UBE4A (Homo sapiens) in pLX304

Explanation of Terms

Gene:
Gene Symbol:  UBE4A
Gene Name:  ubiquitination factor E4A
Original Clone ID:
ccsbBroad304_11359
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00443166
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3199nts
Open reading frame: 1 to 3199

ATGACAGACCAGGAGAATAACAACAACATCTCAAGTAACCCCTTTGCTGCTCTTTTTGGC
TCCCTGGCTGATGCCAAACAGTTTGCGGCAATCCAAAAAGAGCAGCTGAAGCAACAATCT
GATGAACTCCCAGCTAGCCCAGATGACTCGGATAATAGCGTGTCAGAGAGCCTGGATGAA
TTCGATTACTCTGTGGCTGAGATTAGCCGCTCATTCCGATCACAGCAGGAAATATGTGAG
CAACTCAACATCAATCACATGATCCAAAGGATCTTCCTTATTACTCTGGACAACAGTGAT
CCCAGCTTGAAAAGCGGGAATGGCATCCCTAGCCGTTGTGTGTATTTGGAAGAAATGGCA
GTAGAGCTAGAAGATCAAGACTGGCTTGATATGAGCAATGTTGAGCAGGCCCTCTTCGCT
CGCTTATTACTTCAAGATCCAGGCAACCACTTAATTAACATGACTTCTTCTACAACGCTA
AATCTCTCTGCTGATCGAGATGCAGGAGAGAGGCACATTTTTTGTTACCTTTACTCCTGC
TTCCAGAGAGCCAAGGAAGAGATTACCAAAGTTCCAGAGAACCTGCTACCCTTTGCAGTG
CAGTGCAGAAACCTCACTGTGTCCAATACCCGAACAGTTCTTCTCACCCCAGAGATCTAT
GTTGACCAAAACATCCATGAGCAACTGGTAGATTTGATGTTAGAAGCCATCCAGGGAGCC
CATTTTGAAGATGTAACTGAGTTTCTGGAAGAGGTCATTGAAGCCTTGATATTGGATGAG
GAAGTTAGAACATTTCCAGAAGTCATGATTCCAGTGTTTGATATTTTATTGGGCCGAATA
AAAGATCTAGAGCTCTGTCAGATCCTTTTGTATGCATATCTGGATATTCTTCTCTATTTC
ACTAGGCAAAAAGATATGGCAAAGGTTTTTGTAGAATACATTCAGCCCAAGGACCCTACC
AATGGGCAAATGTACCAGAAGACCTTGCTGGGAGTAATTCTGAGTATCTCCTGCTTATTA
AAGACTCCGGGTGTTGTAGAAAATCATGGCTACTTTTTGAATCCATCTCGTTCCAGCCCC
CAGGAGATCAAAGTACAGGAGGCCAACATCCATCAGTTCATGGCTCAGTTCCACGAAAAG
ATCTACCAGATGCTGAAGAACTTACTCCAGCTCTCTCCAGAAACCAAACACTGTATCTTG
TCCTGGCTTGGAAACTGTTTGCATGCAAATGCAGGCCGCACCAAGATTTGGGCCAATCAG
ATGCCAGAAATCTTTTTCCAAATGTATGCCTCAGATGCTTTCTTTCTGAATCTGGGTGCT
GCTCTCCTGAAGCTATGCCAGCCATTTTGCAAACCCAGATCCTCTCGGCTCCTCACCTTT
AATCCCACATACTGTGCCCTCAAGGAGTTGAATGATGAAGAACGAAAAATTAAAAATGTA
CACATGAGAGGTTTGGACAAAGAAACCTGTTTGATCCCAGCTGTGCAGGAGCCGAAGTTT
CCACAGAACTACAACCTTGTAACAGAGAACCTTGCTCTGACAGAGTACACCTTGTACTTG
GGATTTCACAGGTTGCATGATCAGATGGTAAAAATCAACCAAAATCTGCATCGGCTGCAG
GTTGCCTGGCGGGATGCTCAGCAAAGTTCTAGCCCTGCTGCTGACAATCTTCGTGAGCAG
TTTGAACGACTGATGACCATCTATCTTTCTACCAAGACTGCCATGACAGAGCCACAAATG
CTACAAAACTGCCTAAACTTGCAGGTGTCCATGGCTGTTCTACTGGTTCAACTGGCCATA
GGCAATGAGGGCTCACAGCCAATAGAGCTAACCTTTCCTTTGCCAGATGGCTACAGCTCT
TTGGCTTATGTGCCAGAATTTTTTGCAGATAACCTGGGTGATTTTCTCATTTTTCTCCGC
CGCTTTGCCGATGACATTTTGGAGACATCAGCAGATTCCCTGGAGCATGTCCTTCACTTT
ATCACCATTTTCACTGGAAGCATAGAAAGAATGAAGAATCCCCACCTGAGGGCCAAACTA
GCAGAGGTGTTGGAAGCAGTGATGCCCCACCTGGATCAGACCCCAAATCCCTTGGTATCC
AGTGTGTTCCACCGGAAACGTGTGTTCTGCAACTTTCAGTATGCACCCCAACTTGCAGAG
GCTCTAATCAAGGTTTTTGTGGACATCGAATTTACAGGAGACCCCCATCAATTTGAACAG
AAGTTTAATTACCGCCGTCCCATGTATCCTATCCTAAGATACATGTGGGGGACAGATACC
TATCGGGAGAGCATTAAGGATTTGGCTGACTATGCCTCTAAGAATTTAGAAGCCATGAAT
CCCCCACTTTTCCTCCGCTTTCTTAACCTGCTAATGAATGATGCCATCTTCCTTTTGGAT
GAAGCCATACAGTATTTGAGCAAGATAAAGATTCAGCAAATTGAGAAGGATCGAGGTGAA
TGGGATAGTCTGACTCCAGAAGCCCGCCGAGAAAAGGAGGCTGGCCTACAGATGTTTGGA
CAGCTGGCACGTTTCCATAACATCATGTCCAATGAAACAATCGGTACCCTTGCCTTTCTC
ACATCAGAGATCAAGTCACTCTTTGTGCATCCCTTCCTGGCTGAGCGCATCATCTCCATG
TTGAACTACTTCCTGCAACACCTGGTTGGCCCCAAGATGGGTGCCTTAAAAGTCAAGGAC
TTCAGCGAATTTGACTTCAAACCCCAGCAGCTTGTATCAGATATCTGCACTATCTACTTA
AATCTTGGGGATGAGGAGAATTTCTGTGCCACTGTGCCCAAGGATGGACGTTCCTATTCC
CCAACTCTCTTTGCACAGACAGTTCGAGTCTTGAAGAAAATAAATAAGCCTGGGAATATG
ATTATGGCTTTCAGCAACTTGGCAGAGAGAATCAAGTCTCTTGCAGACCTCCAACAACAG
GAAGAGGAAACCTATGCAGATGCCTGTGATGAGTTCCTGGATCCCATTATGAGCACACTG
ATGTGTGACCCTGTGGTGCTGCCATCTTCCAGAGTCACTGTGGATAGATCCACCATTGCA
AGACATTTGCTCAGTGACCAAACAGATCCCTTTAACCGTAGTCCCCTCACCATGGACCAG
ATCCGGCCAAACACAGAACTAAAAGAAAAAATCCAACGGTGGCTTGCAGAGAGGAAACAA
CAAAAGGAGCAACTTGAA

Protein sequence predicted based on nucleotide sequence

MTDQENNNNISSNPFAALFGSLADAKQFAAIQKEQLKQQSDELPASPDDSDNSVSESLDE
FDYSVAEISRSFRSQQEICEQLNINHMIQRIFLITLDNSDPSLKSGNGIPSRCVYLEEMA
VELEDQDWLDMSNVEQALFARLLLQDPGNHLINMTSSTTLNLSADRDAGERHIFCYLYSC
FQRAKEEITKVPENLLPFAVQCRNLTVSNTRTVLLTPEIYVDQNIHEQLVDLMLEAIQGA
HFEDVTEFLEEVIEALILDEEVRTFPEVMIPVFDILLGRIKDLELCQILLYAYLDILLYF
TRQKDMAKVFVEYIQPKDPTNGQMYQKTLLGVILSISCLLKTPGVVENHGYFLNPSRSSP
QEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPETKHCILSWLGNCLHANAGRTKIWANQ
MPEIFFQMYASDAFFLNLGAALLKLCQPFCKPRSSRLLTFNPTYCALKELNDEERKIKNV
HMRGLDKETCLIPAVQEPKFPQNYNLVTENLALTEYTLYLGFHRLHDQMVKINQNLHRLQ
VAWRDAQQSSSPAADNLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAI
GNEGSQPIELTFPLPDGYSSLAYVPEFFADNLGDFLIFLRRFADDILETSADSLEHVLHF
ITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQTPNPLVSSVFHRKRVFCNFQYAPQLAE
ALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDTYRESIKDLADYASKNLEAMN
PPLFLRFLNLLMNDAIFLLDEAIQYLSKIKIQQIEKDRGEWDSLTPEARREKEAGLQMFG
QLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKD
FSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNM
IMAFSNLAERIKSLADLQQQEEETYADACDEFLDPIMSTLMCDPVVLPSSRVTVDRSTIA
RHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKQQKEQLE

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable