Gene:
Gene Symbol:
BRIP1
Gene Name:
BRCA1 interacting protein C-terminal helicase 1
Original Clone ID:
ccsbBroad304_09143
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00440250
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTCTTCAATGTGGTCTGAATATACAATTGGTGGGGTGAAGATTTACTTTCCTTATAAA
GCTTACCCGTCACAGCTTGCTATGATGAATTCTATTCTCAGAGGATTAAACAGCAAGCAA
CATTGTTTGTTGGAGAGTCCCACAGGAAGTGGAAAAAGCTTAGCCTTACTTTGTTCTGCT
TTAGCATGGCAACAATCTCTTAGTGGGAAACCAGCAGATGAGGGCGTAAGTGAAAAAGCT
GAAGTACAATTGTCATGTTGTTGTGCATGCCATTCAAAGGATTTTACAAACAATGACATG
AACCAAGGAACTTCACGTCATTTCAACTATCCAAGCACACCACCTTCTGAAAGAAATGGC
ACTTCATCAACTTGTCAAGACTCCCCTGAAAAAACCACTCTGGCTGCAAAGTTATCTGCT
AAGAAACAGGCATCCATATACAGAGATGAAAATGATGATTTTCAAGTAGAGAAGAAAAGA
ATTCGACCCTTAGAAACTACACAGCAGATTAGAAAACGTCATTGCTTTGGAACAGAAGTA
CACAATTTGGATGCAAAAGTTGATTCAGGAAAGACTGTAAAACTCAACTCTCCACTGGAA
AAGATAAACTCCTTTTCGCCACAGAAACCCCCTGGCCACTGTTCTAGGTGCTGTTGTTCT
ACTAAACAAGGAAACAGTCAAGAGTCATCGAATACCATTAAGAAGGATCATACAGGGAAA
TCCAAGATACCCAAAATATATTTTGGGACACGCACACACAAGCAGATTGCTCAGATTACT
AGAGAGCTCCGGAGGACGGCATATTCAGGGGTTCCAATGACTATTCTTTCCAGCAGGGAT
CATACTTGTGTCCATCCTGAGGTAGTCGGTAACTTCAACAGAAATGAGAAGTGCATGGAA
TTGCTAGATGGGAAAAACGGAAAATCCTGCTATTTTTATCATGGAGTTCATAAAATTAGT
GATCAGCACACATTACAGACTTTCCAAGGGATGTGCAAAGCCTGGGATATAGAAGAACTT
GTCAGCCTGGGGAAGAAACTAAAGGCCTGTCCATATTACACAGCCCGAGAACTAATACAA
GATGCTGACATCATATTTTGTCCCTACAACTATCTTCTAGATGCACAAATAAGGGAAAGT
ATGGATTTAAATCTGAAAGAACAGGTTGTCATTTTAGATGAAGCTCATAACATCGAGGAC
TGTGCTCGGGAATCAGCAAGTTACAGTGTAACAGAAGTTCAGCTTCGGTTTGCTCGGGAT
GAACTAGATAGTATGGTCAACAATAATATAAGGAAGAAAGATCATGAACCCCTACGAGCT
GTGTGCTGTAGCCTCATTAATTGGTTAGAAGCAAACGCTGAATATCTTGTAGAAAGAGAT
TATGAATCAGCTTGTAAAATATGGAGTGGAAATGAAATGCTCTTAACTTTACACAAAATG
GGTATCACCACTGCTACTTTTCCCATTTTGCAGGGACATTTTTCTGCTGTTCTTCAAAAA
GAGGAAAAAATCTCACCAATTTATGGTAAAGAGGAGGCAAGAGAAGTACCTGTTATTAGT
GCATCAACTCAAATAATGCTTAAAGGACTTTTTATGGTACTTGACTATCTTTTTAGGCAA
AATAGCAGATTTGCAGATGATTATAAAATTGCGATTCAACAGACTTACTCCTGGACAAAT
CAGATTGATATTTCAGACAAAAATGGGTTGTTGGTTCTACCAAAAAATAAGAAACGTTCA
CGACAGAAAACTGCAGTTCATGTGCTAAACTTTTGGTGCTTAAATCCAGCTGTGGCCTTT
TCAGATATTAATGGCAAAGTTCAGACCATTGTTTTGACATCTGGTACATTATCACCAATG
AAATCCTTTTCGTCAGAACTTGGTGTTACATTTACTATCCAGCTGGAGGCTAATCATATC
ATTAAAAATTCACAGGTTTGGGTTGGTACCATTGGGTCAGGCCCCAAGGGTCGGAATCTC
TGTGCTACCTTCCAGAATACTGAAACATTTGAGTTCCAAGATGAAGTGGGAGCACTTTTG
TTATCTGTGTGCCAGACTGTGAGCCAAGGAATTTTGTGTTTCTTGCCATCTTACAAGTTA
TTAGAAAAATTAAAAGAACGTTGGCTCTCTACTGGTTTATGGCATAATCTGGAGTTGGTG
AAGACAGTCATTGTAGAACCACAGGGAGGAGAAAAAACAAATTTTGATGAATTACTGCAG
GTGTACTATGACGCAATCAAATACAAAGGAGAGAAAGATGGAGCTCTCCTGGTAGCAGTT
TGTCGTGGTAAAGTGAGTGAGGGTCTGGATTTCTCAGATGACAATGCCCGTGCTGTCATA
ACAATAGGAATTCCTTTTCCAAATGTGAAAGATCTACAGGTTGAACTAAAACGACAATAC
AATGACCACCATTCAAAATTGAGAGGTCTTCTACCTGGCCGTCAGTGGTATGAAATTCAA
GCATACAGGGCCTTAAACCAGGCCCTTGGTAGATGTATTAGACACAGAAATGATTGGGGA
GCTCTTATTCTAGTGGATGATCGCTTTAGGAATAACCCAAGTCGCTATATATCTGGACTT
TCTAAATGGGTACGGCAGCAGATTCAGCACCATTCAACCTTTGAAAGTGCACTGGAGTCC
TTGGCTGAATTTTCCAAAAAGCATCAAAAAGTTCTTAATGTATCCATAAAGGACAGAACC
AATATACAGGACAATGAGTCTACACTTGAAGTGACCTCTTTAAAGTACAGTACCCCACCT
TATTTACTGGAAGCAGCAAGTCATCTATCACCAGAAAATTTTGTGGAAGATGAAGCAAAG
ATATGTGTCCAGGAACTACAGTGTCCTAAAATTATTACCAAAAATTCACCTCTACCAAGT
AGCATTATCTCCAGAAAGGAGAAAAATGATCCAGTATTCCTGGAAGAAGCAGGGAAAGCA
GAAAAAATTGTGATTTCCAGATCCACAAGCCCAACTTTCAACAAACAAACAAAGAGAGTT
AGCTGGTCAAGCTTTAATTCTTTGGGACAGTATTTTACTGGTAAAATACCGAAGGCAACA
CCTGAGCTCGGGTCATCAGAGAATAGTGCCTCTAGTCCTCCCCGTTTCAAAACAGAGAAG
ATGGAAAGTAAAACTGTTTTGCCCTTCACTGATAAATGTGAATCCTCAAATCTGACAGTA
AACACATCGTTTGGATCATGCCCTCAATCAGAAACCATTATTTCATCATTAAAGATTGAT
GCCACCCTTACTAGAAAAAATCATTCTGAACATCCGCTCTGTTCTGAAGAAGCCCTGGAT
CCAGACATTGAATTGTCTCTAGTAAGTGAAGAAGATAAACAGTCCACTTCAAATAGAGAT
TTTGAAACAGAAGCAGAAGATGAATCTATCTATTTTACACCTGAACTTTACGATCCTGAA
GATACAGATGAAGAAAAAAATGACCTAGCTGAAACTGATAGAGGAAATAGATTGGCTAAC
AATTCAGATTGCATTTTAGCTAAAGACCTTTTTGAAATTAGAACTATAAAAGAAGTAGAT
TCAGCCAGAGAAGTGAAAGCTGAGGATTGCATAGATACAAAGTTGAATGGAATTCTGCAT
ATTGAAGAAAGTAAAATTGATGACATTGATGGTAATGTAAAAACAACTTGGATAAATGAA
CTGGAACTGGGAAAAACTCATGAAATAGAAATAAAGAACTTTAAACCATCTCCTTCCAAA
AATAAAGGCATGTTTCCTGGTTTTAAG
Protein sequence predicted based on nucleotide sequence
MSSMWSEYTIGGVKIYFPYKAYPSQLAMMNSILRGLNSKQHCLLESPTGSGKSLALLCSA LAWQQSLSGKPADEGVSEKAEVQLSCCCACHSKDFTNNDMNQGTSRHFNYPSTPPSERNG TSSTCQDSPEKTTLAAKLSAKKQASIYRDENDDFQVEKKRIRPLETTQQIRKRHCFGTEV HNLDAKVDSGKTVKLNSPLEKINSFSPQKPPGHCSRCCCSTKQGNSQESSNTIKKDHTGK SKIPKIYFGTRTHKQIAQITRELRRTAYSGVPMTILSSRDHTCVHPEVVGNFNRNEKCME LLDGKNGKSCYFYHGVHKISDQHTLQTFQGMCKAWDIEELVSLGKKLKACPYYTARELIQ DADIIFCPYNYLLDAQIRESMDLNLKEQVVILDEAHNIEDCARESASYSVTEVQLRFARD ELDSMVNNNIRKKDHEPLRAVCCSLINWLEANAEYLVERDYESACKIWSGNEMLLTLHKM GITTATFPILQGHFSAVLQKEEKISPIYGKEEAREVPVISASTQIMLKGLFMVLDYLFRQ NSRFADDYKIAIQQTYSWTNQIDISDKNGLLVLPKNKKRSRQKTAVHVLNFWCLNPAVAF SDINGKVQTIVLTSGTLSPMKSFSSELGVTFTIQLEANHIIKNSQVWVGTIGSGPKGRNL CATFQNTETFEFQDEVGALLLSVCQTVSQGILCFLPSYKLLEKLKERWLSTGLWHNLELV KTVIVEPQGGEKTNFDELLQVYYDAIKYKGEKDGALLVAVCRGKVSEGLDFSDDNARAVI TIGIPFPNVKDLQVELKRQYNDHHSKLRGLLPGRQWYEIQAYRALNQALGRCIRHRNDWG ALILVDDRFRNNPSRYISGLSKWVRQQIQHHSTFESALESLAEFSKKHQKVLNVSIKDRT NIQDNESTLEVTSLKYSTPPYLLEAASHLSPENFVEDEAKICVQELQCPKIITKNSPLPS SIISRKEKNDPVFLEEAGKAEKIVISRSTSPTFNKQTKRVSWSSFNSLGQYFTGKIPKAT PELGSSENSASSPPRFKTEKMESKTVLPFTDKCESSNLTVNTSFGSCPQSETIISSLKID ATLTRKNHSEHPLCSEEALDPDIELSLVSEEDKQSTSNRDFETEAEDESIYFTPELYDPE DTDEEKNDLAETDRGNRLANNSDCILAKDLFEIRTIKEVDSAREVKAEDCIDTKLNGILH IEESKIDDIDGNVKTTWINELELGKTHEIEIKNFKPSPSKNKGMFPGFK
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: mammalian Marker: blasticidin
Host Type: mammalian Marker: blasticidin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLX304
Synonyms:
''
Sequencing Primer:
Forward:
CMV forward
Reverse:
None
Description:
with tag/fusion/marker assay, Gateway®, acceptor (destination), recombinational cloning clone, lentiviral infection expression with BlastR and T7 and attR1 and CMV and cPPT and V5 and AmpR and attR2 and CAT and ccdB and WPRE; ampicillin resistance in bacterial, blasticidin resistance in mammalian;
Comments:
None
Size (bp):
9377
Parent Vector:
None
Properties:
Gateway, acceptor (destination), lentiviral infection, recombinational cloning, with tag/fusion/marker
Author Name:
David Root
Publications:
PMID:
21706014
Title: A public genome-scale lentiviral expression library of human ORFs
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
polypurine tract
cPPT
human immunodeficiency virus type 1 central polypurine tract
3027
3042
promoter
CMV
CMV promoter
14
566
promoter
T7
T7 promoter
3690
3672
recombination site
attR1
595
719
recombination site
attR2
2282
2297
selectable marker
BlastR
Blasticidin resistance gene
8753
9148
selectable marker
AmpR
Ampicillin resistance gene
4444
5304
selectable marker
CAT
CAT/CamR Chloramphenical resistance
828
1487
selectable marker
ccdB
Control of cell death B gene
1829
2134
tag
V5
C-terminal V5 tag
2301
2342
trxn regulatory element
WPRE
woodchuck hepatitis post-transcriptional regulatory element
2386
2961
