NT5C1B (Homo sapiens) in pLX304

Explanation of Terms

Gene:
Gene Symbol:  NT5C1B-RDH14
Gene Name:  NT5C1B-RDH14 readthrough
Original Clone ID:
ccsbBroad304_14343
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00443646
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1838nts
Open reading frame: 1 to 1838

ATGAGTCAAACATCTCTCAAACAGAAAAAGAATGAGCCTGGAATGAGGTCCTCAAAAGAG
AGTCTAGAAGCAGAAAAAAGAAAGGAATCTGACAAAACAGTAGTTCGTCTGAGCAATCAG
ATGAGGCGTGCAGTCAATCCGAATCACTCGCTGAGATGTTGCCCCTTCCAGGGTCACTCG
TCGTGTAGACGCTGCCTTTGTGCAGCTGAGGGAACAGCCCTTGGCCCCTGCCACACAATA
CGTATTTATATTCACATGTGCCTGTTGTGGGAGCAGGGCCAGCAGATCACCATGATGAGG
GGATCACAAGAATCATCACTGCGGAAGACAGACTCTCGAGGGTACCTTGTGCGCAGTCAA
TGGTCTAGAATATCCCGGAGCCCATCCACCAAGGCTCCATCCATAGATGAGCCTAGAAGC
AGGAACACCAGTGCTAAGGTAGAGCTCCCCAGCAGCTCCACGAGCTCCCGGGACTCCATC
CACCTCCCCAAGCCTGCATGACTCCTCACCGCCGCCGCTGTCCGGGCAGCCCTCGCTCCA
GCCACCCGCGTCGCCCCAGCTGCCCCGGTCGCTGGACTCGCGGCCTCCCACGCCCCCAGA
GCCCGATCCTGGCTCCCGGCGCAGCACCAAAATGCAAGAGAATCCGGAGGCCTGGGCCCA
AGGCATCGTGCGGGAAATCCGCCAGACCCGGGACTCGCAGCCGCTGGAATATTCGCGCAC
GTCCCCCACCGAGTGGAAGTCCTCCAGCCAGCGCAGGGGGATCTACCCCGCCTCCACCCA
GCTGGACCGCAACTCTCTGTCCGAGCAGCAGCAGCAGCAGCGGGAGGACGAGGACGACTA
CGAGGCTGCCTACTGGGCATCCATGAGGTCGTTCTACGAGAAGAACCCGAGCTGCTCGCG
CCCCTGGCCGCCCAAACCCAAGAACGCCATCACCATTGCTCTCTCATCCTGCGCGCTCTT
CAACATGGTGGACGGCAGGAAAATCTACGAGCAAGAGGGTCTGGAAAAGTACATGGAGTA
TCAGCTCACCAATGAGAACGTCATCCTGACCCCGGGCCCGGCGTTCCGCTTCGTCAAGGC
ACTACAGTATGTCAATGCTAGACTCCGTGATCTATATCCTGATGAACAGGACTTATTTGA
TATTGTACTGATGACTAATAACCATGCCCAAGTGGGAGTGCGGCTTATAAACAGCGTCAA
TCACTACGGCTTACTGATTGACCGCTTCTGTCTGACCGGGGGAAAAGACCCCATTGGCTA
TTTGAAGGCATATCTTACCAACTTGTATATTGCTGCAGATTCTGAAAAAGTGCAAGAGGC
AATACAAGAAGGTATTGCCTCTGCGACAATGTTTGATGGAGCCAAAGACATGGCTTACTG
TGACACTCAGCTCCGTGTAGCCTTTGATGGGGATGCTGTCCTCTTCTCTGATGAGTCTGA
ACATTTTACCAAGGAGCATGGGCTCGACAAATTCTTCCAGTATGATACATTATGTGAAAG
TAAGCCTCTTGCTCAGGGTCCCCTAAAAGGCTTTCTGGAAGATTTAGGCAGACTGCAAAA
GAAGTTCTATGCCAAAAATGAACGGTTACTTTGTCCTATCAGGACCTACCTGGTTACAGC
TAGGAGTGCAGCCAGTTCAGGCGCCCGTGTGCTGAAGACCCTTCGACGCTGGGGTCTAGA
GATAGACGAAGCTCTTTTCCTTGCTGGAGCCCCCAAAAGTCCCATCTTGGTGAAGATCCG
GCCCCACATCTTCTTTGATGACCACATGTTCCACATTGAAGGGGCACAGAGGTTAGGTTC
CATCGCAGCTTATGGCTTTAATAAAAAATTCAGTAGT

Protein sequence predicted based on nucleotide sequence

MSQTSLKQKKNEPGMRSSKESLEAEKRKESDKTVVRLSNQMRRAVNPNHSLRCCPFQGHS
SCRRCLCAAEGTALGPCHTIRIYIHMCLLWEQGQQITMMRGSQESSLRKTDSRGYLVRSQ
WSRISRSPSTKAPSIDEPRSRNTSAKVELPSSSTSSRDSIHLPKPA*LLTAAAVRAALAP
ATRVAPAAPVAGLAASHAPRARSWLPAQHQNARESGGLGPRHRAGNPPDPGLAAAGIFAH
VPHRVEVLQPAQGDLPRLHPAGPQLSVRAAAAAAGGRGRLRGCLLGIHEVVLREEPELLA
PLAAQTQERHHHCSLILRALQHGGRQENLRARGSGKVHGVSAHQ*ERHPDPGPGVPLRQG
TTVCQC*TP*SIS**TGLI*YCTDD**PCPSGSAAYKQRQSLRLTD*PLLSDRGKRPHWL
FEGISYQLVYCCRF*KSARGNTRRYCLCDNV*WSQRHGLL*HSAPCSL*WGCCPLL**V*
TFYQGAWARQILPV*YIM*K*ASCSGSPKRLSGRFRQTAKEVLCQK*TVTLSYQDLPGYS
*ECSQFRRPCAEDPSTLGSRDRRSSFPCWSPQKSHLGEDPAPHLL**PHVPH*RGTEVRF
HRSLWL**KIQ*

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
>4 errors; >4 non-synonomous errors;
 early stop codon; insertion or deletion in coding sequence
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable