPFSLKTIEDVREFLGRHSERFDRNIASFHRTFRECERKSLRHHIDSANA
LYTKCLQQLLRDSHLKMLAKQEAQMNLMKQAVEIYVHHEIYNLIFKYVGTMEASEDAAFN
KITRSLQDLQQKDIGVKPEFSFNIPRAKRELAQLNKCTSPQQKLVCLRKVVQLITQSPSQ
RVNLETMCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFSSLAKDELGYCLTSFEAAIE
YIRQGSLSAKPPESEGFGDRLFLKQRMSLLSQMTSSPTDCLFKHIASGNQKEVERLLSQE
DHDKDTVQKMCHPLCFCDDCEKLVSGRLNDPSVVTPFSRDDRGHTPLHVAAVCGQASLID
LLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYKASAEVQDNNGNTPLHLACTYG
HEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGYQGVIETLLQNGASTEIQNRLKET
PLKCALNSKILSVMEAYHLSFERRQKSSEAPVQSPQRSVDSISQESSTSSFSSMSASSRQ
EETKKDYREVEKLLRAVADGDLEMVRYLLEWTEEDLEDAEDTVSAADPEFCHPLCQCPKC
APAQKRLAKVPASGLGVNVTSQDGSSPLHVAALHGRADLIPLLLKHGANAGARNADQAVP
LHLACQQGHFQVVKCLLDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASN
NKGNTALHEAVIEKHVFVVELLLLHGASVQVLNKRQRTAVDCAEQNSKIMELLQVVPSCV
ASLDDVAETDRKEYVTVKIRKKWNSKLYDLPDEPFTRQFYFVHSAGQFKGKTSREIMARD
RSVPNLTEGSLHEPGRQSVTLRQNNLPAQSGSHAAEKGNSDWPERPGLTQTGPGHRRMLR
RHTVEDAVVSQGPEAAGPLSTPQEVSASRS

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable