MBD4 (Homo sapiens) in pLX304

Explanation of Terms

Gene:
Gene Symbol:  MBD4
Gene Name:  methyl-CpG binding domain protein 4
Original Clone ID:
N/A
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
Clone failed to grow from plate and tube sources
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00441703
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UNAVAILABLE
No restriction
Special MTA: None
Insert sequence: 1725nts
Open reading frame: 1 to 1725

ATGGGCACGACTGGGCTGGAGAGTCTGAGTCTGGGGGACCGCGGAGCTGCCCCCACCGTC
ACCTCTAGTGAGCGCCTAGTCCCAGACCCGCCGAATGACCTCCGCAAAGAAGATGTTGCT
ATGGAATTGGAAAGAGTGGGAGAAGATGAGGAACAAATGATGATAAAAAGAAGCAGTGAA
TGTAATCCCTTGCTACAAGAACCCATCGCTTCTGCTCAGTTTGGTGCTACTGCAGGAACA
GAATGCCGTAAGTCTGTCCCATGTGGATGGGAAAGAGTTGTGAAGCAAAGGTTATTTGGG
AAGACAGCAGGAAGATTTGATGTGTACTTTATCAGCCCACAAGGACTGAAGTTCAGATCC
AAAAGTTCACTTGCTAATTATCTTCACAAAAATGGAGAGACTTCTCTTAAGCCAGAAGAT
TTTGATTTTACTGTACTTTCTAAAAGGGGTATCAAGTCAAGATATAAAGACTGCAGCATG
GCAGCCCTGACATCCCATCTACAAAACCAAAGTAACAATTCAAACTGGAACCTCAGGACC
CGAAGCAAGTGCAAAAAGGATGTGTTTATGCCGCCAAGTAGTAGTTCAGAGTTGCAGGAG
AGCAGAGGACTCTCTAACTTTACTTCCACTCATTTGCTTTTGAAAGAAGATGAGGGTGTT
GATGATGTTAACTTCAGAAAGGTTAGAAAGCCCAAAGGAAAGGTGACTATTTTGAAAGGA
ATCCCAATTAAGAAAACTAAAAAAGGATGTAGGAAGAGCTGTTCAGGTTTTGTTCAAAGT
GATAGCAAAAGAGAATCTGTGTGTAATAAAGCAGATGCTGAAAGTGAACCTGTTGCACAA
AAAAGTCAGCTTGATAGAACTGTCTGCATTTCTGATGCTGGAGCATGTGGTGAGACCCTC
AGTGTGACCAGTGAAGAAAACAGCCTTGTAAAAAAAAAAAAGAAAGATCATTGAGTTCAG
GATCAAATTTTTGTTCTGAACAAAAAACTTCTGGCATCATAAACAAATTTTGTTCAGCCA
AAGACTCAGAACACAACGAGAAGTATGAGGATACCTTTTTAGAATCTGAAGAAATCGGAA
CAAAAGTAGAAGTTGTGGAAAGGAAAGAACATTTGCATACTGACATTTTAAAACGTGGCT
CTGAAATGGACAACAACTGCTCACCAACCAGGAAAGACTTCACTGAAGATACCATCCCAC
GAACACAGATAGAAAGAAGGAAAACAAGCCTGTATTTTTCCAGCAAATATAACAAAGAAG
CTCTTAGCCCCCCACGACGTAAAGCCTTTAAGAAATGGACACCTCCTCGGTCACCTTTTA
ATCTCGTTCAAGAAACACTTTTTCATGATCCATGGAAGCTTCTCATCGCTACTATATTTC
TCAATCGGACCTCAGGCAAAATGGCAATACCTGTGCTTTGGAAGTTTCTGGAGAAGTATC
CTTCAGCTGAGGTAGCAAGAACCGCAGACTGGAGAGATGTGTCAGAACTTCTTAAACCTC
TTGGTCTCTACGATCTTCGGGCAAAAACCATTGTCAAGTTCTCAGATGAATACCTGACAA
AGCAGTGGAAGTATCCAATTGAGCTTCATGGGATTGGTAAATATGGCAACGACTCTTACC
GAATTTTTTGTGTCAATGAGTGGAAGCAGGTGCACCCTGAAGACCACAAATTAAATAAAT
ATCATGACTGGCTTTGGGAAAATCATGAAAAATTAAGTCTATCT

Protein sequence predicted based on nucleotide sequence

MGTTGLESLSLGDRGAAPTVTSSERLVPDPPNDLRKEDVAMELERVGEDEEQMMIKRSSE
CNPLLQEPIASAQFGATAGTECRKSVPCGWERVVKQRLFGKTAGRFDVYFISPQGLKFRS
KSSLANYLHKNGETSLKPEDFDFTVLSKRGIKSRYKDCSMAALTSHLQNQSNNSNWNLRT
RSKCKKDVFMPPSSSSELQESRGLSNFTSTHLLLKEDEGVDDVNFRKVRKPKGKVTILKG
IPIKKTKKGCRKSCSGFVQSDSKRESVCNKADAESEPVAQKSQLDRTVCISDAGACGETL
SVTSEENSLVKKKKKDH*VQDQIFVLNKKLLAS*TNFVQPKTQNTTRSMRIPF*NLKKSE
QK*KLWKGKNICILTF*NVALKWTTTAHQPGKTSLKIPSHEHR*KEGKQACIFPANITKK
LLAPHDVKPLRNGHLLGHLLISFKKHFFMIHGSFSSLLYFSIGPQAKWQYLCFGSFWRSI
LQLR*QEPQTGEMCQNFLNLLVSTIFGQKPLSSSQMNT*QSSGSIQLSFMGLVNMATTLT
EFFVSMSGSRCTLKTTN*INIMTGFGKIMKN*VY

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
>4 errors; >4 non-synonomous errors;
 early stop codon; insertion or deletion in coding sequence
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable