PCOLCE (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  PCOLCE
Gene Name:  procollagen C-endopeptidase enhancer
Original Clone ID:
FLH181453.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
HsCD00043996
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1350nts
Open reading frame: 1 to 1350

ATGCTGCCTGCAGCCACAGCCTCCCTCCTGGGGCCCCTCCTCACTGCCTGCGCCCTGCTG
CCTTTTGCCCAGGGCCAGACCCCCAACTACACCAGACCCGTGTTCCTGTGCGGAGGGGAT
GTGAAGGGGGAATCAGGTTACGTGGCAAGTGAGGGGTTCCCCAACCTCTACCCCCCTAAT
AAGGAGTGCATCTGGACCATAACGGTCCCCGAGGGCCAGACTGTGTCCCTCTCATTCCGA
GTCTTCGACCTGGAGCTGCACCCCGCCTGCCGCTACGATGCTCTGGAGGTCTTCGCTGGG
TCTGGGACTTCCGGCCAGCGGCTCGGACGCTTTTGTGGGACCTTCCGGCCTGCGCCCCTA
GTCGCCCCCGGCAACCAGGTGACCCTGAGGATGACGACGGATGAGGGCACAGGAGGACGA
GGCTTCCTGCTCTGGTACAGCGGGCGGGCCACCTCGGGCACTGAGCACCAATTTTGCGGG
GGGCGGCTGGAGAAGGCCCAGGGAACCCTGACCACGCCCAACTGGCCCGAGTCCGATTAC
CCCCCGGGCATCAGCTGTTCCTGGCACATCATCGCGCCCCCGGACCAGGTCATCGCGCTG
ACCTTCGAGAAGTTTGACCTGGAGCCGGACACCTACTGCCGCTATGACTCGGTCAGCGTG
TTCAACGGAGCCGTGAGCGACGACTCCCGGAGGCTGGGGAAGTTCTGCGGCGACGCAGTC
CCGGGCTCCATCTCCTCCGAAGGGAATGAACTCCTCGTCCAGTTCGTCTCAGATCTCAGT
GTCACCGCTGATGGCTTCTCAGCCTCCTACAAGACCCTGCCGCGGGGCACTGCCAAAGAA
GGGCAAGGGCCCGGCCCCAAACGGGGAACTGAGCCTAAAGTCAAGCTGCCCCCCAAGTCC
CAACCTCCGGAGAAAACAGAGGAATCTCCTTCAGCCCCTGATGCACCCACCTGCCCAAAG
CAGTGCCGCCGGACAGGCACCTTGCAGAGCAACTTCTGTGCCAGCAGCCTTGTGGTGACT
GCGACAGTGAAGTCCATGGTTCGGGAGCCAGGGGAGGGCCTTGCCGTGACTGTCAGTCTT
ATTGGTGCTTATAAAACTGGAGGACTGGACCTGCCTTCTCCACCCACTGGTGCCTCCCTG
AAGTTTTACGTGCCTTGCAAGCAGTGCCCCCCCATGAAGAAAGGAGTCAGTTATCTGCTG
ATGGGCCAGGTAGAAGAGAACAGAGGCCCCGTCCTTCCTCCAGAGAGCTTTGTGGTTCTC
CACCGGCCCAACCAGGACCAGATCCTCACCAACCTAAGCAAGAGGAAGTGCCCCTCTCAA
CCTGTGCGGGCTGCTGCATCCCAGGACTAG

Protein sequence predicted based on nucleotide sequence

MLPAATASLLGPLLTACALLPFAQGQTPNYTRPVFLCGGDVKGESGYVASEGFPNLYPPN
KECIWTITVPEGQTVSLSFRVFDLELHPACRYDALEVFAGSGTSGQRLGRFCGTFRPAPL
VAPGNQVTLRMTTDEGTGGRGFLLWYSGRATSGTEHQFCGGRLEKAQGTLTTPNWPESDY
PPGISCSWHIIAPPDQVIALTFEKFDLEPDTYCRYDSVSVFNGAVSDDSRRLGKFCGDAV
PGSISSEGNELLVQFVSDLSVTADGFSASYKTLPRGTAKEGQGPGPKRGTEPKVKLPPKS
QPPEKTEESPSAPDAPTCPKQCRRTGTLQSNFCASSLVVTATVKSMVREPGEGLAVTVSL
IGAYKTGGLDLPSPPTGASLKFYVPCKQCPPMKKGVSYLLMGQVEENRGPVLPPESFVVL
HRPNQDQILTNLSKRKCPSQPVRAAASQD*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 101
End on reference sequence 1450
Mutations:
None
Discrepancy:
g1338a
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable