MCM2 (Homo sapiens) in pLX304

Explanation of Terms

Gene:
Gene Symbol:  MCM2
Gene Name:  minichromosome maintenance complex component 2
Original Clone ID:
ccsbBroad304_00986
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00439986
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2713nts
Open reading frame: 1 to 2713

ATGGCGGAATCATCGGAATCCTTCACCATGGCATCCAGCCCGGCCCAGCGTCGGCGAGGC
AATGATCCTCTCACCTCCAGCCCTGGCCGAAGCTCCCGGCGTACTGATGCCCTCACCTCC
AGCCCTGGCCGTGACCTTCCACCATTTGAGGATGAGTCCGAGGGGCTCCTAGGCACAGAG
GGGCCCCTGGAGGAAGAAGAGGATGGAGAGGAGCTCATTGGAGATGGCATGGAAAGGGAC
TACCGCGCCATCCCAGAGCTGGACGCCTATGAGGCCGAGGGACTGGCTCTGGATGATGAG
GACGTAGAGGAGCTGACGGCCAGTCAGAGGGAGGCAGCAGAGCGGGCCATGCGGCAGCGT
GACCGGGAGGCTGGCCGGGGCCTGGGCCGCATGCGCCGTGGGCTCCTGTATGACAGCGAT
GAGGAGGACGAGGAGCGCCCTGCCCGCAAGCGCCGCCAGGTGGAGCGGGCCACGGAGGAC
GGCGAGGAGGACGAGGAGATGATCGAGAGCATCGAGAACCTGGAGGATCTCAAAGGCCAC
TCTGTGCGCGAGTGGGTGAGCATGGCGGGCCCCCGGCTGGAGATCCACCACCGCTTCAAG
AACTTCCTGCGCACTCACGTCGACAGCCACGGCCACAACGTCTTCAAGGAGCGCATCAGC
GACATGTGCAAAGAGAACCGTGAGAGCCTGGTGGTGAACTATGAGGACTTGGCAGCCAGG
GAGCACGTGCTGGCCTACTTCCTGCCTGAGGCACCGGCGGAGCTGCTGCAGATCTTTGAT
GAGGCTGCCCTGGAGGTGGTACTGGCCATGTACCCCAAGTACGACCGCATCACCAACCAC
ATCCATGTCCGCATCTCCCACCTGCCTCTGGTGGAGGAGCTGCGCTCGCTGAGGCAGCTG
CATCTGAACCAGCTGATCCGCACCAGTGGGGTGGTGACCAGCTGCACTGGCGTCCTGCCC
CAGCTCAGCATGGTCAAGTACAACTGCAACAAGTGCAATTTCGTCCTGGGTCCTTTCTGC
CAGTCCCAGAACCAGGAGGTGAAACCAGGCTCCTGTCCTGAGTGCCAGTCGGCCGGCCCC
TTTGAGGTCAACATGGAGGAGACCATCTATCAGAACTACCAGCGTATCCGAATCCAGGAG
AGTCCAGGCAAAGTGGCGGCTGGCCGGCTGCCCCGCTCCAAGGACGCCATTCTCCTCGCA
GATCTGGTGGACAGCTGCAAGCCAGGAGACGAGATAGAGCTGACTGGCATCTATCACAAC
AACTATGATGGCTCCCTCAACACTGCCAATGGCTTCCCTGTCTTTGCCACTGTCATCCTA
GCCAACCACGTGGCCAAGAAGGACAACAAGGTTGCTGTAGGGGAACTGACCGATGAAGAT
GTGAAGATGATCACTAGCCTCTCCAAGGATCAGCAGATCGGAGAGAAGATCTTTGCCAGC
ATTGCTCCTTCCATCTATGGTCATGAAGACATCAAGAGAGGCCTGGCTCTGGCCCTGTTC
GGAGGGGAGCCCAAAAACCCAGGTGGCAAGCACAAGGTACGTGGTGATATCAACGTGCTC
TTGTGCGGAGACCCTGGCACAGCGAAGTCGCAGTTTCTCAAGTATATTGAGAAAGTGTCC
AGCCGAGCCATCTTCACCACTGGCCAGGGGGCGTCGGCTGTGGGCCTCACGGCGTATGTC
CAGCGGCACCCTGTCAGCAGGGAGTGGACCTTGGAGGCTGGGGCCCTGGTTCTGGCTGAC
CGAGGAGTGTGTCTCATTGATGAATTTGACAAGATGAATGACCAGGACAGAACCAGCATC
CATGAGGCCATGGAGCAACAGAGCATCTCCATCTCGAAGGCTGGCATCGTCACCTCCCTG
CAGGCTCGCTGCACGGTCATTGCTGCCGCCAACCCCATAGGAGGGCGCTACGACCCCTCG
CTGACTTTCTCTGAGAACGTGGACCTCACAGAGCCCATCATCTCACGCTTTGACATCCTG
TGTGTGGTGAGGGACACCGTGGACCCAGTCCAGGACGAGATGCTGGCCCGCTTCGTGGTG
GGCAGCCACGTCAGACACCACCCCAGCAACAAGGAGGAGGAGGGGCTGGCCAATGGCAGC
GCTGCTGAGCCCGCCATGCCCAACACGTATGGCGTGGAGCCCCTGCCCCAGGAGGTCCTG
AAGAAGTACATCATCTACGCCAAGGAGAGGGTCCACCCGAAGCTCAACCAGATGGACCAG
GACAAGGTGGCCAAGATGTACAGTGACCTGAGGAAAGAATCTATGGCGACAGGCAGCATC
CCCATTACGGTGCGGCACATCGAGTCCATGATCCGCATGGCGGAGGCCCACGCGCGCATC
CATCTGCGGGACTATGTGATCGAAGACGACGTCAACATGGCCATCCGCGTGATGCTGGAG
AGCTTCATAGACACACAGAAGTTCAGCGTCATGCGCAGCATGCGCAAGACTTTTGCCCGC
TACCTTTCATTCCGGCGTGACAACAATGAGCTGTTGCTCTTCATACTGAAGCAGTTAGTG
GCAGAGCAGGTGACATATCAGCGCAACCGCTTTGGGGCCCAGCAGGACACTATTGAGGTC
CCTGAGAAGGACTTGGTGGATAAGGCTCGTCAGATCAACATCCACAACCTCTCTGCATTT
TATGACAGTGAGCTCTTCAGGATGAACAAGTTCAGCCACGACCTGAAAAGGAAAATGATC
CTGCAGCAGTTC

Protein sequence predicted based on nucleotide sequence

MAESSESFTMASSPAQRRRGNDPLTSSPGRSSRRTDALTSSPGRDLPPFEDESEGLLGTE
GPLEEEEDGEELIGDGMERDYRAIPELDAYEAEGLALDDEDVEELTASQREAAERAMRQR
DREAGRGLGRMRRGLLYDSDEEDEERPARKRRQVERATEDGEEDEEMIESIENLEDLKGH
SVREWVSMAGPRLEIHHRFKNFLRTHVDSHGHNVFKERISDMCKENRESLVVNYEDLAAR
EHVLAYFLPEAPAELLQIFDEAALEVVLAMYPKYDRITNHIHVRISHLPLVEELRSLRQL
HLNQLIRTSGVVTSCTGVLPQLSMVKYNCNKCNFVLGPFCQSQNQEVKPGSCPECQSAGP
FEVNMEETIYQNYQRIRIQESPGKVAAGRLPRSKDAILLADLVDSCKPGDEIELTGIYHN
NYDGSLNTANGFPVFATVILANHVAKKDNKVAVGELTDEDVKMITSLSKDQQIGEKIFAS
IAPSIYGHEDIKRGLALALFGGEPKNPGGKHKVRGDINVLLCGDPGTAKSQFLKYIEKVS
SRAIFTTGQGASAVGLTAYVQRHPVSREWTLEAGALVLADRGVCLIDEFDKMNDQDRTSI
HEAMEQQSISISKAGIVTSLQARCTVIAAANPIGGRYDPSLTFSENVDLTEPIISRFDIL
CVVRDTVDPVQDEMLARFVVGSHVRHHPSNKEEEGLANGSAAEPAMPNTYGVEPLPQEVL
KKYIIYAKERVHPKLNQMDQDKVAKMYSDLRKESMATGSIPITVRHIESMIRMAEAHARI
HLRDYVIEDDVNMAIRVMLESFIDTQKFSVMRSMRKTFARYLSFRRDNNELLLFILKQLV
AEQVTYQRNRFGAQQDTIEVPEKDLVDKARQINIHNLSAFYDSELFRMNKFSHDLKRKMI
LQQF

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable